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FAS antibody

FAS Reactivity: Human, Pig, Rhesus Monkey, Dog, Baboon, Cynomolgus FACS, IHC (fro) Host: Mouse Monoclonal DX2 unconjugated
Catalog No. ABIN2689460
  • Target See all FAS Antibodies
    FAS (TNF Receptor Superfamily, Member 6 (FAS))
    Reactivity
    • 214
    • 67
    • 62
    • 19
    • 6
    • 4
    • 2
    • 2
    • 1
    • 1
    Human, Pig, Rhesus Monkey, Dog, Baboon, Cynomolgus
    Host
    • 159
    • 89
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    Mouse
    Clonality
    • 156
    • 100
    • 1
    Monoclonal
    Conjugate
    • 124
    • 27
    • 25
    • 12
    • 10
    • 7
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This FAS antibody is un-conjugated
    Application
    • 165
    • 117
    • 74
    • 65
    • 36
    • 35
    • 31
    • 26
    • 26
    • 20
    • 19
    • 9
    • 8
    • 6
    • 5
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Brand
    BD Pharmingen™
    Characteristics
    Reacts with the 45 kDa transmembrane cell surface Fas or Apo-1 antigen (designated CD95 at the Fifth HLDA Workshop) which is expressed on a variety of normal and neoplastic cells. The Fas/Apo-1 antigen is a polypeptide, which plays a role in the programmed sequence of events leading to cell death, termed apoptosis. The DX2 clone specifically reacts with murine L cells, murine L1210 leukemia cells and murine P815 mastocytoma cells transfected with human Fas cDNA but not with untransfected parental cell lines. Cross-linking with DX2 delivers an apoptotic signal indicating that DX2 recognizes a functional epitope of the CD95 antigen. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Profile of peripheral blood lymphocytes analyzed by flow cytometry DX2-induced apoptosis in Fas transfectant cells line P825/FASDT151 detected by DNA fragmentation analysis. Cells were incubated alone (lane 1), in the presence of mouse IgG1 (lane 2) or in the presence of DX2 at 10, 100 or 500 ng/mL (lanes 3, 4 and 5, respectively).

    BD Pharmingen™ Purified Mouse Anti-Human CD95 - Purified - Clone DX2 - Isotype Mouse IgG1, κ - Reactivity Hu, Bab, Cyno, Dog, Pig, Rhe - 0.1 mg
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Clone
    DX2
    Isotype
    IgG1 kappa
    Top Product
    Discover our top product FAS Primary Antibody
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Restrictions
    For Research Use only
  • Concentration
    0.5 mg/mL
    Buffer
    Aqueous buffered solution containing ≤0.09 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Store undiluted at 4°C.
  • Cifone, De Maria, Roncaioli, Rippo, Azuma, Lanier, Santoni, Testi: "Apoptotic signaling through CD95 (Fas/Apo-1) activates an acidic sphingomyelinase." in: The Journal of experimental medicine, Vol. 180, Issue 4, pp. 1547-52, (1994) (PubMed).

    Itoh, Yonehara, Ishii, Yonehara, Mizushima, Sameshima, Hase, Seto, Nagata: "The polypeptide encoded by the cDNA for human cell surface antigen Fas can mediate apoptosis." in: Cell, Vol. 66, Issue 2, pp. 233-43, (1991) (PubMed).

  • Target
    FAS (TNF Receptor Superfamily, Member 6 (FAS))
    Alternative Name
    CD95 (FAS Products)
    Synonyms
    ALPS1A antibody, APO-1 antibody, APT1 antibody, CD95 antibody, FAS1 antibody, FASTM antibody, TNFRSF6 antibody, AI196731 antibody, APO1 antibody, TNFR6 antibody, Tnfrsf6 antibody, lpr antibody, Fas cell surface death receptor antibody, Fas (TNF receptor superfamily member 6) antibody, FAS antibody, Fas antibody, fas antibody
    Background
    Synonyms: Fas/APO-1
    Pathways
    p53 Signaling, Apoptosis, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity
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