CD8 antibody

Catalog No. ABIN2689403

Product Details anti-CD8 Antibody

Target: CD8

Reactivity: Human, Rhesus Monkey, Cynomolgus, Baboon

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD8 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Brand: BD Pharmingen™

Characteristics: The RPA-T8 monoclonal antibody specifically binds to CD8 alpha (CD8α). CD8α is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily. CD8α is expressed by the majority of thymocytes, by subpopulations of αβ T cells and γδ T cells and by some NK cells. Cell surface CD8α is expressed either as a disulfide-linked homodimer (CD8αα) or as a heterodimer (CD8αβ) when disulfide-bonded to a CD8 beta chain (CD8β). CD8-positive αβ T cells coexpress both CD8αα homodimers and CD8αβ heterodimers whereas some γδ T cells and NK cells express CD8αα homodimers. CD8 plays important roles in T cell activation and selection. The extracellular IgSF domain of CD8α binds to a non-polymorphic determinant on HLA class I molecules (α3 domain) and enables CD8 to function as a co-receptor with MHC class I-restricted TCR during T cell recognition of antigen. The cytoplasmic domain of CD8α associates with Lck, a Src family protein tyrosine kinase that is involved in intracellular signaling. The RPA-T8 and HIT8a monoclonal antibodies are not cross-blocking. This clone has been reported to react with a subset of peripheral blood lymphocytes, but not monocytes nor granuloyctes, of baboon and both rhesus and cynomolgus macaque monkey. In general, a higher frequency of CD8+ and CD4+CD8+ lymphocytes are observed in non-human primates compared to normal human donors. Profile of anti-CD8 reactivity on peripheral blood lymphocytes of rhesus macaque (macaca mulatta) analyzed by flow cytometry.

BD Pharmingen™ Purified Mouse Anti-Human CD8 - Purified - Clone RPA-T8 - Isotype Mouse IgG1, κ - Reactivity Hu, Bab, Cyno, Rhe - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Clone: RPA

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-CD8 antibody (ABIN2689403)

Sopper, Stahl-Hennig, Demuth, Johnston, Dörries, ter Meulen: "Lymphocyte subsets and expression of differentiation markers in blood and lymphoid organs of rhesus monkeys." in: Cytometry, Vol. 29, Issue 4, pp. 351-62, (1998) (PubMed).

Target Details for CD8

Target: CD8

Alternative Name: CD8 (CD8 Products)

Synonyms: CD8 antibody, Leu2 antibody, MAL antibody, p32 antibody, CD8a molecule antibody, CD8A antibody

Background: Synonyms: CD8α, CD8A, CD8 alpha, Leu2, MAL, T8, p32