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CAF-1 Subunit antibody

Details for Product No. ABIN268920, Supplier: Log in to see
Clonality (Clone)
Monoclonal ()
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
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Immunogen Recombinant human CHAF1B
Clone SS 53
Isotype IgG1
Purification Ascites
Background Chromatin assembly factor 1 (CAF-1) is a member of the histone chaperone family. It isrequired for inheritance of epigenetically determined chromosomal states, and in vitro, itpromotes the assembly of chromatin during DNA replication and DNA repair. CAF-1 isa complex of three subunits, p150, p60, and p48. This complex is localized to thenucleus of human cells.Both the composition and the biochemical activity of CAF-1 are conserved amonghuman, yeast, and Drosophila. Alternate Names: anti-p60 CAF1 antibody.
Gene Symbol: CHAF1B
Gene ID 8208
UniProt Q13112
Application Notes This CHAF1B (SS 53) antibody is useful in Western blot and Immunoprecipitation. Immunocytochemistry/Immunofluorescence was reported in scientific literature. In WB a band can be seen at ~60 kDa.
Recommended dilutions: Immunocytochemistry/Immunofluorescence, Immunoprecipitation 1:10-1:500, Western Blot 1:100-1:2000
Restrictions For Research Use only
Format Liquid
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Aliquot and store at -20 °C or -80 °C.
Product cited in: Galvani, Courbeyrette, Agez et al.: "In vivo study of the nucleosome assembly functions of ASF1 histone chaperones in human cells." in: Molecular and cellular biology, Vol. 28, Issue 11, pp. 3672-85, 2008 (PubMed).

Green, Almouzni: "Local action of the chromatin assembly factor CAF-1 at sites of nucleotide excision repair in vivo." in: The EMBO journal, Vol. 22, Issue 19, pp. 5163-74, 2003 (PubMed).

Background publications Shibahara, Stillman: "Replication-dependent marking of DNA by PCNA facilitates CAF-1-coupled inheritance of chromatin." in: Cell, Vol. 96, Issue 4, pp. 575-85, 1999 (PubMed).

Smith, Stillman: "Immunological characterization of chromatin assembly factor I, a human cell factor required for chromatin assembly during DNA replication in vitro." in: The Journal of biological chemistry, Vol. 266, Issue 18, pp. 12041-7, 1991 (PubMed).