ICOS antibody

Catalog No. ABIN2689138

Product Details anti-ICOS Antibody

Target: ICOS (Inducible T-Cell Co-Stimulator (ICOS))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ICOS antibody is un-conjugated

Application: Flow Cytometry (FACS)

Brand: BD Pharmingen™

Characteristics: Monoclonal antibody DX29 reacts with inducible costimulatory (ICOS) molecule. ICOS is a homodimeric membrane glycoprotein, member of the CD28 family, of approximately 50 - 60 kDa, highly expressed on activated T cells. It is the receptor for B7 related protein 1 (B7RP-1) and also, like CD28, ICOS is costimulatory signal for T cell activation, proliferation and cytokine production. It is not expressed on resting or activated B cells, monocytes, NK cells, granulocytes, dendritic cells or platelets. Unlike the constitutively expressed CD28, ICOS expression is de novo. Reports describe similarities of CD28 and ICOS in T cell activation, however, it has been suggested that ICOS may play an important role in IL-10 production. In presence IL-10, purified recombinant human ICOS significantly increased in vitro B cell growth stimulated by pokeweed mitogen (PWM) and enhanced production of IgG. Profile of ICOS (DX29) reactivity on PHA-stimulated peripheral blood mononuclear cells analyzed by flow cytometry. Second step staining with Cat. No. 555988.

BD Pharmingen™ Purified Mouse Anti-Human CD278 - Purified - Clone DX29 - Isotype Mouse IgG1, κ - Reactivity Hu - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Activated human T cells

Clone: DX29

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-ICOS antibody (ABIN2689138)

Dong, Nurieva: "Regulation of immune and autoimmune responses by ICOS." in: Journal of autoimmunity, Vol. 21, Issue 3, pp. 255-60, (2003) (PubMed).

Okamoto, Tezuka, Kato, Abe, Tsuji: "PI3-kinase and MAP-kinase signaling cascades in AILIM/ICOS- and CD28-costimulated T-cells have distinct functions between cell proliferation and IL-10 production." in: Biochemical and biophysical research communications, Vol. 310, Issue 3, pp. 691-702, (2003) (PubMed).

Sakamoto, Tezuka, Tsuji, Hori, Tamatani: "AILIM/ICOS: its expression and functional analysis with monoclonal antibodies." in: Hybridoma and hybridomics, Vol. 20, Issue 5-6, pp. 293-303, (2002) (PubMed).

Aicher, Hayden-Ledbetter, Brady, Pezzutto, Richter, Magaletti, Buckwalter, Ledbetter, Clark: "Characterization of human inducible costimulator ligand expression and function." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 9, pp. 4689-96, (2000) (PubMed).

Target Details for ICOS

Target: ICOS (Inducible T-Cell Co-Stimulator (ICOS))

Alternative Name: CD278 (ICOS Products)

Synonyms: ICOS antibody, AILIM antibody, CCLP antibody, CRP-1 antibody, H4 antibody, Ly115 antibody, CD278 antibody, CVID1 antibody, Ailim antibody, inducible T-cell costimulator antibody, inducible T cell co-stimulator antibody, inducible T-cell co-stimulator antibody, ICOS antibody, LOC100232284 antibody, Icos antibody

Background: Synonyms: ICOS

Pathways: Cancer Immune Checkpoints