CD4 antibody (CD4 Molecule)

Details for Product anti-CD4 Antibody No. ABIN2689228, Supplier: Log in to see
Antigen
  • cd4
  • L3T4
  • Ly-4
  • CD4mut
  • W3/25
  • p55
  • CD4 molecule
  • CD4 antigen
  • T-cell surface glycoprotein CD4
  • Cd4 molecule
  • CD4
  • cd4
  • Cd4
Alternatives
anti-Human CD4 antibody for Flow Cytometry
Reactivity
Rat (Rattus)
1220
632
233
68
67
40
36
27
26
22
21
19
18
16
15
15
13
10
10
9
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6
5
5
5
4
4
3
3
3
3
2
2
2
2
2
1
1
1
1
Host
Mouse
1376
587
281
11
4
1
1
1
Clonality (Clone)
Monoclonal ()
Conjugate
This CD4 antibody is un-conjugated
266
227
170
103
49
48
46
40
36
34
34
30
27
25
21
21
19
19
18
18
18
18
16
15
15
13
13
13
11
9
9
8
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5
5
4
4
4
4
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3
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Blocking Reagent (BR), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunoprecipitation (IP)
1743
490
445
352
323
308
157
155
88
78
39
38
31
30
29
25
18
14
14
11
9
9
6
6
5
4
4
3
3
2
2
2
2
2
2
1
1
1
1
1
1
Options
Supplier
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Supplier Product No.
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Brand BD Pharmingen™
Immunogen Rat thymocyte glycoproteins
Clone OX
Isotype IgG2a, kappa
Characteristics The OX-38 antibody has been reported to react with the CD4 antigen on most thymocytes, a subpopulation of mature T lymphocytes (i.e., MHC class II-restricted T cells, including most T helper cells), monocytes, macrophages, and some dendritic cells. CD4 is an antigen coreceptor on the T-cell surface which interacts with MHC class II molecules on antigen-presenting cells. It participates in T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase lck. The OX-38 antibody has been reported to bind to the same epitope of CD4 as that recognized by W3/25 mAb, which is a different epitope than that recognized by OX-35 mAb (Cat. No. 554837). In vivo blocking of some cell-mediated immune responses by mAb OX-38 has been reported. Injection of OX-38 mAb induces allograft unresponsiveness in rats, with varying results depending on the rat strain used (high or low responder). Furthermore, in vivo depletion of CD4+ lymphocytes has been reported with this antibody. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Two-color analysis of the expression of CD4 on rat splenocytes. Lewis splenocytes were incubated simultaneously with PE-conjugated anti-rat CD3 G4.18 (Cat. No. 554833) and purified OX-38 (right panel), followed by FITC anti-mouse IgG2a R19-15 (Cat. No. 553390) monoclonal antibodies. The CD3-negative CD4-dim cells are the monocyte/macrophage population. Flow cytometry was performed on a BD FACScan™ flow cytometry system.

BD Pharmingen™ Purified Mouse Anti-Rat CD4 - Purified - Clone OX-38 - Isotype Mouse IgG2a, κ - Reactivity Rat - 0.5 mg
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name CD4 (CD4 Antibody Abstract)
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.
Product cited in: Liu, Zhang, Jenkins, MacPherson: "Dendritic cell heterogeneity in vivo: two functionally different dendritic cell populations in rat intestinal lymph can be distinguished by CD4 expression." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 3, pp. 1146-55, 1998 (PubMed).

Suzuki, Hara, Miyahara, Tanigawa, Matsuzaki, Onitsuka, Shibata, Koga, Yamashita, Miyamoto, Suzuki, Amemiya, Iwaya, Miyasaka, Kimura: "Microchimerism and graft acceptance: IV. Cardiac allograft acceptance following anti-adhesion molecule antibody therapy." in: Transplantation proceedings, Vol. 28, Issue 4, pp. 2058-60, 1996 (PubMed).

Yin, Fathman: "Tissue-specific effects of anti-CD4 therapy in induction of allograft unresponsiveness in high and low responder rats." in: Transplant immunology, Vol. 3, Issue 3, pp. 258-64, 1996 (PubMed).

Arima, Goss, Walp, Flye: "Administration of anti-CD4 monoclonal antibody with intrathymic injection of alloantigen results in rat cardiac allograft tolerance." in: Surgery, Vol. 118, Issue 2, pp. 265-72; discussion 272-3, 1995 (PubMed).

Bañuls, Alvarez, Ferrero, Zapata, Ardavin: "Cell-surface marker analysis of rat thymic dendritic cells." in: Immunology, Vol. 79, Issue 2, pp. 298-304, 1993 (PubMed).

Janeway: "The T cell receptor as a multicomponent signalling machine: CD4/CD8 coreceptors and CD45 in T cell activation." in: Annual review of immunology, Vol. 10, pp. 645-74, 1992 (PubMed).

Stitz, Sobbe, Bilzer: "Preventive effects of early anti-CD4 or anti-CD8 treatment on Borna disease in rats." in: Journal of virology, Vol. 66, Issue 6, pp. 3316-23, 1992 (PubMed).

Bierer, Sleckman, Ratnofsky, Burakoff: "The biologic roles of CD2, CD4, and CD8 in T-cell activation." in: Annual review of immunology, Vol. 7, pp. 579-99, 1989 (PubMed).

Jefferies, Green, Williams: "Authentic T helper CD4 (W3/25) antigen on rat peritoneal macrophages." in: The Journal of experimental medicine, Vol. 162, Issue 1, pp. 117-27, 1985 (PubMed).