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Survivin antibody (BIRC5) (Nuclear)

Details for Product anti-BIRC5 Antibody No. ABIN268921, Supplier: Log in to see
Antigen
  • AAC-11
  • api4
  • Api4
  • BIR1
  • bir5a
  • birc5
  • BIRC5
  • epr-1
  • LOC398389
  • SIX
  • Su1
  • Su2
  • Survivin
  • survivin
  • Survivin1
  • survivin40
  • svv1
  • svv2
  • TIAP
  • XBIR1
  • xsurv
  • xSurvivin
  • xSurvivin1
  • xSurvivin2
Epitope
Nuclear
68
18
15
13
13
12
6
6
6
5
5
4
4
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Hamster, Human
275
92
74
7
7
1
1
1
Host
Mouse
215
66
15
1
Clonality (Clone)
Monoclonal ()
Conjugate
This Survivin antibody is un-conjugated
14
8
8
5
5
5
3
2
2
2
2
2
2
2
2
2
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
215
103
64
62
60
48
22
20
11
9
5
3
2
2
2
2
2
1
Supplier
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Immunogen Full length recombinant human Survivin [UniProt# O15392]
Clone 32-1
Isotype IgG1 kappa
Specificity This antibody is specific for the nuclear form of survivin.
Purification Protein G purified
Alternative Name Survivin (BIRC5 Antibody Abstract)
Background Gene Symbol: BIRC5
Molecular Weight Theoretical MW: 16.5 kDa
UniProt O15392
Research Area Neurology, Apoptosis/Necrosis, Glia marker, Cancer
Application Notes Western Blot 1:1000, ELISA, Immunohistochemistry 20 μg/mL, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunoprecipitation, Immunohistochemistry-Paraffin 20 μg/mL
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for Survivin Antibody IHC-FFPE sections I. Deparaffinization:A. Treat slides with Xylene: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes. B. Treat slides with 100 % Reagent Alcohol: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes. II. Quench Endogenous Peroxidase: A. Place slides in peroxidase quenching solution: 15-30 minutes. To Prepare 200 mL of Quenching Solution: Add 3 mL of 30 % Hydrogen Peroxide to 200 mL of Methanol. Use within 4 hours of preparation B. Place slides in distilled water: 2 changes for 2 minutes each. III. Retrieve Epitopes: A. Preheat Citrate
Restrictions For Research Use only
Format Liquid
Concentration 1.9 mg/mL
Buffer PBS
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C,-80 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Supplier Images
Immunohistochemistry (IHC) image for anti-Survivin antibody (BIRC5) (Nuclear) (ABIN268921) Staining of human lung, cancer using ABIN153037.
Product cited in: Giodini, Kallio, Wall, Gorbsky, Tognin, Marchisio, Symons, Altieri: "Regulation of microtubule stability and mitotic progression by survivin." in: Cancer research, Vol. 62, Issue 9, pp. 2462-7, 2002

Adida, Haioun, Gaulard, Lepage, Morel, Briere, Dombret, Reyes, Diebold, Gisselbrecht, Salles, Altieri, Molina: "Prognostic significance of survivin expression in diffuse large B-cell lymphomas." in: Blood, Vol. 96, Issue 5, pp. 1921-5, 2000

Grossman, McNiff, Li, Altieri: "Expression and targeting of the apoptosis inhibitor, survivin, in human melanoma." in: The Journal of investigative dermatology, Vol. 113, Issue 6, pp. 1076-81, 2000

Li, Ackermann, Bennett, Rothermel, Plescia, Tognin, Villa, Marchisio, Altieri: "Pleiotropic cell-division defects and apoptosis induced by interference with survivin function." in: Nature cell biology, Vol. 1, Issue 8, pp. 461-6, 2000

Background publications Fortugno, Wall, Giodini, OConnor, Plescia, Padgett, Tognin, Marchisio, Altieri: "Survivin exists in immunochemically distinct subcellular pools and is involved in spindle microtubule function." in: Journal of cell science, Vol. 115, Issue Pt 3, pp. 575-85, 2002

Li, Ambrosini, Chu, Plescia, Tognin, Marchisio, Altieri: "Control of apoptosis and mitotic spindle checkpoint by survivin." in: Nature, Vol. 396, Issue 6711, pp. 580-4, 1999

Ambrosini, Adida, Sirugo, Altieri: "Induction of apoptosis and inhibition of cell proliferation by survivin gene targeting." in: The Journal of biological chemistry, Vol. 273, Issue 18, pp. 11177-82, 1998