CD59 antibody (Biotin)

Catalog No. ABIN2689347

Product Details anti-CD59 Antibody (Biotin)

Target: CD59

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD59 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Brand: BD Pharmingen™

Characteristics: Reacts with CD59, a 19 kDa glycosylphosphatidylinositol (GPI)-anchored glycoprotein, expressed on hematopoietic and non-hematopoietic cells. Because of its role in interaction with complement activated products, CD59 has been termed membrane-attack-complex-inhibitory factor (MACIF), homologus restriction factor (HRF20), membrane inhibitor of reactive lysis (MIRL) and protectin. It inhibits the cytolytic activity of complement by binding to C8 and C9, thereby blocking the assembly of the membrane attack complex. CD59 also participates in spontaneous T-cell/erythrocyte adhesion, interacts with CD2 and plays a role in T-cell activation. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Profile of glycosylphosphatidylinositol (GPI) anchor-defective mutant cell line analyzed on a FACScan Profile of K562 cells expressing glycosylphosphatidylinositol (GPI) anchor protein analyzed on a FACScan

BD Pharmingen™ Biotin Mouse Anti-Human CD59 - Biotin - Clone p282 (H19) - Isotype Mouse IgG2a, κ - Reactivity Hu - 100 Tests

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Clone: P282

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Sample Volume: 20 μL

Restrictions: For Research Use only

Handling

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-CD59 antibody (Biotin) (ABIN2689347)

Davies, Lachmann: "Membrane defence against complement lysis: the structure and biological properties of CD59." in: Immunologic research, Vol. 12, Issue 3, pp. 258-75, (1994) (PubMed).

Deckert, Kubar, Bernard: "CD58 and CD59 molecules exhibit potentializing effects in T cell adhesion and activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 148, Issue 3, pp. 672-7, (1992) (PubMed).

Deckert, Kubar, Zoccola, Bernard-Pomier, Angelisova, Horejsi, Bernard: "CD59 molecule: a second ligand for CD2 in T cell adhesion." in: European journal of immunology, Vol. 22, Issue 11, pp. 2943-7, (1992) (PubMed).

Guesdon, Ternynck, Avrameas: "The use of avidin-biotin interaction in immunoenzymatic techniques." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 27, Issue 8, pp. 1131-9, (1979) (PubMed).

Target Details for CD59

Target: CD59

Alternative Name: CD59 (CD59 Products)

Synonyms: 16.3A5 antibody, 1F5 antibody, EJ16 antibody, EJ30 antibody, EL32 antibody, G344 antibody, HRF-20 antibody, HRF20 antibody, MAC-IP antibody, MACIF antibody, MEM43 antibody, MIC11 antibody, MIN1 antibody, MIN2 antibody, MIN3 antibody, MIRL antibody, MSK21 antibody, p18-20 antibody, Cd59a antibody, Cd59b antibody, MACIP antibody, CD59 molecule (CD59 blood group) antibody, CD59 molecule antibody, CD59 antibody, Cd59 antibody

Pathways: Complement System