Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

IL-2 antibody

IL2 Reactivity: Mouse ELISA, IP, Inhibition Host: Rat Monoclonal JES6 unconjugated
Catalog No. ABIN2689752
  • Target See all IL-2 (IL2) Antibodies
    IL-2 (IL2) (Interleukin 2 (IL2))
    Reactivity
    • 128
    • 89
    • 42
    • 14
    • 9
    • 8
    • 6
    • 6
    • 5
    • 4
    • 4
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Mouse
    Host
    • 111
    • 79
    • 59
    • 10
    • 4
    • 2
    • 1
    Rat
    Clonality
    • 138
    • 124
    • 1
    Monoclonal
    Conjugate
    • 145
    • 37
    • 23
    • 22
    • 14
    • 9
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This IL-2 antibody is un-conjugated
    Application
    • 156
    • 123
    • 69
    • 63
    • 40
    • 28
    • 24
    • 22
    • 14
    • 14
    • 13
    • 13
    • 13
    • 11
    • 11
    • 8
    • 6
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Immunoprecipitation (IP), Blocking Antibody (Inhibition)
    Brand
    BD Pharmingen™
    Characteristics
    The JES6-5H4 antibody reacts with mouse interleukin-2 (IL-2) and has been reported to be neutralizing. Expression of IL-2 by stimulated CD4+ and CD4- BALB/c spleen cells. Splenocytes from 6 month old BALB/c mice were stimulated for 5 hours with hamster anti-mouse CD3 (2 μg/mL final concentration, clone 145-2C11, Cat. No. 553057) and hamster anti-mouse CD28 (2 μg/mL final concentration, clone 37.51, Cat. No. 553294) antibodies in the presence of BD GolgiStop™ (3 μM final concentration, Cat. No. 554724). The cells were harvested, stained with 0.06 μg of PE-conjugated rat anti-mouse CD4 (PE-RM4-5, Cat. No. 553049), fixed, permeabilized, and subsequently stained with 0.06 μg of FITC-conjugated rat anti-mouse IL-2 antibody (FITC-JES6-5H4, Cat. No. 554427, left panel). To demonstrate specificity of staining, the binding by the FITC-JES6-5H4 antibody was blocked by preincubation of the conjugated antibody with recombinant mouse IL-2 (0.12 μg, Cat. No. 550069, middle panel), and by preincubation of the fixed/permeabilized cells with unlabeled JES6-5H4 antibody (2.5 μg, Cat. No. 554425, right panel) prior to staining. The quadrant markers for the bivariate dot plots were set based on the autofluorescence controls and verified using the recombinant cytokine blocking and unlabelled antibody blocking specificity controls.

    BD Pharmingen™ Purified Rat Anti-Mouse IL-2 - Purified - Clone JES6-5H4 - Isotype Rat IgG2b - Reactivity Ms - 0.1 mg
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogen
    Mouse IL-2 Recombinant Protein
    Clone
    JES6
    Isotype
    IgG2b
    Top Product
    Discover our top product IL2 Primary Antibody
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Restrictions
    For Research Use only
  • Concentration
    0.5 mg/mL
    Buffer
    Aqueous buffered solution containing ≤0.09 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Store undiluted at 4°C.
  • Abrams: "Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies." in: Current protocols in immunology / edited by John E. Coligan ... [et al.], Vol. Chapter 6, pp. Unit 6.20, (2008) (PubMed).

    Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, (1996) (PubMed).

    Abrams, Roncarolo, Yssel, Andersson, Gleich, Silver: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, (1992) (PubMed).

  • Target
    IL-2 (IL2) (Interleukin 2 (IL2))
    Alternative Name
    IL-2 (IL2 Products)
    Synonyms
    IL2 antibody, IL-2 antibody, il2 antibody, TCGF antibody, lymphokine antibody, Il-2 antibody, POIL2 antibody, interleukin 2 antibody, interleukin-15 antibody, IL2 antibody, il2 antibody, LOC100303688 antibody, Il2 antibody
    Pathways
    JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation
You are here:
Support