The MWReg30 antibody reacts with glycoprotein (gp) IIb, also called integrin αIIb chain (CD41), that associates with integrin β3 chain (gpIIIa or CD61) to form the gpIIb/IIIa (CD41/CD61) complex. CD41/CD61 is expressed on platelets, megakaryocytes, and early hematopoietic progenitors. The integrin complex binds to fibrinogen, fibronectin, vitronectin, von Willebrand factor, and thrombospondin. It is important for platelet adhesion and aggregation, and it may play a role in osteolytic tumor metastasis. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature.
BD Pharmingen™ Purified Rat Anti-Mouse CD41 - Purified - Clone MWReg30 - Isotype Rat IgG1, κ - Reactivity Ms - 0.5 mg
Purification
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C
Storage Comment
Store undiluted at 4°C.
Bakewell, Nestor, Prasad, Tomasson, Dowland, Mehrotra, Scarborough, Kanter, Abe, Phillips, Weilbaecher: "Platelet and osteoclast beta3 integrins are critical for bone metastasis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 24, pp. 14205-10, (2003) (PubMed).
Ferkowicz, Starr, Xie, Li, Johnson, Shelley, Morrison, Yoder: "CD41 expression defines the onset of primitive and definitive hematopoiesis in the murine embryo." in: Development (Cambridge, England), Vol. 130, Issue 18, pp. 4393-403, (2003) (PubMed).
Nieswandt, Echtenacher, Wachs, Schröder, Gessner, Schmidt, Grau, Männel: "Acute systemic reaction and lung alterations induced by an antiplatelet integrin gpIIb/IIIa antibody in mice." in: Blood, Vol. 94, Issue 2, pp. 684-93, (1999) (PubMed).
Kieffer, Phillips: "Platelet membrane glycoproteins: functions in cellular interactions." in: Annual review of cell biology, Vol. 6, pp. 329-57, (1991) (PubMed).
Phillips, Charo, Scarborough: "GPIIb-IIIa: the responsive integrin." in: Cell, Vol. 65, Issue 3, pp. 359-62, (1991) (PubMed).