CCR7 antibody

Catalog No. ABIN2689059

Product Details anti-CCR7 Antibody

Target: CCR7 (Chemokine (C-C Motif) Receptor 7 (CCR7))

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This CCR7 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Brand: BD Pharmingen™

Characteristics: The monoclonal antibody 3D12 reacts with the human CC chemokine receptor, CCR7. CCR7 (previously known as BLR-2, EBI-1 and CMKBR7), a seven-transmembrane, G-protein-coupled receptor, is the specific receptor for CC chemokines, MIP-3β/Exodus 3/ELC/ CCL19 and 6Ckine/Exodus 2/SLC/TCA4/CCL21. It has been shown that CCR7 mRNA is expressed mainly in lymphoid tissues including spleen, lymph nodes and tonsil. CCR7 mRNA was also detected in peripheral T and B lymphocytes, in bone marrow and cord blood CD34-positive cells and mature dendritic cells. The human CCR7 gene, unlike other CC chemokine receptor genes, has been mapped to chromosome 17q12. The immunogen used to generate 3D12 hybridoma was the N-terminus as well as parts of the second extracellular loop of human CCR7 protein. The monoclonal antibody 3D12 recognizes an epitode mapping to the N-terminus of human CCR7. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Detection of CCR7 expression on CD4 and CD8-positive human peripheral lymphocytes by purified anti-human CCR7 antibody 3D12. Human PBMC were stained with 0.25 μg/test of purified 3D12 using 3-step staining protocol outlined below and anti-human CD45RA-FITC (Cat. No. 555488). The data shown are derived from the CD4-positive (based on staining with anti-human CD4-APC, Cat. No. 555349, left panel) and CD8-positive (based on staining with anti-human CD8-APC, Cat. No. 555369, right panel) lymphocyte gated populations and displayed as bivariate dot plots.

BD Pharmingen™ Purified Rat Anti-Human CD197 (CCR7) - Purified - Clone 3D12 - Isotype Rat IgG2a, κ - Reactivity Hu - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human CCR7 protein

Clone: 3D12

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-CCR7 antibody (ABIN2689059)

Lipp, Burgstahler, Müller, Pevzner, Kremmer, Wolf, Förster: "Functional organization of secondary lymphoid organs by the chemokine system." in: Current topics in microbiology and immunology, Vol. 251, pp. 173-9, (2000) (PubMed).

Sallusto, Lenig, Förster, Lipp, Lanzavecchia: "Two subsets of memory T lymphocytes with distinct homing potentials and effector functions." in: Nature, Vol. 401, Issue 6754, pp. 708-12, (1999) (PubMed).

Yanagihara, Komura, Nagafune, Watarai, Yamaguchi: "EBI1/CCR7 is a new member of dendritic cell chemokine receptor that is up-regulated upon maturation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 6, pp. 3096-102, (1998) (PubMed).

Yoshida, Nagira, Imai, Baba, Takagi, Tabira, Akagi, Nomiyama, Yoshie: "EBI1-ligand chemokine (ELC) attracts a broad spectrum of lymphocytes: activated T cells strongly up-regulate CCR7 and efficiently migrate toward ELC." in: International immunology, Vol. 10, Issue 7, pp. 901-10, (1998) (PubMed).

Yoshida, Nagira, Kitaura, Imagawa, Imai, Yoshie: "Secondary lymphoid-tissue chemokine is a functional ligand for the CC chemokine receptor CCR7." in: The Journal of biological chemistry, Vol. 273, Issue 12, pp. 7118-22, (1998) (PubMed).

Yoshida, Imai, Hieshima, Kusuda, Baba, Kitaura, Nishimura, Kakizaki, Nomiyama, Yoshie: "Molecular cloning of a novel human CC chemokine EBI1-ligand chemokine that is a specific functional ligand for EBI1, CCR7." in: The Journal of biological chemistry, Vol. 272, Issue 21, pp. 13803-9, (1997) (PubMed).

Burgstahler, Kempkes, Steube, Lipp: "Expression of the chemokine receptor BLR2/EBI1 is specifically transactivated by Epstein-Barr virus nuclear antigen 2." in: Biochemical and biophysical research communications, Vol. 215, Issue 2, pp. 737-43, (1995) (PubMed).

Schweickart, Raport, Godiska, Byers, Eddy, Shows, Gray: "Cloning of human and mouse EBI1, a lymphoid-specific G-protein-coupled receptor encoded on human chromosome 17q12-q21.2." in: Genomics, Vol. 23, Issue 3, pp. 643-50, (1995) (PubMed).

Birkenbach, Josefsen, Yalamanchili, Lenoir, Kieff: "Epstein-Barr virus-induced genes: first lymphocyte-specific G protein-coupled peptide receptors." in: Journal of virology, Vol. 67, Issue 4, pp. 2209-20, (1993) (PubMed).

Target Details for CCR7

Target: CCR7 (Chemokine (C-C Motif) Receptor 7 (CCR7))

Alternative Name: CD197 CCR7 (CCR7 Products)

Synonyms: zgc:165629 antibody, ccr7-a antibody, CD197 antibody, Cdw197 antibody, Cmkbr7 antibody, EBI1 antibody, Ebi1h antibody, BLR2 antibody, CDw197 antibody, CMKBR7 antibody, chemokine (C-C motif) receptor 7 antibody, C-C motif chemokine receptor 7 antibody, C-C motif chemokine receptor 7 L homeolog antibody, ccr7 antibody, CCR7 antibody, ccr7.L antibody, Ccr7 antibody

Background: Synonyms: BLR-2, EBI-1, CMKBR7

Pathways: Regulation of Actin Filament Polymerization, Positive Regulation of Immune Effector Process