CD80 antibody

Catalog No. ABIN2689405

Product Details anti-CD80 Antibody

Target: CD80

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD80 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Brand: BD Pharmingen™

Characteristics: Reacts with a 60 kD transmembrane glycoprotein which was clustered as CD80 in the Fifth International Workshop on Human Differentiation Antigens. There have been several recent studies reporting discrepancies in the expression and function of CD80 when the BB1 clone is used compared to other anti-CD80 monoclonals (e.g., L307.4). Caution should therefore be used in the interpretation of data generated using BB1, as reports indicate that BB1 may recognize, in addition to CD80, CD74 and even possibly another as yet undefined molecule. CD80, a member of the Ig supergene family, is expressed on activated B cells, macrophages, and dendritic cells. It is the ligand for two molecules expressed on T cells: CD28 and CD152 (CTLA-4). CD80 is also expressed in activated CD4+ and CD8+ T cells, appearing late after activation, suggesting that activated T cells may be capable of autocrine costimulation via the CD28 activation pathway. The binding of CD28 Molecule by CD28 mAb or by CD80 antigen results in T-cell activation and a signal for IL-2 production. Profile of RAJI cells analyzed by flow cytometry

BD Pharmingen™ Purified Mouse Anti-Human CD80 - Purified - Clone BB1 - Isotype Mouse IgM, κ - Reactivity Hu - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Clone: BB1

Isotype: IgM kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-CD80 antibody (ABIN2689405)

Azuma, Yssel, Phillips, Spits, Lanier: "Functional expression of B7/BB1 on activated T lymphocytes." in: The Journal of experimental medicine, Vol. 177, Issue 3, pp. 845-50, (1993) (PubMed).

Schwartz: "Costimulation of T lymphocytes: the role of CD28, CTLA-4, and B7/BB1 in interleukin-2 production and immunotherapy." in: Cell, Vol. 71, Issue 7, pp. 1065-8, (1993) (PubMed).

Koulova, Clark, Shu, Dupont: "The CD28 ligand B7/BB1 provides costimulatory signal for alloactivation of CD4+ T cells." in: The Journal of experimental medicine, Vol. 173, Issue 3, pp. 759-62, (1991) (PubMed).

Target Details for CD80

Target: CD80

Alternative Name: CD80 (CD80 Products)

Synonyms: B7 antibody, B7-1 antibody, B7.1 antibody, BB1 antibody, CD28LG antibody, CD28LG1 antibody, LAB7 antibody, B71 antibody, Cd28l antibody, Ly-53 antibody, Ly53 antibody, MIC17 antibody, TSA1 antibody, CD80 molecule antibody, Cd80 molecule antibody, CD80 antigen antibody, CD80 antibody, Cd80 antibody

Background: Synonyms: B7-1

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints