GZMA antibody

Catalog No. ABIN2689529

Product Details anti-GZMA Antibody

Target: GZMA (Granzyme A (Granzyme 1, Cytotoxic T-Lymphocyte-Associated serine Esterase 3) (GZMA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GZMA antibody is un-conjugated

Application: Immunoprecipitation (IP), Flow Cytometry (FACS)

Brand: BD Pharmingen™

Characteristics: The primary mechanism by which cytotoxic T cells eliminate virally infected cells is by granule exocytosis. The release of cytotoxic granule contents by cytotoxic T lymphocytes (CTL) triggers apoptotic target cell death. CTL granules contain a pore-forming protein, perforin, and a group of serine proteases called granzymes. In the classic model, perforins create holes in the target cell membrane, allowing entrance of the granzymes. Granzyme A and B are the predominant granzymes activated after CTL activation, but each act via an independent apoptotic pathway, granzyme B is activated immediately, while granzyme A acts hours later. Granzyme B has been shown to induce apoptosis and to cleave a number of substrates which are similar in specificity to those of the caspase family of proteinases. Granzyme B can cleave substrates, such as DNA-PKcs, and nuclear mitotic apparatus protein (NuMA). Furthermore, Granzyme B can also cleave substrates such as Bid and DFF45 in a caspase-independent fashion. Studies involving mice which are deficient in both granzyme A and B suggest a model whereby the granzyme B pathway may have evolved as the major apoptotic pathway with the granzyme A pathway acting as a backup. However, further research is needed to delineate the components of these distinct pathways.

BD Pharmingen™ Purified Mouse Anti-Human Granzyme A - Purified - Clone CB9 - Isotype Mouse IgG1, κ - Reactivity Hu - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Clone: CB9

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-GZMA antibody (ABIN2689529)

Barry, Heibein, Pinkoski, Lee, Moyer, Green, Bleackley: "Granzyme B short-circuits the need for caspase 8 activity during granule-mediated cytotoxic T-lymphocyte killing by directly cleaving Bid." in: Molecular and cellular biology, Vol. 20, Issue 11, pp. 3781-94, (2000) (PubMed).

Beresford, Xia, Greenberg, Lieberman: "Granzyme A loading induces rapid cytolysis and a novel form of DNA damage independently of caspase activation." in: Immunity, Vol. 10, Issue 5, pp. 585-94, (1999) (PubMed).

Shresta, Graubert, Thomas, Raptis, Ley: "Granzyme A initiates an alternative pathway for granule-mediated apoptosis." in: Immunity, Vol. 10, Issue 5, pp. 595-605, (1999) (PubMed).

Andrade, Roy, Nicholson, Thornberry, Rosen, Casciola-Rosen: "Granzyme B directly and efficiently cleaves several downstream caspase substrates: implications for CTL-induced apoptosis." in: Immunity, Vol. 8, Issue 4, pp. 451-60, (1998) (PubMed).

Trimble, Lieberman: "Circulating CD8 T lymphocytes in human immunodeficiency virus-infected individuals have impaired function and downmodulate CD3 zeta, the signaling chain of the T-cell receptor complex." in: Blood, Vol. 91, Issue 2, pp. 585-94, (1998) (PubMed).

Beresford, Kam, Powers, Lieberman: "Recombinant human granzyme A binds to two putative HLA-associated proteins and cleaves one of them." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 17, pp. 9285-90, (1997) (PubMed).

Target Details for GZMA

Target: GZMA (Granzyme A (Granzyme 1, Cytotoxic T-Lymphocyte-Associated serine Esterase 3) (GZMA))

Alternative Name: Granzyme A (GZMA Products)

Synonyms: gzmA antibody, GZMA antibody, AW494114 antibody, Ctla-3 antibody, Ctla3 antibody, Hf antibody, SE1 antibody, TSP-1 antibody, TSP1 antibody, CTLA3 antibody, HFSP antibody, granzyme A antibody, Granzyme A antibody, granzyme A (granzyme 1, cytotoxic T-lymphocyte-associated serine esterase 3) antibody, gzmA antibody, GZMA antibody, graa antibody, Gzma antibody

Pathways: Apoptosis