Vimentin (VIM) antibody

Antigen: Vimentin (VIM)

Synonyms: FLJ36605, MGC102095, cb28, MGC139781, VIM, vim, Vim, vime

Clonality: Monoclonal (VI-01)

Host: Mouse

Reactivity: Please enquire

Conjugate: Un-conjugated

Application: Immunofluorescence (IF), Western Blotting (WB), ELISA

Catalog no.: ABIN268950

Additional Information

Alternative name: Vimentin

Immunogen: Pellet of pig brain cold stable proteins after depolymerization of microtubules.

Isotype: IgM

Clone: VI-01

Description: Vimentin is the major subunit protein of the intermediate filaments of mesenchymalcells. It is believed to be involved with the intracellular transport of proteins between thenucleus and plasma membrane. Vimentin has been implicated to be involved in the rateof steroid synthesis via its role as a storage network for steroidogenic cholesterolcontaining lipid droplets. Vimentin phosphorylation by a protein kinase causes thebreakdown of intermediate filaments and activation of an ATP and myosin light chaindependent contractile event. This results in cytoskeletal changes that facilitate theinteraction of the lipid droplets within mitochondria, and subsequent transport ofcholesterol to the organelles leading to an increase in steroid synthesis.Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural,muscle and fibroblast origin) compared to carcinomas which are generally negative.Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentinantibodies are thus of value in the differential diagnosis of undifferentiated neoplasmsand malignant tumors. They are generally used with a panel of other antibodiesincluding those recognizing cytokeratins, lymphoid markers, S100, desmin andneurofilaments. Alternate Names: anti-FLJ36605 antibody, anti-VIM antibody.

Specificity: Besides reactivity with vimentin, this antibody also cross-reacts with smooth muscledesmin. A positive reaction is also found on thyroid folicular cells and severalmesothelial cells. Species Reactivity: Cross-reacts with all species (recognized epitope conserved within all species). Localization: Cytoplasmic.

Application Details

Buffer: In PBS with 15mM Sodium Azide. pH 7.4

Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Research Area: Lineage Markers, Cytoskeleton, Neural Stem Cell marker, Cell/Tissue Markers

Restrictions: For Research Use only

Publications

Druaberovua, Druaber, Havluicek et al.: "A common antigenic determinant of vimentin and desmin defined by monoclonal antibody." in: Folia biologica, Vol. 32, Issue 5, pp. 295-303, 1989 (PubMed).

Lukuas, Druaber, Bucek et al.: "Expression of vimentin and glial fibrillary acidic protein in human developing spinal cord." in: The Histochemical journal, Vol. 21, Issue 12, pp. 693-701, 1990 (PubMed).

Lukuas, Druaber, Bucek et al.: "Expression of phosphorylated high molecular weight neurofilament protein (NF-H) and vimentin in human developing dorsal root ganglia and spinal cord." in: Histochemistry, Vol. 100, Issue 6, pp. 495-502, 1994 (PubMed).

Rankin, Tomaszewski, Haase: "Renal cyst development in mice with conditional inactivation of the von Hippel-Lindau tumor suppressor." in: Cancer research, Vol. 66, Issue 5, pp. 2576-83, 2006 (PubMed).

Deramaudt, Takaoka, Upadhyay et al.: "N-cadherin and keratinocyte growth factor receptor mediate the functional interplay between Ki-RASG12V and p53V143A in promoting pancreatic cell migration, invasion, and tissue architecture disruption." in: Molecular and cellular biology, Vol. 26, Issue 11, pp. 4185-200, 2006 (PubMed).