Interleukin 17a antibody

Catalog No. ABIN2689741

Product Details anti-Interleukin 17a Antibody

Target: Interleukin 17a (IL17A) (Interleukin 17A (IL17A))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This Interleukin 17a antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Neutralization (Neut), Blocking Antibody (Inhibition)

Brand: BD Pharmingen™

Characteristics: The TC11-18H10 antibody reacts with recombinant and natural mouse IL-17A proteins. IL-17A, also known as CTLA-8, is a T cell-derived cytokine that promotes inflammatory responses. Mouse IL-17A is a proinflammatory cytokine that can induce the release of IL-6 by mouse stromal cells. It has been shown to support the growth of hemopoietic progenitors in vitro, it can also stimulate granulopoiesis in vivo. The TC11-18H10 antibody has been reported to neutralize IL-17A activity. Recent studies have shown that IL-17A is produced by a unique subset of Th17 cells that develop along a pathway distinct from the Th1- and Th2- cell differentiation pathways. The mouse IL-17A cDNA was isolated from a cDNA library generated from TCRαβ+CD4-CD8-thymocytes. Characterization of IL-17A-producing cells within a stimulated mouse EL4 thymoma cell population. EL4 cells were cultured overnight in the presence of GolgiPlug™ (1 μg/mL final concentration, Cat. No. 555029) without (left panel) or with PMA (5 ng/mL final concentration, Sigma, Cat. No. P-8139) and Ionomycin (500 ng/mL final concentration, Sigma, Cat. No.I-0634) (middle and right panels). The cells were fixed, permeabilized and subsequently stained with 0.125 μg of PE conjugated anti-mouse IL-17A (PE-TC11-18H10, Cat. No.559502) using BD Pharmingen I/C staining protocol (left and middle panels) . To demonstrate the specificity of staining, the binding of PE-TC11-18H10 was blocked by the preincubation of the fixed/permeabilized, stimulated EL4 cells with unlabeled TC11-18H10 antibody (10 μg, right panel). The quadrant markers for the bivariate dot plots were set based on the autofluorescence control, and verified with the unstimulated stained (left panel) and unlabeled antibody blocking (right panel) controls.

BD Pharmingen™ Purified Rat Anti-Mouse IL-17A - Purified - Clone TC11-18H10 - Isotype Rat IgG1, κ - Reactivity Ms - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Recombinant Mouse IL-17 Protein

Clone: TC11

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-Interleukin 17a antibody (ABIN2689741)

Schwarzenberger, La Russa, Miller, Ye, Huang, Zieske, Nelson, Bagby, Stoltz, Mynatt, Spriggs, Kolls: "IL-17 stimulates granulopoiesis in mice: use of an alternate, novel gene therapy-derived method for in vivo evaluation of cytokines." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 11, pp. 6383-9, (1998) (PubMed).

Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, (1996) (PubMed).

Target Details for Interleukin 17a

Target: Interleukin 17a (IL17A) (Interleukin 17A (IL17A))

Alternative Name: IL-17A (IL17A Products)

Synonyms: CTLA8 antibody, IL-17 antibody, IL-17A antibody, IL17 antibody, Ctla-8 antibody, Ctla8 antibody, Il17 antibody, ChIL-17 antibody, IL-17F antibody, IL17A antibody, CTLA-8 antibody, interleukin 17A antibody, IL17A antibody, Il17a antibody