NCR1 antibody

Catalog No. ABIN2689197

Product Details anti-NCR1 Antibody

Target: NCR1 (Natural Cytotoxicity Triggering Receptor 1 (NCR1))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This NCR1 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Brand: BD Pharmingen™

Characteristics: The monoclonal antibody 29A1.4 specifically binds to mouse CD335, also known as NKp46. NKp46 is a 46 kDa type I transmembrane glycoprotein that is a member of the natural cytotoxicity receptor (NCR) family and immunoglobulin superfamily. NKp46 is encoded by the Ncr1 gene located on chromosome 7. NKp46 functions as a cytotoxicity triggering receptor and is selectively expressed by immature and mature NK cells in all mouse strains tested. NKp46 is detected on a minute fraction of NK-like T cells (less than 2 % of NKp46+ express CD3e) but not on CD1d-restricted NKT cells from C57BL/6 mice. When immobilized on tissue culture plates, the 29A1.4 antibody reportedly stimulates NK cells to produce interferon-gamma and to release their cytoplasmic granule contents. Although the ligands for the NKp46 receptor have not been fully characterized, recent evidence indicates that this receptor plays an important role in the NK cell-mediated recognition and killing of some virus-infected cells and tumor cells. The immunogen used to generate the 29A1.4 clone was mouse NKp46-Fc recombinant protein. Flow cytometric analysis of CD335 (NKp46) expression on mouse splenocytes. C57BL/6 and BALB/c mouse spleen cells were stained separately with Purified anti-mouse CD335 (NKp46) antibody followed by PE-conjugated Goat anti-rat Ig (Cat. No. 550767). After washing, C57BL/6 cells were stained with APC-conjugated anti-mouse NK-1.1 (NKR-P1B and NKR-P1C) antibody (Cat. No.550627, left panel) and BALB/c cells were stained with FITC-conjugated anti-mouse CD49b (DX5) antibody (Cat. No.553857, right panel). Two-color dot plots showing the correlated expression patterns of CD335/NKp46 and either NK-1.1/CD161 (C57BL/6 cells, left panel) or DX5/CD49b (BALB/c cells, right panel) were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD™ LSRII System.

BD Pharmingen™ Purified Rat Anti-Mouse CD335 (NKp46) - Purified - Clone 29A1.4 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Clone: 29A1-4

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-NCR1 antibody (ABIN2689197)

Joncker, Fernandez, Treiner, Vivier, Raulet: "NK cell responsiveness is tuned commensurate with the number of inhibitory receptors for self-MHC class I: the rheostat model." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 182, Issue 8, pp. 4572-80, (2009) (PubMed).

Walzer, Bléry, Chaix, Fuseri, Chasson, Robbins, Jaeger, André, Gauthier, Daniel, Chemin, Morel, Dalod, Imbert, Pierres, Moretta, Romagné, Vivier: "Identification, activation, and selective in vivo ablation of mouse NK cells via NKp46." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 9, pp. 3384-9, (2007) (PubMed).

Gazit, Gruda, Elboim, Arnon, Katz, Achdout, Hanna, Qimron, Landau, Greenbaum, Zakay-Rones, Porgador, Mandelboim: "Lethal influenza infection in the absence of the natural killer cell receptor gene Ncr1." in: Nature immunology, Vol. 7, Issue 5, pp. 517-23, (2006) (PubMed).

Biassoni, Pessino, Bottino, Pende, Moretta, Moretta: "The murine homologue of the human NKp46, a triggering receptor involved in the induction of natural cytotoxicity." in: European journal of immunology, Vol. 29, Issue 3, pp. 1014-20, (1999) (PubMed).

Target Details for NCR1

Target: NCR1 (Natural Cytotoxicity Triggering Receptor 1 (NCR1))

Alternative Name: CD335 NKp46 (NCR1 Products)

Synonyms: CD335 antibody, LY94 antibody, NK-p46 antibody, NKP46 antibody, Ly94 antibody, NKp46 antibody, KILR-1 antibody, Nk-p46 antibody, Nkp46 antibody, natural cytotoxicity triggering receptor 1 antibody, NCR1 antibody, Ncr1 antibody

Background: Synonyms: NKp46, Ar1, Ly94, Lymphocyte antigen 94, Mar1, MAR-1, Mouse activating rece

Pathways: Regulation of Leukocyte Mediated Immunity