Baculoviral IAP Repeat-Containing 5 (BIRC5) antibody (HRP)

Details for Product No. ABIN268915
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Antigen
Synonyms API4, EPR-1, AAC-11, Api4, TIAP, survivin40, AP14
Reactivity
Dog (Canine)
(66), (25), (8), (1), (1), (1), (1)
Host
Rabbit
(68), (14), (2)
Clonality
Polyclonal
Conjugate
HRP
Application
Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
(68), (25), (24), (17), (17), (12), (7), (4), (4), (3), (1), (1), (1)
Pubmed 14 references available
Quantity 0.05 mL
Shipping to United States (Change)
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Catalog No. ABIN268915
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Immunogen Full length recombinant human Survivin
Purification affinity purified
Alternative Name IAP4 / BIRC5
Background Regulated inhibition of programmed cell death (apoptosis) preserves normalhomeostasis and tissue and organ morphogenesis. Aberrations in this processcontribute to human diseases and cancer by abnormally prolonging cell viability. Recently, several apoptosis inhibitors related to the baculovirus iap gene have beenfound in various species, including human. IAP proteins contain one/three Cys/Hisbaculovirus IAP repeats plus a C-terminal RING finger and are thought to block anevolutionary conserved step in apoptosis.Survivin encodes a structurally unique inhibitor of apoptosis (IAP). Survivin expressionis turned off during fetal development and is not found in non-neoplastic adult humantissues. Survivin becomes abundantly re-expressed in transformed cells and in all ofthe most common cancers of lung, colon, pancreas, breast and prostate in vivo. Survivin appears to be situated at the crossroads of cell death and cell division,governing a checkpoint involved in cytokinesis while also suppressing apoptosis. Survivin is also abundantly expressed in brain tissues (astrocytes and some neurons) ofadult rats following traumatic brain injury. Survivin has been found co-expressed withNeuN (mature neuronal marker) and PCNA (a cell cycle protein). Survivin might affectregulation of neural cell proliferative responses after brain injury. Alternate Names: anti-API4 antibody, anti-Apoptosis Inhibitor 4 antibody, anti-Apoptosis inhibitor survivinantibody, anti-Baculoviral IAP repeat containing 5 antibody, anti-BIRC 5 antibody,anti-EPR 1 antibody, anti-IAP4 antibody, anti-SVV antibody, anti-TIAP antibody,anti-BIRC5 antibody.
Gene Symbol: BIRC5
Gene ID 332, 11799, 64041
UniProt O15392
Research Area Cancer, Apoptosis/Necrosis, Neurology
Application Notes This Survivin antibody is useful for Chromatin Immunoprecipitation, Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections, Immunoprecipitation and Western Blot. In WB, a band at ~16.5 kDa can be seen. For IHC, prior antigen retrieval (pressure cooking) is recommended for cytoplasmic and nuclear detection of Survivin. Immunohistochemistry-Frozen and Flow Cytometry were reported in scientific literature.
Recommended dilutions: Immunocytochemistry/Immunofluorescence 1:50-1:250, Immunohistochemistry 0.5 µg/mL, Immunohistochemistry-Paraffin 0.5 µg/mL, Immunoprecipitation 1:10-1:500, Western Blot 1 µg/mL
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Preservative Without preservative
Storage 4 °C
Storage Comment 4 °C in the dark
General Wheatley, Carvalho, Vagnarelli et al.: "INCENP is required for proper targeting of Survivin to the centromeres and the anaphase spindle during mitosis." in: Current biology : CB, Vol. 11, Issue 11, pp. 886-90, 2001 (PubMed).

Zhu, Fukuda, Cordis et al.: "Anti-apoptotic protein survivin plays a significant role in tubular morphogenesis of human coronary arteriolar endothelial cells by hypoxic preconditioning." in: FEBS letters, Vol. 508, Issue 3, pp. 369-74, 2001 (PubMed).

Zeitlin, Shelby, Sullivan: "CENP-A is phosphorylated by Aurora B kinase and plays an unexpected role in completion of cytokinesis." in: The Journal of cell biology, Vol. 155, Issue 7, pp. 1147-57, 2001 (PubMed).

Chakravarti, Noll, Black et al.: "Quantitatively determined survivin expression levels are of prognostic value in human gliomas." in: Journal of clinical oncology : official journal of the American Society of Clinical Oncology, Vol. 20, Issue 4, pp. 1063-8, 2002 (PubMed).

Fortugno, Wall, Giodini et al.: "Survivin exists in immunochemically distinct subcellular pools and is involved in spindle microtubule function." in: Journal of cell science, Vol. 115, Issue Pt 3, pp. 575-85, 2002 (PubMed).

Giodini, Kallio, Wall et al.: "Regulation of microtubule stability and mitotic progression by survivin." in: Cancer research, Vol. 62, Issue 9, pp. 2462-7, 2002 (PubMed).

Zhang, Hazarika, Ni et al.: "Induction of apoptosis by bexarotene in cutaneous T-cell lymphoma cells: relevance to mechanism of therapeutic action." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 8, Issue 5, pp. 1234-40, 2002 (PubMed).

Yu, Leung, Ebert et al.: "Increased expression of survivin in gastric cancer patients and in first degree relatives." in: British journal of cancer, Vol. 87, Issue 1, pp. 91-7, 2002 (PubMed).

Blanc-Brude, Yu, Simosa et al.: "Inhibitor of apoptosis protein survivin regulates vascular injury." in: Nature medicine, Vol. 8, Issue 9, pp. 987-94, 2002 (PubMed).

Mahotka, Liebmann, Wenzel et al.: "Differential subcellular localization of functionally divergent survivin splice variants." in: Cell death and differentiation, Vol. 9, Issue 12, pp. 1334-42, 2002 (PubMed).

Grabowski, Kuehnel, Muehr-Wilkenshoff et al.: "Prognostic value of nuclear survivin expression in oesophageal squamous cell carcinoma." in: British journal of cancer, Vol. 88, Issue 1, pp. 115-9, 2003 (PubMed).

Miranda, Dyer, Grandis et al.: "Differential activation of apoptosis regulatory pathways during monocytic vs granulocytic differentiation: a requirement for Bcl-X(L)and XIAP in the prolonged survival of monocytic cells." in: Leukemia, Vol. 17, Issue 2, pp. 390-400, 2003 (PubMed).

Kennedy, ODriscoll, Purcell et al.: "Prognostic importance of survivin in breast cancer." in: British journal of cancer, Vol. 88, Issue 7, pp. 1077-83, 2003 (PubMed).

Hauf, Cole, LaTerra et al.: "The small molecule Hesperadin reveals a role for Aurora B in correcting kinetochore-microtubule attachment and in maintaining the spindle assembly checkpoint." in: The Journal of cell biology, Vol. 161, Issue 2, pp. 281-94, 2003 (PubMed).

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