EMR4 antibody

Catalog No. ABIN2689502

Product Details anti-EMR4 Antibody

Target: EMR4 (EGF-Like Module Containing, Mucin-Like, Hormone Receptor-Like Sequence 4 (EMR4))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This EMR4 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)

Brand: BD Pharmingen™

Characteristics: The 6F12 antibody reacts with a 7-transmembrane-domain protein, which is similar to the F4/80 macrophage antigen of the EGF-TM7 protein family and is encoded by the Emr4 gene. The FIRE protein is expressed on myeloid cells with a denditic cell (DC) developmental potential, including subsets of DC and macrophages in the spleen and lymph nodes, most resident peritoneal macrophages, many peripheral blood monocytes, and a subpopulation of bone-marrow myeloid-cell progenitors. The protein is not detected on peripheral T and B lymphocytes, and it is down-regulated on thioglycollate-elicited peritoneal macrophages and on dendritic cells activated by GM-CSF, IFN-γ, anti-CD40, and LPS. Using soluble biotinylated fusion protein, a FIRE ligand was detected on a mouse IgG+ B lymphoma cell line (A20), but not on myeloid, fibroblast, or T-cell lines, suggesting that the FIRE protein may be involved in immunoregulatory interactions between antigen-presenting cells and B lymphocytes. Three-analysis of the expression of FIRE on bone-marrow leukocytes. BALB/c bone-marrow leukocytes were stained with either purified rat IgG2a, κ isotype control mAb R35-95 (Cat. No. 553297, left panels) or purified mAb 6F12 (right panels), in the presence of Mouse BD Fc Block™ purified anti-mouse CD16/CD32 mAb 2.4G2 (Cat. No. 553141/553142), followed by biotinylated anti-rat IgG2a mAb RG7/1.30 (Cat. No. 553894), then Streptavidin-APC (Cat. No. 554067). Myeloid cells were identified by staining with FITC-conjugated anti-mouse CD11b mAb M1/70 (Cat. No. 557396/553310, upper panels)and dendritic cells were identified with PE-conjugated anti-mouse Cd11c mAb HL3 (Cat. No. 557401/553802, bottom panels). The right panels demonstrate that the hematopoietic precursors which express FIRE protein are CD11b[intermediate]CD11c-. Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.

BD Pharmingen™ Purified Rat Anti-Mouse F4/80-Like Receptor - Purified - Clone 6F12 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: CHO cells expressing recombinant FIRE fusion protein

Clone: 6F12

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

Target Details for EMR4

Target: EMR4 (EGF-Like Module Containing, Mucin-Like, Hormone Receptor-Like Sequence 4 (EMR4))

Alternative Name: F4/80-Like Receptor (EMR4 Products)

Synonyms: D17Ertd479e antibody, Egf-tm7 antibody, Fire antibody, Gpr127 antibody, adhesion G protein-coupled receptor E4 antibody, Adgre4 antibody

Background: Synonyms: FIRE