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CD55 antibody (Biotin)

CD55 Reactivity: Human FACS Host: Mouse Monoclonal IA10 Biotin
Catalog No. ABIN2689338
  • Target See all CD55 Antibodies
    CD55 (Complement Decay-Accelerating Factor (CD55))
    Reactivity
    • 109
    • 35
    • 7
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    Human
    Host
    • 68
    • 67
    • 6
    • 6
    • 3
    • 1
    • 1
    Mouse
    Clonality
    • 81
    • 69
    • 2
    Monoclonal
    Conjugate
    • 72
    • 17
    • 16
    • 11
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This CD55 antibody is conjugated to Biotin
    Application
    • 97
    • 77
    • 47
    • 39
    • 32
    • 25
    • 16
    • 15
    • 14
    • 13
    • 9
    • 4
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS)
    Brand
    BD Pharmingen™
    Characteristics
    Reacts with CD55, decay-accelerating factor (DAF), a glycosylphosphatidylinositol (GPI)-anchored single chain glycoprotein of approximately 70 kDa, expressed on hematopoietic cells. It has been suggested that the role of DAF is to protect cells from damage by autologous complement preventing the amplification steps of the complement cascade by interfering with the assembly of the C3-convertases, C4b2a and C3bBb, and the C5-convertases, C4b2a3b and C3bBb3b. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Profile of K562 cells expressing glycosylphosphatidylinositol (GPI) anchored protein analyzed on a FACScan Profile of glycosylphosphatidylinositol (GPI) achor-defective mutant K562 cell line lymphocytes analyzed on a FACScan

    BD Pharmingen™ Biotin Mouse Anti-Human CD55 - Biotin - Clone IA10 - Isotype Mouse IgG2a, κ - Reactivity Hu - 100 Tests
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Clone
    IA10
    Isotype
    IgG2a kappa
    Top Product
    Discover our top product CD55 Primary Antibody
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Sample Volume
    20 μL
    Restrictions
    For Research Use only
  • Buffer
    Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
    Storage
    4 °C
    Storage Comment
    Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
  • Loveland, Szokolai, Johnstone, McKenzie: "Coordinate functions of multiple complement regulating molecules, CD46, CD55, and CD59." in: Transplantation proceedings, Vol. 26, Issue 3, pp. 1070-1, (1994) (PubMed).

    Kinoshita, Medof, Silber, Nussenzweig: "Distribution of decay-accelerating factor in the peripheral blood of normal individuals and patients with paroxysmal nocturnal hemoglobinuria." in: The Journal of experimental medicine, Vol. 162, Issue 1, pp. 75-92, (1985) (PubMed).

    Guesdon, Ternynck, Avrameas: "The use of avidin-biotin interaction in immunoenzymatic techniques." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 27, Issue 8, pp. 1131-9, (1979) (PubMed).

  • Target
    CD55 (Complement Decay-Accelerating Factor (CD55))
    Alternative Name
    CD55 (CD55 Products)
    Synonyms
    CR antibody, CROM antibody, DAF antibody, TC antibody, Daf antibody, Daf-GPI antibody, Daf1 antibody, GPI-DAF antibody, CD55 molecule (Cromer blood group) antibody, CD55 molecule, decay accelerating factor for complement antibody, CD55 antibody, Cd55 antibody
    Background
    Synonyms: DAF
    Pathways
    Complement System, Regulation of Leukocyte Mediated Immunity
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