IL5 antibody (Interleukin 5)

Details for Product anti-IL5 Antibody No. ABIN2689772, Supplier: Log in to see
Antigen
  • IL5
  • Il-5
  • IL-5
  • TRF
  • EDF
  • Pro-TRH
  • interleukin 5
  • Interleukin 5
  • interleukin 5 (colony-stimulating factor, eosinophil)
  • interleukin-5-like
  • thyrotropin-releasing hormone
  • IL5
  • Il5
  • IL-5
  • LOC100358075
  • TRH
Alternatives
anti-Human IL5 antibody for Enzyme Immunoassay
Reactivity
Human, Mouse (Murine)
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Host
Rat
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Clonality (Clone)
Monoclonal ()
Conjugate
This IL5 antibody is un-conjugated
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Application
Blocking Antibody (Inhibition), ELISA, Western Blotting (WB)
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Options
Supplier
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Supplier Product No.
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Brand BD Pharmingen™
Immunogen Mouse Semi-Purified T-Cell
Clone TRFK5
Isotype IgG1, kappa
Characteristics The TRFK5 antibody reacts with mouse interleukin-5 (IL-5) and cross-reacts with human IL-5. The TRFK5 antibody has been reported to cross react with IL-5 from rhesus monkey. This is a neutralizing antibody. Expression of IL-5 by stimulated mouse CD4+ T cells. Purified splenic CD4+ cells from BALB/c mice were stimulated with plate-bound anti-CD3 (25 μg/mL final concentration, clone 145-2C11, Cat. No. 553057) and soluble anti-mouse CD28 (2 μg/mL final concentration, Cat. No. 553294) for 2 days in culture together with recombinant mouse IL-2 (10 ng/mL final concentration, Cat. No. 550069) and recombinant mouse IL-4 (1 ng/mL final concentration, Cat. No. 550067), followed by a 3 day incubation with only recombinant mouse IL-2 and IL-4, This was followed by a 5 hour stimulation with plate-bound anti-CD3 (25 μg/mL final concentration) and soluble anti-mouse CD28 (2 μg/mL final concentration) in the presence of BD GolgiStop™ (2 μM final concentration, Cat. No. 554724). The cells were stained with 0.06 μg of PE Rat Anti-Mouse CD4 (Cat. No. 553049), fixed, permeabilized, and subsequently stained with 0.12 μg of FITC-conjugated Rat anti-Mouse IL-5 antibody (FITC-TRFK5) by using the BD Pharmingen™ staining protocol (left panel). To demonstrate specificity of staining, the binding of FITC-TRFK5 antibody was blocked by the preincubation of the conjugated antibody with recombinant mouse IL-5 (0.1 μg, Cat. No. 554581, see middle panel), and by preincubation of the fixed/permeabilized cells with Purified Rat Anti-Mouse/Anti-Human IL-5 antibody (5 μg, Cat. No. 554392, right panel) prior to staining with the FITC-TRFK5 antibody. The quadrant markers for the bivariate dot plots were set based on the autofluorescence control and verified with the recombinant cytokine blocking and unlabeled antibody blocking specificity controls.

BD Pharmingen™ Purified Rat Anti-Mouse/Anti-Human IL-5 - Purified - Clone TRFK5 - Isotype Rat IgG1, κ - Reactivity Ms, Hu - 0.1 mg
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name IL-5 (IL5 Antibody Abstract)
Research Area Cytokines, Immunology, Virology, Inflammation, Cancer
Pathways JAK-STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.
Product cited in: Abrams: "Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies." in: Current protocols in immunology / edited by John E. Coligan ... [et al.], Vol. Chapter 6, pp. Unit 6.20, 2008 (PubMed).

Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, 1996 (PubMed).

Mo, Sarawar, Doherty: "Induction of cytokines in mice with parainfluenza pneumonia." in: Journal of virology, Vol. 69, Issue 2, pp. 1288-91, 1995 (PubMed).

Assenmacher, Schmitz, Radbruch: "Flow cytometric determination of cytokines in activated murine T helper lymphocytes: expression of interleukin-10 in interferon-gamma and in interleukin-4-expressing cells." in: European journal of immunology, Vol. 24, Issue 5, pp. 1097-101, 1994 (PubMed).

Sarawar, Sangster, Coffman, Doherty: "Administration of anti-IFN-gamma antibody to beta 2-microglobulin-deficient mice delays influenza virus clearance but does not switch the response to a T helper cell 2 phenotype." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 3, pp. 1246-53, 1994 (PubMed).

Abrams, Roncarolo, Yssel, Andersson, Gleich, Silver: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, 1992 (PubMed).

Swain, Weinberg, English, Huston: "IL-4 directs the development of Th2-like helper effectors." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 145, Issue 11, pp. 3796-806, 1991 (PubMed).

Schumacher, OGarra, Shrader, van Kimmenade, Bond, Mosmann, Coffman: "The characterization of four monoclonal antibodies specific for mouse IL-5 and development of mouse and human IL-5 enzyme-linked immunosorbent." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 141, Issue 5, pp. 1576-81, 1988 (PubMed).

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