CD4 antibody

Catalog No. ABIN2689228

Product Details anti-CD4 Antibody

Target: CD4

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD4 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Blocking Reagent (BR)

Brand: BD Pharmingen™

Characteristics: The OX-38 antibody has been reported to react with the CD4 antigen on most thymocytes, a subpopulation of mature T lymphocytes (i.e., MHC class II-restricted T cells, including most T helper cells), monocytes, macrophages, and some dendritic cells. CD4 is an antigen coreceptor on the T-cell surface which interacts with MHC class II molecules on antigen-presenting cells. It participates in T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase lck. The OX-38 antibody has been reported to bind to the same epitope of CD4 as that recognized by W3/25 mAb, which is a different epitope than that recognized by OX-35 mAb (Cat. No. 554837). In vivo blocking of some cell-mediated immune responses by mAb OX-38 has been reported. Injection of OX-38 mAb induces allograft unresponsiveness in rats, with varying results depending on the rat strain used (high or low responder). Furthermore, in vivo depletion of CD4+ lymphocytes has been reported with this antibody. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Two-color analysis of the expression of CD4 on rat splenocytes. Lewis splenocytes were incubated simultaneously with PE-conjugated anti-rat CD3 G4.18 (Cat. No. 554833) and purified OX-38 (right panel), followed by FITC anti-mouse IgG2a R19-15 (Cat. No. 553390) monoclonal antibodies. The CD3-negative CD4-dim cells are the monocyte/macrophage population. Flow cytometry was performed on a BD FACScan™ flow cytometry system.

BD Pharmingen™ Purified Mouse Anti-Rat CD4 - Purified - Clone OX-38 - Isotype Mouse IgG2a, κ - Reactivity Rat - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Rat thymocyte glycoproteins

Clone: OX

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-CD4 antibody (ABIN2689228)

Liu, Zhang, Jenkins, MacPherson: "Dendritic cell heterogeneity in vivo: two functionally different dendritic cell populations in rat intestinal lymph can be distinguished by CD4 expression." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 3, pp. 1146-55, (1998) (PubMed).

Suzuki, Hara, Miyahara, Tanigawa, Matsuzaki, Onitsuka, Shibata, Koga, Yamashita, Miyamoto, Suzuki, Amemiya, Iwaya, Miyasaka, Kimura: "Microchimerism and graft acceptance: IV. Cardiac allograft acceptance following anti-adhesion molecule antibody therapy." in: Transplantation proceedings, Vol. 28, Issue 4, pp. 2058-60, (1996) (PubMed).

Yin, Fathman: "Tissue-specific effects of anti-CD4 therapy in induction of allograft unresponsiveness in high and low responder rats." in: Transplant immunology, Vol. 3, Issue 3, pp. 258-64, (1996) (PubMed).

Arima, Goss, Walp, Flye: "Administration of anti-CD4 monoclonal antibody with intrathymic injection of alloantigen results in rat cardiac allograft tolerance." in: Surgery, Vol. 118, Issue 2, pp. 265-72; discussion 272-3, (1995) (PubMed).

Bañuls, Alvarez, Ferrero, Zapata, Ardavin: "Cell-surface marker analysis of rat thymic dendritic cells." in: Immunology, Vol. 79, Issue 2, pp. 298-304, (1993) (PubMed).

Janeway: "The T cell receptor as a multicomponent signalling machine: CD4/CD8 coreceptors and CD45 in T cell activation." in: Annual review of immunology, Vol. 10, pp. 645-74, (1992) (PubMed).

Stitz, Sobbe, Bilzer: "Preventive effects of early anti-CD4 or anti-CD8 treatment on Borna disease in rats." in: Journal of virology, Vol. 66, Issue 6, pp. 3316-23, (1992) (PubMed).

Bierer, Sleckman, Ratnofsky, Burakoff: "The biologic roles of CD2, CD4, and CD8 in T-cell activation." in: Annual review of immunology, Vol. 7, pp. 579-99, (1989) (PubMed).

Jefferies, Green, Williams: "Authentic T helper CD4 (W3/25) antigen on rat peritoneal macrophages." in: The Journal of experimental medicine, Vol. 162, Issue 1, pp. 117-27, (1985) (PubMed).

Target Details for CD4

Target: CD4

Alternative Name: CD4 (CD4 Products)

Synonyms: cd4 antibody, L3T4 antibody, Ly-4 antibody, CD4mut antibody, W3/25 antibody, p55 antibody, CD4 molecule antibody, carcinoembryonic antigen-related cell adhesion molecule 5 antibody, T-cell surface glycoprotein CD4 antibody, CD4 antigen antibody, Cd4 molecule antibody, CD4 antibody, LOC100125525 antibody, cd4 antibody, Cd4 antibody

Pathways: TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events