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Caspase 7 antibody (Caspase 7, Apoptosis-Related Cysteine Peptidase)

Details for Product anti-CASP7 Antibody No. ABIN268919, Supplier: Log in to see
Antigen
  • AI314680
  • CASP-7
  • CASP7
  • Caspase-7
  • caspase-7
  • caspase7
  • cmh-1
  • CMH-1
  • ICE-IAP3
  • ice-lap3
  • ICE-LAP3
  • LICE2
  • mCASP-7
  • Mch3
  • mch3
  • MCH3
  • MCH3/SCA-2
  • SCA-2
  • xCaspase-7
Alternatives
anti-Human Caspase 7 antibody for Immunoprecipitation
Reactivity
Human, Mouse (Murine), Rat (Rattus)
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110
87
8
7
5
5
4
4
2
2
1
Host
Mouse
204
92
4
Clonality (Clone)
Monoclonal ()
Conjugate
This Caspase 7 antibody is un-conjugated
14
11
9
4
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Application
Simple Western (SimWes), Western Blotting (WB)
252
104
86
74
31
20
14
12
8
8
5
4
3
1
Supplier
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Immunogen Full-length recombinant human Caspase 7 [UniProt# P55210]
Clone Mch3 1-1-11
Isotype IgG1 kappa
Purification Ascites
Alternative Name Caspase-7 (CASP7 Antibody Abstract)
Background Gene Symbol: CASP7
Molecular Weight Theoretical MW: 35 kDa
Gene ID 840
UniProt P55210
Research Area Apoptosis/Necrosis
Pathways Apoptosis, Caspase Cascade in Apoptosis
Application Notes Western Blot 1:500-1:1000, Simple Western 1:500
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for Caspase 7 Antibody Procedure Guide for NB 500-206 Monoclonal anti-Caspase 7 Western Protocol
1) Prepare samples for a NuPAGE gel and heat them at 70?C for 10 minutes.
2) Run a 4-12 % NuPAGE Bis-Tris gel, loading 50-100 mg of whole cell extract per lane, for 45 minutes at 200 Volts.
) Soak transfer sponges and gel 15 minutes and nitrocellulose membrane 5 minutes prior to transfer.
) Transfer the proteins to the nitrocellulose membrane for 1 hour at 30 Volts, in 4 degrees C transfer buffer.
) Block the membrane in TBST + 5 % non-fat dry milk, for 1 hour at room temperature (RT), shaking gently.
) Rinse the membrane twice with TBST.
) Incubate the membrane in anti-Caspase 7 [NB 500-206], diluted 1:500-1:1,000 in TBS + 1 % BSA, for 90 minutes at RT.
) Wash the membrane once for 15 minutes, then 3 times 5 minutes, in TBST at RT.
) Incubate the membrane in anti-mouse conjugated to HRP (secondary antibody), diluted in TBS + 1 % BSA for 35 minutes at RT.
) Wash the membrane once for 15 minutes, then 3 times 5 minutes, in TBST at RT.
) Develop membrane in chemiluminescent reagents, as instructed by kit-vendor.*Positive control(s): HeLa whole cell extracts (cat# NB 800-PC1) Jurkat whole cell extracts (cat# NB 800-PC2)
Restrictions For Research Use only
Format Liquid
Buffer Buffer contains: 0.1 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Product cited in: Badr, Hafez, Agha, Shouman: "The Combination of α-Tocopheryl Succinate and Sodium Selenite on Breast Cancer: A Merit or a Demerit?" in: Oxidative medicine and cellular longevity, Vol. 2016, pp. 4741694, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting

Blanc-Brude, Mesri, Wall, Plescia, Dohi, Altieri: "Therapeutic targeting of the survivin pathway in cancer: initiation of mitochondrial apoptosis and suppression of tumor-associated angiogenesis." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 9, Issue 7, pp. 2683-92, 2003 (PubMed).

Background publications Kirsch, Doseff, Chau, Lim, de Souza-Pinto, Hansford, Kastan, Lazebnik, Hardwick: "Caspase-3-dependent cleavage of Bcl-2 promotes release of cytochrome c." in: The Journal of biological chemistry, Vol. 274, Issue 30, pp. 21155-61, 1999 (PubMed).

Fearnhead, Rodriguez, Govek, Guo, Kobayashi, Hannon, Lazebnik: "Oncogene-dependent apoptosis is mediated by caspase-9." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 23, pp. 13664-9, 1998 (PubMed).