Beclin 1 antibody (Beclin 1, Autophagy Related) (AA 1-100)

Details for Product anti-BECN1 Antibody No. ABIN268927, Supplier: Log in to see
Antigen
  • beclin-1
  • atg6
  • vps30
  • beclin1
  • Beclin-1
  • becn1
  • zgc:73097
  • ATG6
  • VPS30
  • 4921513J16Rik
  • 5430417M23Rik
  • Atg6
  • beclin 1, autophagy related
  • beclin 1
  • beclin 1 protein
  • beclin-1
  • Beclin-1
  • beclin 1 (coiled-coil, myosin-like BCL2 interacting protein)
  • beclin-1-like
  • BECN1
  • becn1
  • atg6
  • CpipJ_CPIJ011087
  • PTRG_07825
  • SJAG_01876
  • Becn1
  • LOC100352353
Alternatives
anti-Human Beclin 1 antibody for Immunohistochemistry (Paraffin-embedded Sections)
Epitope
AA 1-100
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26
20
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12
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3
3
3
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2
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2
2
1
1
1
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Reactivity
Human, Mouse (Murine), Rat (Rattus)
430
154
145
34
23
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18
14
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13
8
6
3
3
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2
1
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Host
Rabbit
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117
3
Clonality
Polyclonal
Conjugate
This Beclin 1 antibody is un-conjugated
23
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23
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19
8
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7
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5
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2
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2
1
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1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Simple Western (SimWes), Western Blotting (WB)
344
178
161
112
106
72
61
26
26
23
13
6
3
2
1
1
Options
Supplier
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Supplier Product No.
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Immunogen Internal synthetic peptide to human Beclin 1, within residues 1-100 [UniProt# Q14457].
Purification Immunogen affinity purified
Alternative Name Beclin 1 (BECN1 Antibody Abstract)
Background Gene Symbol: BECN1
Gene ID 8678
UniProt Q14457
Research Area Transcription Factors, Autophagy, Cancer
Pathways Autophagy
Application Notes Western Blot 1:500-1:2000, Simple Western 1:50, Immunohistochemistry 1:400, Immunocytochemistry/Immunofluorescence 1:50-1:200, Immunoprecipitation 1:80, Immunohistochemistry-Paraffin 1:400This Beclin 1 Antibody is useful for Western Blot, Immunoprecipitation, Immunocytochemistry and Immunohistochemistry paraffin-embedded sections. In Western Blot, a band is seen at ~52 kDa representing Beclin 1. In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Immunoprecipitation protocol specific for Beclin 1 Antibody Immunoprecipitation Protocol:
1. Cells in 2x 75cm flasks (60 % confluency) are scraped with 0.5ml of Tris lysis
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS ( pH 7.4)
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-Beclin 1, Autophagy Related (BECN1) (AA 1-100) antibody (ABIN268927) Detection of Beclin in mouse liver tissue lysate (50 ug) using ABIN153048. ECL detec...
Immunohistochemistry (IHC) image for anti-Beclin 1, Autophagy Related (BECN1) (AA 1-100) antibody (ABIN268927) Pheochromocytes of the Adrenal Medulla 40x
Immunohistochemistry (IHC) image for anti-Beclin 1, Autophagy Related (BECN1) (AA 1-100) antibody (ABIN268927) Immunohistochemistry: Beclin 1/ATG6 Antibody [ABIN153048] - ATG6 on pheochromocytes o...
Simple Western (SimWes) image for anti-Beclin 1, Autophagy Related (BECN1) (AA 1-100) antibody (ABIN268927) Simple Western: Beclin 1/ATG6 Antibody [ABIN153048] - Simple Western lane view shows ...
Western Blotting (WB) image for anti-Beclin 1, Autophagy Related (BECN1) (AA 1-100) antibody (ABIN268927) Western Blot: Beclin 1/ATG6 Antibody [ABIN153048] - WB analysis of Belclin1. Lane 1 h...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Beclin 1, Autophagy Related (BECN1) (AA 1-100) antibody (ABIN268927) Immunohistochemistry-Paraffin: Beclin 1/ATG6 Antibody [ABIN153048] - Analysis of Becl...
Immunofluorescence (IF) image for anti-Beclin 1, Autophagy Related (BECN1) (AA 1-100) antibody (ABIN268927) Immunocytochemistry/Immunofluorescence: Beclin 1/ATG6 Antibody [ABIN153048] - Beclin ...
Product cited in: Eritja, Chen, Rodríguez-Barrueco, Santacana, Gatius, Vidal, Martí, Ponce, Bergadà, Yeramian, Encinas, Ribera, Reventós, Boyd, Villanueva, Matias-Guiu, Dolcet, Llobet-Navàs: "Autophagy orchestrates adaptive responses to targeted therapy in endometrial cancer." in: Autophagy, Vol. 13, Issue 3, pp. 608-624, 2017 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Human).

Ugun-Klusek, Tatham, Elkharaz, Constantin-Teodosiu, Lawler, Mohamed, Paine, Anderson, John Mayer, Lowe, Ellen Billett, Bedford: "Continued 26S proteasome dysfunction in mouse brain cortical neurons impairs autophagy and the Keap1-Nrf2 oxidative defence pathway." in: Cell death & disease, Vol. 8, Issue 1, pp. e2531, 2017 (PubMed). Method employed by authors: Immunohistochemistry (IHC) (Sample species: Mouse (Murine)).

Huang, Ao, Li, Wu, Xu, Deng, Chen, Yin, Cheng: "Autophagy Protects Advanced Glycation End Product-Induced Apoptosis and Expression of MMP-3 and MMP-13 in Rat Chondrocytes." in: BioMed research international, Vol. 2017, pp. 6341919, 2017 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Rat (Rattus)).

