The MM17F8F5 monoclonal antibody specifically binds to Interleukin-17F (IL-17F) and can neutralize its biological activity. IL-17F is a member of the IL-17 family of cytokines. Among IL-17 family members, IL-17F has the highest amino acid sequence homology to IL-17A. IL-17F is produced by activated CD4+ T helper (Th17) cells, CD8+ T (Tc17) cells and γδ T cells. IL-17F can be secreted as homodimers or as heterodimers with IL-17A. IL-17F and IL-17A have overlapping functions such as inducing epithelial cells and fibroblasts to produce proinflammatory cytokines and chemokines including IL-6, GM-CSF, CXCL1, CCL2, and CCL7. These factors attract and activate neutrophils and other cell types that mediate protective responses against pathogenic microbes or pathologic allergic or autoimmune diseases. IL-17 gene knockout studies have shown that IL-17F and IL-17A have independent functions as well. IL-17F and IL-17A exert their biological function by binding to and signaling through IL-17 receptors comprised of the transmembrane receptor subunits, IL-17RA (CD217) and IL-17RC. Neutralization of mouse IL-17F biological activity with MM17F8F5, a Mouse Anti-Mouse IL-17F monoclonal antibody. In a 96-well microplate, biologically-active recombinant mouse IL-17F protein (0.2 μg/well) was preincubated (1 hr, 37 °C) with serial dilutions of Purified NA/LE Mouse Anti-Mouse IL-17F antibody (Cat. No. 563592, μg/mL) or tissue culture medium (as a control). Following the incubation, cells from the NIH-3T3 mouse embryonic cell line were added at 5 x 10^4 cells per well and were cultured at 37 °C. After 24 hours of culture, the supernatants from each well were harvested and their concentrations of IL-6 were quantified using a BD™ Cytometric Bead Array Mouse IL-6 Flex Set (Cat. No. 558301). As the MM17F8F5 antibody was serially diluted, a neutralizing Anti-Mouse IL-17F antibody dose-response relationship was observed as shown in the figure. 563592 Rev. 1 Page 1 of 2
IL17F
Reactivity: Human
WB, ELISA, Neut
Host: Rabbit
Polyclonal
Biotin
Application Notes
Optimal working dilution should be determined by the investigator.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
Preservative
Without preservative
Handling Advice
This preparation contains no preservatives, thus it should be handled under aseptic conditions.
Storage
4 °C
Storage Comment
Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
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