Reacts with CD55, decay-accelerating factor (DAF), a glycosylphosphatidylinositol (GPI)-anchored single chain glycoprotein of approximately 70 kDa, expressed on hematopoietic cells. It has been suggested that the role of DAF is to protect cells from damage by autologous complement preventing the amplification steps of the complement cascade by interfering with the assembly of the C3-convertases, C4b2a and C3bBb, and the C5-convertases, C4b2a3b and C3bBb3b. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Profile of K562 cells expressing glycosylphosphatidylinositol (GPI) anchored protein analyzed on a FACScan Profile of glycosylphosphatidylinositol (GPI) achor-defective mutant K562 cell line lymphocytes analyzed on a FACScan
Optimal working dilution should be determined by the investigator.
Sample Volume
20 μL
Restrictions
For Research Use only
Buffer
Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
Storage
4 °C
Storage Comment
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Loveland, Szokolai, Johnstone, McKenzie: "Coordinate functions of multiple complement regulating molecules, CD46, CD55, and CD59." in: Transplantation proceedings, Vol. 26, Issue 3, pp. 1070-1, (1994) (PubMed).
Kinoshita, Medof, Silber, Nussenzweig: "Distribution of decay-accelerating factor in the peripheral blood of normal individuals and patients with paroxysmal nocturnal hemoglobinuria." in: The Journal of experimental medicine, Vol. 162, Issue 1, pp. 75-92, (1985) (PubMed).
Guesdon, Ternynck, Avrameas: "The use of avidin-biotin interaction in immunoenzymatic techniques." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 27, Issue 8, pp. 1131-9, (1979) (PubMed).