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HLA Class I Histocompatibility Antigen, alpha Chain E (HLAE) antibody

Details for Product No. ABIN268938, Supplier: Log in to see
Antigen
  • EA1.2
  • EA2.1
  • HLA-6.2
  • MHC
  • QA1
Reactivity
Human
101
4
4
4
2
2
1
Host
Mouse
60
41
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
6
6
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
50
40
31
19
19
15
11
3
3
2
1
1
Supplier
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Immunogen Recombinant human HLA-E denatured heavy chain.
Clone MEM-E-02
Isotype IgG1
Specificity This reacts with the denaturated heavy chain of human HLA-E.
Purification Affinity purified
Alternative Name HLA class I alpha E / HLA-E (HLAE Antibody Abstract)
Background HLA E belongs to the HLA class I heavy chain paralogues. This class I molecule is aheterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). Theheavy chain is anchored in the membrane. HLA E binds a restricted subset of peptidesderived from the leader peptides of other class I molecules. Alternate Names: anti-HLA class I histocompatibility antigen E alpha chain precursor antibody,anti-HLA-6.2 antibody, anti-Major histocompatibility complex class I E antibody.
Gene Symbol: HLA-E
Gene ID 3133
Pathways
Application Notes IHC-P: Use at an assay dependant dilution. WB: Use at an assay dependant dilution. Predicted molecular weight: 43 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.
Recommended dilutions: Western Blot 1:1000, Immunohistochemistry-Paraffin 1:200, Immunohistochemistry 1:10-1:500
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer PBS with [pH 7.4], Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C
Storage Comment 4 °C short term. Aliquot and store at -20 °C long term.
Product cited in: Mavilio, Lombardo, Kinter et al.: "Characterization of the defective interaction between a subset of natural killer cells and dendritic cells in HIV-1 infection." in: The Journal of experimental medicine, Vol. 203, Issue 10, pp. 2339-50, 2006 (PubMed).

Menier, Saez, Horejsi et al.: "Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: new tools to analyze the expression of nonclassical HLA class I molecules." in: Human immunology, Vol. 64, Issue 3, pp. 315-26, 2003 (PubMed).