CD2 antibody (Biotin)

Catalog No. ABIN2689074

Product Details anti-CD2 Antibody (Biotin)

Target: CD2

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD2 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Brand: BD Pharmingen™

Characteristics: The RM2-5 antibody reacts with the immunoglobulin superfamily adhesion molecule CD2 (LFA-2), which is the major receptor for CD48 in the mouse and may be involved in T-cell activation, immunoregulation, and thymocyte maturation. In the mouse, CD2 is expressed on peripheral T lymphocytes, B lymphocytes, and NK cells, except a subpopulation of intraepithelial T lymphocytes. CD2 is present throughout mouse thymic ontogeny, except for distinct subsets of the CD4-CD8- early thymocytes. In the mouse bone marrow, CD2 is expressed on B220+ sIg+ CD43- pre-B cells, but not on CD43+ pro-B cells. RM2-5 antibody is one of a set of five anti-mouse CD2 mAbs which were classified into two groups according to their mutual competition in binding to cell surface CD2, and which block CD2-mediated cell-cell adhesion. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature.

BD Pharmingen™ Biotin Rat Anti-Mouse CD2 - Biotin - Clone RM2-5 - Isotype Rat IgG2b, λ - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse BALB/c Thymocytes

Clone: RM2

Isotype: IgG2b lambda

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-CD2 antibody (Biotin) (ABIN2689074)

Hayday, Theodoridis, Ramsburg, Shires: "Intraepithelial lymphocytes: exploring the Third Way in immunology." in: Nature immunology, Vol. 2, Issue 11, pp. 997-1003, (2001) (PubMed).

Kuo, El Guindy, Panwala, Hagan, Camerini: "Differential appearance of T cell subsets in the large and small intestine of neonatal mice." in: Pediatric research, Vol. 49, Issue 4, pp. 543-51, (2001) (PubMed).

Ayroldi, Migliorati, Cannarile, Moraca, Delfino, Riccardi: "CD2 rescues T cells from T-cell receptor/CD3 apoptosis: a role for the Fas/Fas-L system." in: Blood, Vol. 89, Issue 10, pp. 3717-26, (1997) (PubMed).

Cibotti, Punt, Dash, Sharrow, Singer: "Surface molecules that drive T cell development in vitro in the absence of thymic epithelium and in the absence of lineage-specific signals." in: Immunity, Vol. 6, Issue 3, pp. 245-55, (1997) (PubMed).

Davis, van der Merwe: "The structure and ligand interactions of CD2: implications for T-cell function." in: Immunology today, Vol. 17, Issue 4, pp. 177-87, (1997) (PubMed).

Masten, Yates, Pollard Koga, Lipscomb: "Characterization of accessory molecules in murine lung dendritic cell function: roles for CD80, CD86, CD54, and CD40L." in: American journal of respiratory cell and molecular biology, Vol. 16, Issue 3, pp. 335-42, (1997) (PubMed).

Rakasz, Hagen, Sandor, Lynch: "Gamma delta T cells of the murine vagina: T cell response in vivo in the absence of the expression of CD2 and CD28 molecules." in: International immunology, Vol. 9, Issue 1, pp. 161-7, (1997) (PubMed).

Teh, Killeen, Tarakhovsky, Littman, Teh: "CD2 regulates the positive selection and function of antigen-specific CD4- CD8+ T cells." in: Blood, Vol. 89, Issue 4, pp. 1308-18, (1997) (PubMed).

Criado, Feito, Rojo: "CD4-dependent and -independent association of protein tyrosine kinases to the T cell receptor/CD3 complex of CD4+ mouse T lymphocytes." in: European journal of immunology, Vol. 26, Issue 6, pp. 1228-34, (1996) (PubMed).

Papavasiliou, Misulovin, Suh, Nussenzweig: "The role of Ig beta in precursor B cell transition and allelic exclusion." in: Science (New York, N.Y.), Vol. 268, Issue 5209, pp. 408-11, (1995) (PubMed).

Rodewald, Awad, Moingeon, DAdamio, Rabinowitz, Shinkai, Alt, Reinherz: "Fc gamma RII/III and CD2 expression mark distinct subpopulations of immature CD4-CD8- murine thymocytes: in vivo developmental kinetics and T cell receptor beta chain rearrangement status." in: The Journal of experimental medicine, Vol. 177, Issue 4, pp. 1079-92, (1993) (PubMed).

Kato, Koyanagi, Okada, Takanashi, Wong, Williams, Okumura, Yagita: "CD48 is a counter-receptor for mouse CD2 and is involved in T cell activation." in: The Journal of experimental medicine, Vol. 176, Issue 5, pp. 1241-9, (1992) (PubMed).

Nakamura, Takahashi, Fukazawa, Koyanagi, Yokoyama, Kato, Yagita, Okumura: "Relative contribution of CD2 and LFA-1 to murine T and natural killer cell functions." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 145, Issue 11, pp. 3628-34, (1991) (PubMed).

Yagita, Nakamura, Asakawa, Matsuda, Tansyo, Iigo, Okumura: "CD2 expression in murine B cell lineage." in: International immunology, Vol. 1, Issue 1, pp. 94-8, (1991) (PubMed).

Yagita, Asakawa, Tansyo, Nakamura, Habu, Okumura: "Expression and function of CD2 during murine thymocyte ontogeny." in: European journal of immunology, Vol. 19, Issue 12, pp. 2211-7, (1990) (PubMed).

Yagita, Nakamura, Karasuyama, Okumura: "Monoclonal antibodies specific for murine CD2 reveal its presence on B as well as T cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 86, Issue 2, pp. 645-9, (1989) (PubMed).

Target Details for CD2

Target: CD2

Alternative Name: CD2 (CD2 Products)

Synonyms: CD2 antibody, LFA-2 antibody, Ly-37 antibody, Ly37 antibody, SRBC antibody, T11 antibody, CD2R antibody, LFA2 antibody, OX-34 antibody, OX34 antibody, CD2 molecule antibody, CD2 antigen antibody, Cd2 molecule antibody, CD2 antibody, Cd2 antibody

Background: Synonyms: LFA-2