Beclin 1, Autophagy Related (BECN1) antibody

Antigen: Beclin 1, Autophagy Related (BECN1)

Synonyms: ATG6, VPS30, beclin1, MGC6843, 4921513J16Rik, 5430417M23Rik, MGC73097, zgc:73097, BECN1, becn1, MGC80672, MGC126978, atg6, vps30, Beclin-1, DKFZp459C0719

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN268927

Additional Information

Alternative name: Beclin-1

Gene ID: 8678

Immunogen: ABIN153048 was raised against an internal synthetic peptide to human Beclin 1,containing residues within 1-100. This sequence has 94% identity with the mousesequence and 88% identity with the rat sequence.

Cross-Reactivity: Human, Mouse (Murine)

Description: Beclin 1 is the first identified mammalian gene to mediate autophagy and also has tumorsuppressor and antiviral function. Autophagy, a process of bulk protein degradationthrough an autophagosomic-lysosomal pathway, is important for differentiation, survivalduring nutrient deprivation, and normal growth control, and is often defective in tumorcells. Beclin 1 was originally isolated in a yeast two hybrid screen to identifyBcl-2-binding partners and maps to a tumor susceptibility locus on human chromosome17q21 that is frequently monoallelically deleted in human breast, ovarian and prostatecancer. Beclin 1 encodes an evolutionarily conserved 60 kDa coiled coil protein that isexpressed in human muscle, epithelial cells and neurons. In gene transfer studies,beclin 1 promotes nutrient deprivation-induced autophagy, inhibits mammarytumorigenesis, and inhibits viral replication. Expression of the Beclin 1 protein isfrequently decreased in malignant breast epithelial cells. Based upon theseobservations, it is speculated that beclin 1 may work through induction of autophagy tonegatively regulate tumorigenesis and to control viral infections. Beclin 1 may also playa role in other biologic processes in which autophagy is important such as celldifferentiation and nutritional stress responses. Alternate Names: anti-CTIP-1 COUP-TF interacting protein 1 antibody, anti-Beclin1 autophagy antibody,anti-Coiled-coil myosin-like BCL2-interacting protein antibody, anti-Protein GT197antibody, anti-APG6. GENE NAME: BECN1 antibody.

Specificity: This antibody is specific to human Beclin 1. Species Reactivity: This antibody can be used for detection of Beclin 1 in human samples. Preliminaryresults suggest that this antibody may cross-react with mouse protein, as well. Otherspecies have not been tested. Localization: Cell Membrane, Cytoplasmic and Golgi Apparatus. Expressed in dendrites and cellbodies of cerebellar Purkinje cells.

Application Details

Application Notes: Suggested working dilutions: Western Blot 1:2000-1:5000,Immunohistochemistry-Paraffin

Concentration: 1 mg/ml

Buffer: Tris-citrate/phosphate, pH 7-8. Preservative: 0.1% sodium azide.

Storage: Store at 4C. Do not freeze.

Restrictions: For Research Use only

Publications

Liang, Kleeman, Jiang et al.: "Protection against fatal Sindbis virus encephalitis by beclin, a novel Bcl-2-interacting protein." in: Journal of virology, Vol. 72, Issue 11, pp. 8586-96, 1998 (PubMed).

Aita, Liang, Murty et al.: "Cloning and genomic organization of beclin 1, a candidate tumor suppressor gene on chromosome 17q21." in: Genomics, Vol. 59, Issue 1, pp. 59-65, 1999 (PubMed).

Liang, Jackson, Seaman et al.: "Induction of autophagy and inhibition of tumorigenesis by beclin 1." in: Nature, Vol. 402, Issue 6762, pp. 672-6, 2000 (PubMed).

Massey, Kaushik, Sovak et al.: "Consequences of the selective blockage of chaperone-mediated autophagy." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 15, pp. 5805-10, 2006 (PubMed).

Park, Lee, Kim et al.: "A human scFv antibody against TRAIL receptor 2 induces autophagic cell death in both TRAIL-sensitive and TRAIL-resistant cancer cells." in: Cancer research, Vol. 67, Issue 15, pp. 7327-34, 2007 (PubMed).

Wang, Singh, Massey et al.: "Loss of macroautophagy promotes or prevents fibroblast apoptosis depending on the death stimulus." in: The Journal of biological chemistry, Vol. 283, Issue 8, pp. 4766-77, 2008 (PubMed).

Massey, Follenzi, Kiffin et al.: "Early cellular changes after blockage of chaperone-mediated autophagy." in: Autophagy, Vol. 4, Issue 4, pp. 442-56, 2008 (PubMed).

Gulati, Gaspers, Dann et al.: "Amino acids activate mTOR complex 1 via Ca2+/CaM signaling to hVps34." in: Cell metabolism, Vol. 7, Issue 5, pp. 456-65, 2008 (PubMed).

Pattingre, Bauvy, Carpentier et al.: "Role of JNK1-dependent Bcl-2 phosphorylation in ceramide-induced macroautophagy." in: The Journal of biological chemistry, Vol. 284, Issue 5, pp. 2719-28, 2009 (PubMed).