CD99 antibody

Catalog No. ABIN2689467

Product Details anti-CD99 Antibody

Target: CD99

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD99 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Brand: BD Pharmingen™

Characteristics: Recognizes CD99, also referred to as E2 antigen, a 32 kDa sialoglycoprotein expressed on all leukocyte lineages. The E2 antigen is the MIC2 gene product and is differentially expressed during T- and B-lymphoid and granulocytic development, with higher densities being expressed during early hematopoietic stages. Mature granulocytes express very little or no CD99. E2 has been shown to be involved in T-cell adhesion processes and is suggested to have a functional role in hematopoietic adhesion pathways. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Profile of peripheral blood lymphocytes analyzed by flow cytometry. Second step staining with Cat. No. 555988.

BD Pharmingen™ Purified Mouse Anti-Human CD99 - Purified - Clone Tü12 - Isotype Mouse IgG2a, κ - Reactivity Hu - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Clone: Tü12

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-CD99 antibody (ABIN2689467)

Dworzak, Fritsch, Buchinger, Fleischer, Printz, Zellner, Schöllhammer, Steiner, Ambros, Gadner: "Flow cytometric assessment of human MIC2 expression in bone marrow, thymus, and peripheral blood." in: Blood, Vol. 83, Issue 2, pp. 415-25, (1994) (PubMed).

Aussel, Bernard, Breittmayer, Pelassy, Zoccola, Bernard: "Monoclonal antibodies directed against the E2 protein (MIC2 gene product) induce exposure of phosphatidylserine at the thymocyte cell surface." in: Biochemistry, Vol. 32, Issue 38, pp. 10096-101, (1993) (PubMed).

Gelin, Aubrit, Phalipon, Raynal, Cole, Kaczorek, Bernard: "The E2 antigen, a 32 kd glycoprotein involved in T-cell adhesion processes, is the MIC2 gene product." in: The EMBO journal, Vol. 8, Issue 11, pp. 3253-9, (1989) (PubMed).

Target Details for CD99

Target: CD99

Alternative Name: CD99 (CD99 Products)

Synonyms: 1110061M03Rik antibody, 2410026K10Rik antibody, D4 antibody, Pilr-l antibody, pilr-1 antibody, HBA71 antibody, MIC2 antibody, MIC2X antibody, MIC2Y antibody, MSK5X antibody, CD99 antigen antibody, CD99 molecule (Xg blood group) antibody, CD99 molecule antibody, Cd99 antibody, CD99 antibody

Target Type: Viral Protein