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Details for Product No. ABIN268927

Beclin 1, Autophagy Related (BECN1) antibody

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Antigen
Synonyms ATG6, VPS30, beclin1, 4921513J16Rik, 5430417M23Rik, Atg6, zgc:73097, beclin-1, atg6, vps30, Beclin-1, becn1
Reactivity
»Alternatives Human, Mouse (Murine)
Host
»Alternatives Rabbit
Clonality Polyclonal
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
Pubmed 9 references available
Catalog no. ABIN268927
Quantity 25 µL
Price
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Immunogen ABIN153048 was raised against an internal synthetic peptide to human Beclin 1,containing residues within 1-100. This sequence has 94% identity with the mousesequence and 88% identity with the rat sequence.
Specificity This antibody is specific to human Beclin 1. Species Reactivity: This antibody can be used for detection of Beclin 1 in human samples. Preliminaryresults suggest that this antibody may cross-react with mouse protein, as well. Otherspecies have not been tested. Localization: Cell Membrane, Cytoplasmic and Golgi Apparatus. Expressed in dendrites and cellbodies of cerebellar Purkinje cells.
Cross-Reactivity Human, Mouse (Murine)
Alternative Name Beclin-1
Background Beclin 1 is the first identified mammalian gene to mediate autophagy and also has tumorsuppressor and antiviral function. Autophagy, a process of bulk protein degradationthrough an autophagosomic-lysosomal pathway, is important for differentiation, survivalduring nutrient deprivation, and normal growth control, and is often defective in tumorcells. Beclin 1 was originally isolated in a yeast two hybrid screen to identifyBcl-2-binding partners and maps to a tumor susceptibility locus on human chromosome17q21 that is frequently monoallelically deleted in human breast, ovarian and prostatecancer. Beclin 1 encodes an evolutionarily conserved 60 kDa coiled coil protein that isexpressed in human muscle, epithelial cells and neurons. In gene transfer studies,beclin 1 promotes nutrient deprivation-induced autophagy, inhibits mammarytumorigenesis, and inhibits viral replication. Expression of the Beclin 1 protein isfrequently decreased in malignant breast epithelial cells. Based upon theseobservations, it is speculated that beclin 1 may work through induction of autophagy tonegatively regulate tumorigenesis and to control viral infections. Beclin 1 may also playa role in other biologic processes in which autophagy is important such as celldifferentiation and nutritional stress responses. Alternate Names: anti-CTIP-1 COUP-TF interacting protein 1 antibody, anti-Beclin1 autophagy antibody,anti-Coiled-coil myosin-like BCL2-interacting protein antibody, anti-Protein GT197antibody, anti-APG6. GENE NAME: BECN1 antibody.
Gene ID 8678
Application Notes Suggested working dilutions: Western Blot 1:2000-1:5000,Immunohistochemistry-Paraffin
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/ml
Buffer Tris-citrate/phosphate, pH 7-8. Preservative: 0.1% sodium azide.
Preservative Sodium azide
Storage 4 °C
Liang, Kleeman, Jiang et al.: "Protection against fatal Sindbis virus encephalitis by beclin, a novel Bcl-2-interacting protein." in: Journal of virology, Vol. 72, Issue 11, pp. 8586-96, 1998 (PubMed).

Aita, Liang, Murty et al.: "Cloning and genomic organization of beclin 1, a candidate tumor suppressor gene on chromosome 17q21." in: Genomics, Vol. 59, Issue 1, pp. 59-65, 1999 (PubMed).

Liang, Jackson, Seaman et al.: "Induction of autophagy and inhibition of tumorigenesis by beclin 1." in: Nature, Vol. 402, Issue 6762, pp. 672-6, 2000 (PubMed).

Massey, Kaushik, Sovak et al.: "Consequences of the selective blockage of chaperone-mediated autophagy." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 15, pp. 5805-10, 2006 (PubMed).

Park, Lee, Kim et al.: "A human scFv antibody against TRAIL receptor 2 induces autophagic cell death in both TRAIL-sensitive and TRAIL-resistant cancer cells." in: Cancer research, Vol. 67, Issue 15, pp. 7327-34, 2007 (PubMed).

Wang, Singh, Massey et al.: "Loss of macroautophagy promotes or prevents fibroblast apoptosis depending on the death stimulus." in: The Journal of biological chemistry, Vol. 283, Issue 8, pp. 4766-77, 2008 (PubMed).

Massey, Follenzi, Kiffin et al.: "Early cellular changes after blockage of chaperone-mediated autophagy." in: Autophagy, Vol. 4, Issue 4, pp. 442-56, 2008 (PubMed).

Gulati, Gaspers, Dann et al.: "Amino acids activate mTOR complex 1 via Ca2+/CaM signaling to hVps34." in: Cell metabolism, Vol. 7, Issue 5, pp. 456-65, 2008 (PubMed).

Pattingre, Bauvy, Carpentier et al.: "Role of JNK1-dependent Bcl-2 phosphorylation in ceramide-induced macroautophagy." in: The Journal of biological chemistry, Vol. 284, Issue 5, pp. 2719-28, 2009 (PubMed).

Alternatives for antigen "Beclin 1, Autophagy Related (BECN1)", type "Antibodies"
Hosts (134), (62), (3)
Reactivities (174), (92), (58), (47), (34), (23), (20), (15), (12), (8), (2), (1), (1)
Applications (186), (75), (51), (44), (43), (23), (19), (19), (10), (8), (4), (2), (1)
Conjugates (10), (10), (8), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (22), (13), (7), (4), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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