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Vimentin (VIM) antibody

Details for Product No. ABIN268950
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Antigen
Synonyms vime, CTRCT30, cb28, vim, vim1, vim2, VIM, Vimentin
Reactivity
Pig (Porcine)
(469), (162), (151), (85), (78), (53), (41), (18), (12), (11), (10), (9), (6), (5), (5), (2), (2), (1), (1), (1), (1), (1), (1)
Host
Mouse
(253), (225), (26), (9), (7), (5), (4)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(20), (19), (12), (6), (5), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (2), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
(390), (167), (164), (143), (101), (96), (61), (49), (40), (34), (3), (3), (3), (2), (2), (1), (1), (1)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN268950
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Immunogen Pellet of pig brain cold stable proteins after depolymerization of microtubules (NM_003380.3).
Clone VI-01
Isotype IgM
Specificity Besides reactivity with vimentin, this also cross-reacts with smooth muscle desmin. A positive reaction is also found on thyroid folicular cells and several mesothelial cells.
Purification Affinity purified
Alternative Name Vimentin
Background Vimentin is the major subunit protein of the intermediate filaments of mesenchymalcells. It is believed to be involved with the intracellular transport of proteins between thenucleus and plasma membrane. Vimentin has been implicated to be involved in the rateof steroid synthesis via its role as a storage network for steroidogenic cholesterolcontaining lipid droplets. Vimentin phosphorylation by a protein kinase causes thebreakdown of intermediate filaments and activation of an ATP and myosin light chaindependent contractile event. This results in cytoskeletal changes that facilitate theinteraction of the lipid droplets within mitochondria, and subsequent transport ofcholesterol to the organelles leading to an increase in steroid synthesis.Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural,muscle and fibroblast origin) compared to carcinomas which are generally negative.Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentinantibodies are thus of value in the differential diagnosis of undifferentiated neoplasmsand malignant tumors. They are generally used with a panel of other antibodiesincluding those recognizing cytokeratins, lymphoid markers, S100, desmin andneurofilaments. Alternate Names: anti-FLJ36605 antibody, anti-VIM antibody.
Gene Symbol: VIM
Gene ID 7431
NCBI Accession NM_003380.3
Research Area Lineage Markers, Cytoskeleton, Neural Stem Cell marker, Cell/Tissue Markers
Application Notes ELISA: Use at an assay dependant concentration. ICC: Use at an assay dependant concentration. WB: Use at an assay dependant concentration. Unsuitable for IP. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.
Recommended dilutions: Western Blot 1:1000, Immunocytochemistry/Immunofluorescence 1:10-1:2000
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer PBS [pH 7.4], Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
General Dráberová, Dráber, Havlícek et al.: "A common antigenic determinant of vimentin and desmin defined by monoclonal antibody." in: Folia biologica, Vol. 32, Issue 5, pp. 295-303, 1989 (PubMed).

Lukás, Dráber, Bucek et al.: "Expression of vimentin and glial fibrillary acidic protein in human developing spinal cord." in: The Histochemical journal, Vol. 21, Issue 12, pp. 693-701, 1990 (PubMed).

Lukás, Dráber, Bucek et al.: "Expression of phosphorylated high molecular weight neurofilament protein (NF-H) and vimentin in human developing dorsal root ganglia and spinal cord." in: Histochemistry, Vol. 100, Issue 6, pp. 495-502, 1994 (PubMed).

Rankin, Tomaszewski, Haase: "Renal cyst development in mice with conditional inactivation of the von Hippel-Lindau tumor suppressor." in: Cancer research, Vol. 66, Issue 5, pp. 2576-83, 2006 (PubMed).

Deramaudt, Takaoka, Upadhyay et al.: "N-cadherin and keratinocyte growth factor receptor mediate the functional interplay between Ki-RASG12V and p53V143A in promoting pancreatic cell migration, invasion, and tissue architecture disruption." in: Molecular and cellular biology, Vol. 26, Issue 11, pp. 4185-200, 2006 (PubMed).

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