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Vimentin antibody (VIM)

Details for Product anti-VIM Antibody No. ABIN268950, Supplier: Log in to see
Antigen
  • vime
  • CTRCT30
  • cb28
  • vim
  • vim1
  • vim2
  • VIM
  • Vimentin
  • vimentin
  • Vimentin
  • vimentin-like
  • VIM
  • vim
  • vime
  • Vim
  • vim-a
  • LOC100522394
Alternatives
anti-Human Vimentin antibody for Immunohistochemistry (Frozen Sections)
Reactivity
Human, Mammalian, Mouse (Murine), Rat (Rattus)
814
292
253
204
121
111
95
77
75
42
38
32
30
29
25
7
6
4
3
2
2
1
1
1
1
1
1
Host
Mouse
523
281
30
15
12
8
7
Clonality (Clone)
Monoclonal ()
Conjugate
This Vimentin antibody is un-conjugated
27
26
23
18
14
13
12
11
11
11
11
11
11
11
11
9
5
5
5
3
2
2
2
2
2
2
2
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
651
295
269
268
245
221
216
109
79
23
15
11
10
8
8
5
3
3
3
3
1
1
1
1
Options
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Immunogen Pellet of pig brain cold stable proteins after depolymerization of microtubules (NM_003380.3).
Clone VI-01
Isotype IgM
Specificity The antibody VI-01 reacts with vimentin, a 57 kDa intermediate filament expressed in variety of mesenchymal and mesodermal cell types. Cross-reactivity was found with smooth muscle desmin.
Purification Ammonium sulfate precipitation
Alternative Name Vimentin (VIM Antibody Abstract)
Background Gene Symbol: VIM
Gene ID 7431
UniProt P08670
Research Area Lineage Markers, Cytoskeleton, Neural Stem Cell marker, Cell/Tissue Markers
Pathways TCR Signaling, Caspase Cascade in Apoptosis
Application Notes Western Blot 1:1000, Immunocytochemistry/Immunofluorescence 1:10-1:2000Immunocytochemistry - Staining technique: RBL rat basophilic leukemia cell line:(a) Fix cells for 10 min in methanol at -20C and for 6 min in acetone at -20C, (b) Fix cells directly in methanol for 10 min at -20oC or in acetone for 10 min at -20oC. Incubation: 45 min RT
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer TBS (approx. pH 8.0)
Buffer contains: 15 mM Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.
Supplier Images
Immunofluorescence (IF) image for anti-Vimentin antibody (VIM) (ABIN268950) Immunocytochemistry/Immunofluorescence: Vimentin Antibody (VI-01) [ABIN268950] - Stai...
Immunofluorescence (IF) image for anti-Vimentin antibody (VIM) (ABIN268950) Immunocytochemistry/Immunofluorescence: Vimentin Antibody (VI-01) [ABIN268950] - Stai...
Western Blotting (WB) image for anti-Vimentin antibody (VIM) (ABIN268950) Western Blot: Vimentin Antibody (VI-01) [ABIN268950] - Analysis of Vimentin in whole ...
Western Blotting (WB) image for anti-Vimentin antibody (VIM) (ABIN268950) Western Blot: Vimentin Antibody (VI-01) [ABIN268950] - HeLa cell lysate by antibody V...
Product cited in: Rankin, Tomaszewski, Haase: "Renal cyst development in mice with conditional inactivation of the von Hippel-Lindau tumor suppressor." in: Cancer research, Vol. 66, Issue 5, pp. 2576-83, 2006 (PubMed).

Deramaudt, Takaoka, Upadhyay, Bowser, Porter, Lee, Rhoades, Johnstone, Weissleder, Hingorani, Mahmood, Rustgi et al.: "N-cadherin and keratinocyte growth factor receptor mediate the functional interplay between Ki-RASG12V and p53V143A in promoting pancreatic cell migration, invasion, and tissue architecture ..." in: Molecular and cellular biology, Vol. 26, Issue 11, pp. 4185-200, 2006 (PubMed).

Background publications Lukás, Dráber, Bucek, Dráberová, Viklický, Dolezel: "Expression of phosphorylated high molecular weight neurofilament protein (NF-H) and vimentin in human developing dorsal root ganglia and spinal cord." in: Histochemistry, Vol. 100, Issue 6, pp. 495-502, 1994 (PubMed).

Lukás, Dráber, Bucek, Dráberová, Viklický, Stasková: "Expression of vimentin and glial fibrillary acidic protein in human developing spinal cord." in: The Histochemical journal, Vol. 21, Issue 12, pp. 693-701, 1990 (PubMed).

Dráberová, Dráber, Havlícek, Viklický: "A common antigenic determinant of vimentin and desmin defined by monoclonal antibody." in: Folia biologica, Vol. 32, Issue 5, pp. 295-303, 1989 (PubMed).