Gao, Zhuang, Gao, Li, Zhang, Ye, Li, Tang, Zhou, Han, Li: "Tetrahydrocurcumin reduces oxidative stress-induced apoptosis via the mitochondrial apoptotic pathway by modulating autophagy in rats after traumatic brain injury." in: American journal of translational research, Vol. 9, Issue 3, pp. 887-899, 2017 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Rat (Rattus)).

Wang, Hong, Syu, Tsai, Liu, Chen, Su, Kuo, Lin, Teng: "LRWD1 Regulates Microtubule Nucleation and Proper Cell Cycle Progression in the Human Testicular Embryonic Carcinoma Cells." in: Journal of cellular biochemistry, 2017 (PubMed).

Zhang, Ding, Wang, Wu, Xu: "Traumatic Brain Injury-Induced Neuronal Apoptosis is Reduced Through Modulation of PI3K and Autophagy Pathways in Mouse by FTY720." in: Cellular and molecular neurobiology, Vol. 36, Issue 1, pp. 131-42, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Masuda, Yashiro, Kitayama, Miki, Kasashima, Kinoshita, Morisaki, Fukuoka, Hasegawa, Sakurai, Toyokawa, Kubo, Tanaka, Muguruma, Masaichi, Hirakawa: "Clinicopathological Correlations of Autophagy-related Proteins LC3, Beclin 1 and p62 in Gastric Cancer." in: Anticancer research, Vol. 36, Issue 1, pp. 129-36, 2016 (PubMed). (Sample species: Human). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Ueno, Saji, Sugimoto, Masuda, Kuroi, Sato, Takei, Yamamoto, Ohno, Yamashita, Hisamatsu, Aogi, Iwata, Imoto, Sasano, Toi: "Clinical significance of the expression of autophagy-associated marker, beclin 1, in breast cancer patients who received neoadjuvant endocrine therapy." in: BMC cancer, Vol. 16, pp. 230, 2016 (PubMed). (Sample species: Human). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Choudhury, Bucha, Baksi, Mukhopadhyay, Bhattacharyya: "Chaperone-like protein HYPK and its interacting partners augment autophagy." in: European journal of cell biology, Vol. 95, Issue 6-7, pp. 182-94, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Kivinen, Felszeghy, Kinnunen, Setälä, Aikio, Kinnunen, Sironen, Pihlajaniemi, Kauppinen, Kaarniranta: "Absence of collagen XVIII in mice causes age-related insufficiency in retinal pigment epithelium proteostasis." in: Biogerontology, Vol. 17, Issue 4, pp. 749-61, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Hong, Kim, Lee: "Role of necroptosis in autophagy signaling during hepatic ischemia and reperfusion." in: Toxicology and applied pharmacology, Vol. 308, pp. 1-10, 2016 (PubMed). Further details: Western Blotting

Cheong, Kim, Eom, Jeung, Min: "Enhanced autophagy in cytarabine arabinoside-resistant U937 leukemia cells and its potential as a target for overcoming resistance." in: Molecular medicine reports, Vol. 13, Issue 4, pp. 3433-40, 2016 (PubMed). Method employed by authors: Western Blotting (WB)

Wang, Pei, Chen, Zhang: "Increased Apoptosis of Inflamed Odontoblasts Is Associated with CD47 Loss." in: Journal of dental research, Vol. 95, Issue 6, pp. 697-703, 2016 (PubMed).

Jang, Eom, Jeung, Cheong, Lee, Kim, Min: "AMPK-ULK1-mediated autophagy confers resistance to BET inhibitor JQ1 in acute myeloid leukemia stem cells." in: Clinical cancer research : an official journal of the American Association for Cancer Research, 2016 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Human).

Hu, Zou, Huang, Zhang, Lourenco, Chen, Halliday, Wang, Ren: "ROCK1 Is Associated with Alzheimer's Disease-Specific Plaques, as well as Enhances Autophagosome Formation But not Autophagic Aβ Clearance." in: Frontiers in cellular neuroscience, Vol. 10, pp. 253, 2016 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).

Shen, Chen, He, Chen, Song, Xiao, Wan, Dai, Pan, Wang: "Inhibition of ERα/ERK/P62 cascades induces "autophagic switch" in the estrogen receptor-positive breast cancer cells exposed to gemcitabine." in: Oncotarget, Vol. 7, Issue 30, pp. 48501-48516, 2016 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Human).

Bortnik, Choutka, Horlings, Leung, Baker, Lebovitz, Dragowska, Go, Bally, Minchinton, Gelmon, Gorski: "Identification of breast cancer cell subtypes sensitive to ATG4B inhibition." in: Oncotarget, Vol. 7, Issue 41, pp. 66970-66988, 2016 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Human).

Yang, Guo, Liao, Cao, Liang, He: "BAMBI inhibits inflammation through the activation of autophagy in experimental spinal cord injury." in: International journal of molecular medicine, Vol. 39, Issue 2, pp. 423-429, 2016 (PubMed). (Sample species: Rat (Rattus)).

Wakabayashi, Mori, Kakita, Takahashi, Utsumi, Sasaki: "Analysis of microRNA from archived formalin-fixed paraffin-embedded specimens of amyotrophic lateral sclerosis." in: Acta neuropathologica communications, Vol. 2, pp. 173, 2015 (PubMed). (Sample species: Human). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Casalino-Matsuda, Nair, Beitel, Gates, Sporn: "Hypercapnia Inhibits Autophagy and Bacterial Killing in Human Macrophages by Increasing Expression of Bcl-2 and Bcl-xL." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 194, Issue 11, pp. 5388-96, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting

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