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Kinesin Heavy Chain Member 2A (KIF2A) (N-Term) antibody

Details for Product No. ABIN268909, Supplier: Log in to see
  • C530030B14Rik
  • fj55b02
  • HK2
  • Kif2
  • KIF2
  • kif2-a
  • kinesin-II
  • Kns2
  • kns2
  • M-kinesin
  • wu:fj55b02
anti-Mouse (Murine) Kinesin Heavy Chain Member 2A antibody for Western Blotting
Human, Mammalian, Mouse (Murine), Rat (Rattus)
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
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Immunogen A recombinant segment of the N-terminal domain of human Kif2a.
Purification Whole antisera
Alternative Name KIF2A (KIF2A Antibody Abstract)
Background Kif2a is a kinesin-like motor protein that depolymerizes microtubules. It is found inseveral vertebrate cell types but is strongly expressed in neuronal cells. In dividing cellsit is required for bipolar spindle formation and chromosome segregation. In neuronalcells it is involved in neurite outgrowth. Alternate Names: anti-KIF2 antibody, anti-KIF-2 antibody, anti-Kinesin Heavy Chain Member.
Gene Symbol: KIF2A
Gene ID 3796, 16563, 84391
UniProt O00139
Research Area Cytoskeleton
Pathways Microtubule Dynamics
Application Notes This Kif2a antibody is useful for Western blot, Immunoprecipitation, and Immunocytochemistry/Immunofluorescence. A band at ~110 kDa can be detected by Western blot.
Recommended dilutions: Immunocytochemistry/Immunofluorescence 1:10000, Immunoprecipitation 1:10-1:500, Western Blot 1:10000
Protocol Western blot Protocol for Kif2a antibody
Western Blot Protocol:
1. Perform SDS-PAGE on samples to be analyzed, loading 40 µg of total protein per lane.
. Transfer proteins to membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
. Stain according to standard Ponceau S procedure (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
. Rinse the blot.
. Block the membrane using standard blocking buffer for at least 1 hour.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Dilute rabbit anti-Kif2a primary antibody in blocking buffer and incubate 1 hour at room temperature.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Apply the diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer three times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions.Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %.ICC/IF Protocol for Kif2a antibody Immunocytochemistry ProtocolCulture cells to appropriate density on suitable glass coverslips in 35 mm culture dishes or 6-well plates.
. Remove culture medium and add 10 % formalin to the dish. Fix at room temperature for 5-10 minutes.
. Remove the formalin and add 0.5 % Triton-X 100 in TBS to permeabilize the cells. Incubate for 5-10 minutes.
. Remove the permeabilization buffer and add wash buffer (i.e. PBS or PBS with 0.1 % Tween-20). Be sure to not let the specimen dry out. Gently wash three times for 10 minutes.
. Alternatively, cells can be fixed with -20C methanol for 10 min at room temperature. Remove the methanol and rehydrate in PBS for 10 min before proceeding.
. To block nonspecific antibody binding incubate in 10 % normal goat serum for 1 hour at room temperature.
. Add primary antibody at appropriate dilution and incubate at room temperature for 1 hour or at 4 degrees C overnight.
. Remove primary antibody and replace with wash buffer. Gently wash three times for 10 minutes.
. Add secondary antibody at the appropriate dilution. Incubate for 1 hour at room temperature.
. Remove antibody and replace with wash buffer. Gently wash three times for 10 minutes.
. Nuclei can be staining with 4',6' diamino phenylindole (DAPI) at 0.1 µg/ml, or coverslips can be directly mounted in media containing DAPI.
. Cells can now be viewed with a fluorescence microscope.*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow proper laboratory procedures for the disposal of formalin.
Restrictions For Research Use only
Format Liquid
Preservative Without preservative
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Aliquot and store at -20 °C or -80 °C.
Supplier Images
Immunofluorescence (IF) image for anti-Kinesin Heavy Chain Member 2A (KIF2A) (N-Term) antibody (ABIN268909) Staining of HeLa cells fixed in 3.5% paraformaldehyde using ABIN152994 (1:5,000). Ce...
Product cited in: Jang, Wong, Coppinger et al.: "DDA3 recruits microtubule depolymerase Kif2a to spindle poles and controls spindle dynamics and mitotic chromosome movement." in: The Journal of cell biology, Vol. 181, Issue 2, pp. 255-67, 2008 (PubMed).

Ferenz, Wadsworth: "Prophase microtubule arrays undergo flux-like behavior in mammalian cells." in: Molecular biology of the cell, Vol. 18, Issue 10, pp. 3993-4002, 2007 (PubMed).

Ganem, Compton: "The KinI kinesin Kif2a is required for bipolar spindle assembly through a functional relationship with MCAK." in: The Journal of cell biology, Vol. 166, Issue 4, pp. 473-8, 2004 (PubMed).

Homma, Takei, Tanaka et al.: "Kinesin superfamily protein 2A (KIF2A) functions in suppression of collateral branch extension." in: Cell, Vol. 114, Issue 2, pp. 229-39, 2003 (PubMed).