IL-21 antibody

Catalog No. ABIN2689754

Product Details anti-IL-21 Antibody

Target: IL-21 (IL21) (Interleukin 21 (IL21))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL-21 antibody is un-conjugated

Application: ELISA

Brand: BD Pharmingen™

Characteristics: The J148-1134 antibody reacts with interleukin-21 (IL-21), an ~ 15 kDa member of the type-I-cytokine family of proteins. IL-21 is expressed primarily by CD4+ T cells and has a broad range of immunoregulatory functions on B cells, CD8+ T cells, NK cells and dendritic cells. The effects of IL-21 include promotion of antibody production, NK cell differentiation, proliferation and differentiation of cells of monocyte and granulocyte lineages, costimulation of T cells and augmentation of antitumor activity of CD8+ T cells. It has been speculated that IL-21, through its varies positive and negative regulatory effects on cell-mediated and humoral immunity, may play a role in autoimmuninty, allergy, asthma and tumor regression. Human IL-21 sandwich ELISA curve: The curve was generated by a sandwich ELISA using the purified J148-1134 as capture antibody, doubling dilutions of recombinant human IL- 21 protein and biotin I76-539 (Cat. No. 558502) as detection antibody. Streptavidin-HRP (Cat. No. 554066) plus TMB substrate (Cat. No. 555214)were used to develop the ELISA.

BD Pharmingen™ Purified Mouse anti-Human IL-21 - Purified - Clone J148-1134 - Isotype Mouse IgG1, κ - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human IL-21

Clone: J148

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-IL-21 antibody (ABIN2689754)

Leonard, Spolski: "Interleukin-21: a modulator of lymphoid proliferation, apoptosis and differentiation." in: Nature reviews. Immunology, Vol. 5, Issue 9, pp. 688-98, (2005) (PubMed).

Sivakumar, Foster, Clegg: "Interleukin-21 is a T-helper cytokine that regulates humoral immunity and cell-mediated anti-tumour responses." in: Immunology, Vol. 112, Issue 2, pp. 177-82, (2004) (PubMed).

Target Details for IL-21

Target: IL-21 (IL21) (Interleukin 21 (IL21))

Alternative Name: IL-21 (IL21 Products)

Synonyms: IL-21 antibody, Za11 antibody, interleukin 21 antibody, IL21 antibody, Il21 antibody

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response