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BIRC5 antibody (Baculoviral IAP Repeat-Containing 5) (HRP)

Details for Product anti-BIRC5 Antibody No. ABIN268915, Supplier: Log in to see
  • AAC-11
  • AP14
  • API4
  • Api4
  • EPR-1
  • survivin40
  • TIAP
anti-Dog (Canine) BIRC5 antibody for Immunofluorescence
Dog (Canine)
This BIRC5 antibody is conjugated to HRP
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
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Immunogen Full length recombinant human Survivin
Purification affinity purified
Alternative Name IAP4 / BIRC5 (BIRC5 Antibody Abstract)
Background Regulated inhibition of programmed cell death (apoptosis) preserves normalhomeostasis and tissue and organ morphogenesis. Aberrations in this processcontribute to human diseases and cancer by abnormally prolonging cell viability. Recently, several apoptosis inhibitors related to the baculovirus iap gene have beenfound in various species, including human. IAP proteins contain one/three Cys/Hisbaculovirus IAP repeats plus a C-terminal RING finger and are thought to block anevolutionary conserved step in apoptosis.Survivin encodes a structurally unique inhibitor of apoptosis (IAP). Survivin expressionis turned off during fetal development and is not found in non-neoplastic adult humantissues. Survivin becomes abundantly re-expressed in transformed cells and in all ofthe most common cancers of lung, colon, pancreas, breast and prostate in vivo. Survivin appears to be situated at the crossroads of cell death and cell division,governing a checkpoint involved in cytokinesis while also suppressing apoptosis. Survivin is also abundantly expressed in brain tissues (astrocytes and some neurons) ofadult rats following traumatic brain injury. Survivin has been found co-expressed withNeuN (mature neuronal marker) and PCNA (a cell cycle protein). Survivin might affectregulation of neural cell proliferative responses after brain injury. Alternate Names: anti-API4 antibody, anti-Apoptosis Inhibitor 4 antibody, anti-Apoptosis inhibitor survivinantibody, anti-Baculoviral IAP repeat containing 5 antibody, anti-BIRC 5 antibody,anti-EPR 1 antibody, anti-IAP4 antibody, anti-SVV antibody, anti-TIAP antibody,anti-BIRC5 antibody.
Gene Symbol: BIRC5
Gene ID 332, 11799, 64041
UniProt O15392
Research Area Cancer, Apoptosis/Necrosis, Neurology
Pathways Apoptosis, Cell Division Cycle
Application Notes This Survivin antibody is useful for Chromatin Immunoprecipitation, Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections, Immunoprecipitation and Western Blot. In WB, a band at ~16.5 kDa can be seen. For IHC, prior antigen retrieval (pressure cooking) is recommended for cytoplasmic and nuclear detection of Survivin. Immunohistochemistry-Frozen and Flow Cytometry were reported in scientific literature.
Recommended dilutions: Immunocytochemistry/Immunofluorescence 1:50-1:250, Immunohistochemistry 0.5 µg/mL, Immunohistochemistry-Paraffin 0.5 µg/mL, Immunoprecipitation 1:10-1:500, Western Blot 1 µg/mL
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Preservative Without preservative
Storage 4 °C
Storage Comment 4 °C in the dark
Product cited in: Hauf, Cole, LaTerra, Zimmer, Schnapp, Walter, Heckel, van Meel, Rieder, Peters: "The small molecule Hesperadin reveals a role for Aurora B in correcting kinetochore-microtubule attachment and in maintaining the spindle assembly checkpoint." in: The Journal of cell biology, Vol. 161, Issue 2, pp. 281-94, 2003

Kennedy, ODriscoll, Purcell, Fitz-Simons, McDermott, Hill, OHiggins, Parkinson, Linehan, Clynes: "Prognostic importance of survivin in breast cancer." in: British journal of cancer, Vol. 88, Issue 7, pp. 1077-83, 2003

Grabowski, Kuehnel, Muehr-Wilkenshoff, Heine, Stein, Hoepfner, Germer, Scheruebl: "Prognostic value of nuclear survivin expression in oesophageal squamous cell carcinoma." in: British journal of cancer, Vol. 88, Issue 1, pp. 115-9, 2003

Miranda, Dyer, Grandis, Johnson: "Differential activation of apoptosis regulatory pathways during monocytic vs granulocytic differentiation: a requirement for Bcl-X(L)and XIAP in the prolonged survival of monocytic cells." in: Leukemia, Vol. 17, Issue 2, pp. 390-400, 2003

Blanc-Brude, Yu, Simosa, Conte, Sessa, Altieri: "Inhibitor of apoptosis protein survivin regulates vascular injury." in: Nature medicine, Vol. 8, Issue 9, pp. 987-94, 2002

Zhang, Hazarika, Ni, Weidner, Duvic: "Induction of apoptosis by bexarotene in cutaneous T-cell lymphoma cells: relevance to mechanism of therapeutic action." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 8, Issue 5, pp. 1234-40, 2002

Giodini, Kallio, Wall, Gorbsky, Tognin, Marchisio, Symons, Altieri: "Regulation of microtubule stability and mitotic progression by survivin." in: Cancer research, Vol. 62, Issue 9, pp. 2462-7, 2002

Yu, Leung, Ebert, Ng, Go, Wang, Chung, Malfertheiner, Sung: "Increased expression of survivin in gastric cancer patients and in first degree relatives." in: British journal of cancer, Vol. 87, Issue 1, pp. 91-7, 2002

Mahotka, Liebmann, Wenzel, Suschek, Schmitt, Gabbert, Gerharz: "Differential subcellular localization of functionally divergent survivin splice variants." in: Cell death and differentiation, Vol. 9, Issue 12, pp. 1334-42, 2002

Zhu, Fukuda, Cordis, Das, Maulik: "Anti-apoptotic protein survivin plays a significant role in tubular morphogenesis of human coronary arteriolar endothelial cells by hypoxic preconditioning." in: FEBS letters, Vol. 508, Issue 3, pp. 369-74, 2001

Zeitlin, Shelby, Sullivan: "CENP-A is phosphorylated by Aurora B kinase and plays an unexpected role in completion of cytokinesis." in: The Journal of cell biology, Vol. 155, Issue 7, pp. 1147-57, 2001

Background publications Chakravarti, Noll, Black, Finkelstein, Finkelstein, Dyson, Loeffler: "Quantitatively determined survivin expression levels are of prognostic value in human gliomas." in: Journal of clinical oncology : official journal of the American Society of Clinical Oncology, Vol. 20, Issue 4, pp. 1063-8, 2002

Fortugno, Wall, Giodini, OConnor, Plescia, Padgett, Tognin, Marchisio, Altieri: "Survivin exists in immunochemically distinct subcellular pools and is involved in spindle microtubule function." in: Journal of cell science, Vol. 115, Issue Pt 3, pp. 575-85, 2002

Wheatley, Carvalho, Vagnarelli, Earnshaw: "INCENP is required for proper targeting of Survivin to the centromeres and the anaphase spindle during mitosis." in: Current biology : CB, Vol. 11, Issue 11, pp. 886-90, 2001