CAF-1 Subunit antibody

Antigen: CAF-1 Subunit

Clonality: Monoclonal (SS 53)

Host: Mouse

Reactivity: Human

Application: Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN268920

Additional Information

Gene ID: 8208

Immunogen: Recombinant human CAF-1p60.

Cross-Reactivity: Human

Format: Ascites

Isotype: IgG1

Clone: SS 53

Description: Chromatin assembly factor 1 (CAF-1) is a member of the histone chaperone family. It isrequired for inheritance of epigenetically determined chromosomal states, and in vitro, itpromotes the assembly of chromatin during DNA replication and DNA repair. CAF-1 isa complex of three subunits, p150, p60, and p48. This complex is localized to thenucleus of human cells.Both the composition and the biochemical activity of CAF-1 are conserved amonghuman, yeast, and Drosophila. Alternate Names: anti-p60 CAF1 antibody.

Specificity: Specific for CAF-1p60. Species Reactivity: Recognizes human CAF-1p60. Other species have not been tested.

Application Details

Application Notes: Western analysis: Use a 1:1,000 dilution (ECL)on crude nuclear extracts. A band isseen at 60 kDa representing CAF-1p60. In addition, this antibody may be used forimmunoprecipitation (100-500 ng of IgG).

Purification: Ascites

Buffer: Ascitic fluid containing 0.1% sodium azide

Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Restrictions: For Research Use only

Publications

Smith, Stillman: "Immunological characterization of chromatin assembly factor I, a human cell factor required for chromatin assembly during DNA replication in vitro." in: The Journal of biological chemistry, Vol. 266, Issue 18, pp. 12041-7, 1991 (PubMed).

Shibahara, Stillman: "Replication-dependent marking of DNA by PCNA facilitates CAF-1-coupled inheritance of chromatin." in: Cell, Vol. 96, Issue 4, pp. 575-85, 1999 (PubMed).

Green, Almouzni: "Local action of the chromatin assembly factor CAF-1 at sites of nucleotide excision repair in vivo." in: The EMBO journal, Vol. 22, Issue 19, pp. 5163-74, 2003 (PubMed).

Galvani, Courbeyrette, Agez et al.: "In vivo study of the nucleosome assembly functions of ASF1 histone chaperones in human cells." in: Molecular and cellular biology, Vol. 28, Issue 11, pp. 3672-85, 2008 (PubMed).