CD86 antibody

Catalog No. ABIN2689420

Product Details anti-CD86 Antibody

Target: CD86

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD86 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Blocking Reagent (BR)

Brand: BD Pharmingen™

Characteristics: The GL1 antibody has been reported to react with the B7-2 (CD86) costimulatory molecule expressed on a broad spectrum of leukocytes, including B lymphocytes, T lymphocytes, thioglycollate-induced peritoneal macrophages, dendritic cells and astrocytes. CD86 is expressed at low levels by freshly explanted peripheral B and T cells, and its expression is substantially increased by a variety of T cell- and B cell-specific stimuli with a peak expression after 18-42 hours of culture. In contrast to most naive CD4+ T cells, memory CD4+ T cells express B7-2, both at the mRNA and protein level. CD86, a ligand for CD28 and CD152 (CTLA-4), is one of the accessory molecules that plays an important role in T cell-B cell costimulatory interactions. It has been shown to be involved in immunoglobulin class-switching and triggering of mouse NK cell-mediated cytotoxicity. CD80 (B7-1) is an alternate ligand for CD28 and CD152 (CTLA-4). GL1 antibody reportedly blocks MLR and stimulation of T cells by natural antigen-presenting cells. In addition, a mixture of anti-B7-1 and anti B7-2 (GL1) mAbs reportedly inhibits the in vitro interaction of CTLA-4 with its ligand and the in vivo priming of cytotoxic T lymphocytes. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Upregulation of membrane CD86 (B7-2) on activated B lymphocytes. Freshly isolated (left panel) or 72-hour LPS-stimulated BALB/c splenocytes (right panel) were pretreated with Mouse BD Fc Block™ purified anti-mouse CD16/CD32 mAb 2.4G2 (Cat. No. 553141/553142) and either unstained (shaded histograms) or stained with purified GL1 mAb (open histograms) followed by FITC-conjugated anti-rat IgG2a mAb RG7/1.30 (Cat. No. 553896, shaded and open histograms). Flow cytometry was performed on a BD FACScan™

BD Pharmingen™ Purified Rat Anti-Mouse CD86 - Purified - Clone GL1 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse (CBA/Ca) LPS-activated splenic B Cells

Clone: GL1

Isotype: IgG2a kappa

Application Details

Application Notes: Flow Cytometry System. Resting lymphocytes (left panel) or activated blasts (right panel) were selected according to light-scatter profile.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-CD86 antibody (ABIN2689420)

Turley, Inaba, Garrett, Ebersold, Unternaehrer, Steinman, Mellman: "Transport of peptide-MHC class II complexes in developing dendritic cells." in: Science (New York, N.Y.), Vol. 288, Issue 5465, pp. 522-7, (2000) (PubMed).

Martín-Fontecha, Assarsson, Carbone, Kärre, Ljunggren: "Triggering of murine NK cells by CD40 and CD86 (B7-2)." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 10, pp. 5910-6, (1999) (PubMed).

McAdam, Schweitzer, Sharpe: "The role of B7 co-stimulation in activation and differentiation of CD4+ and CD8+ T cells." in: Immunological reviews, Vol. 165, pp. 231-47, (1999) (PubMed).

Hakamada-Taguchi, Kato, Ushijima, Murakami, Uede, Nariuchi: "Expression and co-stimulatory function of B7-2 on murine CD4+ T cells." in: European journal of immunology, Vol. 28, Issue 3, pp. 865-73, (1998) (PubMed).

Borriello, Sethna, Boyd, Schweitzer, Tivol, Jacoby, Strom, Simpson, Freeman, Sharpe: "B7-1 and B7-2 have overlapping, critical roles in immunoglobulin class switching and germinal center formation." in: Immunity, Vol. 6, Issue 3, pp. 303-13, (1997) (PubMed).

Herold, Vezys, Koons, Lenschow, Thompson, Bluestone: "CD28/B7 costimulation regulates autoimmune diabetes induced with multiple low doses of streptozotocin." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 2, pp. 984-91, (1997) (PubMed).

Liu, Wenger, Zhao, Nielsen: "Distinct costimulatory molecules are required for the induction of effector and memory cytotoxic T lymphocytes." in: The Journal of experimental medicine, Vol. 185, Issue 2, pp. 251-62, (1997) (PubMed).

Nikcevich, Gordon, Tan, Hurst, Kroepfl, Gardinier, Barrett, Miller: "IFN-gamma-activated primary murine astrocytes express B7 costimulatory molecules and prime naive antigen-specific T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 2, pp. 614-21, (1997) (PubMed).

Bluestone: "New perspectives of CD28-B7-mediated T cell costimulation." in: Immunity, Vol. 2, Issue 6, pp. 555-9, (1995) (PubMed).

Hathcock, Laszlo, Pucillo, Linsley, Hodes: "Comparative analysis of B7-1 and B7-2 costimulatory ligands: expression and function." in: The Journal of experimental medicine, Vol. 180, Issue 2, pp. 631-40, (1994) (PubMed).

Inaba, Witmer-Pack, Inaba, Hathcock, Sakuta, Azuma, Yagita, Okumura, Linsley, Ikehara, Muramatsu, Hodes, Steinman: "The tissue distribution of the B7-2 costimulator in mice: abundant expression on dendritic cells in situ and during maturation in vitro." in: The Journal of experimental medicine, Vol. 180, Issue 5, pp. 1849-60, (1994) (PubMed).

Larsen, Ritchie, Hendrix, Linsley, Hathcock, Hodes, Lowry, Pearson: "Regulation of immunostimulatory function and costimulatory molecule (B7-1 and B7-2) expression on murine dendritic cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 11, pp. 5208-19, (1994) (PubMed).

Lenschow, Su, Zuckerman, Nabavi, Jellis, Gray, Miller, Bluestone: "Expression and functional significance of an additional ligand for CTLA-4." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 23, pp. 11054-8, (1994) (PubMed).

Freeman, Borriello, Hodes, Reiser, Hathcock, Laszlo, McKnight, Kim, Du, Lombard: "Uncovering of functional alternative CTLA-4 counter-receptor in B7-deficient mice." in: Science (New York, N.Y.), Vol. 262, Issue 5135, pp. 907-9, (1993) (PubMed).

Hathcock, Laszlo, Dickler, Bradshaw, Linsley, Hodes: "Identification of an alternative CTLA-4 ligand costimulatory for T cell activation." in: Science (New York, N.Y.), Vol. 262, Issue 5135, pp. 905-7, (1993) (PubMed).

Target Details for CD86

Target: CD86

Alternative Name: CD86 (CD86 Products)

Synonyms: B7-2 antibody, B7.2 antibody, B70 antibody, CD28LG2 antibody, LAB72 antibody, B7 antibody, CLS1 antibody, Cd28l2 antibody, ETC-1 antibody, Ly-58 antibody, Ly58 antibody, MB7 antibody, MB7-2 antibody, TS/A-2 antibody, CD86 molecule antibody, CD86 antigen antibody, integrin subunit alpha 2b antibody, CD86 antibody, Cd86 antibody, Itga2b antibody

Background: Synonyms: B7-2

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation