IL-7 antibody (Biotin)

Catalog No. ABIN2689783

Product Details anti-IL-7 Antibody (Biotin)

Target: IL-7 (IL7) (Interleukin 7 (IL7))

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This IL-7 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Brand: BD Pharmingen™

Characteristics: Biotin Rat Anti-Human IL-7 antibody (clone BVD10-11C10) reacts with human interleukin-7 (IL-7). This antibody can be useful as a detection antibody for sandwich ELISA measuring human IL-7 protein levels. Biotin Rat Anti-Human IL-7 antibody (clone BVD10-11C10) can be paired with Purified Rat Anti-Human IL-7 antibody (clone BVD10-40F6, Cat. No. 554493) as the capture antibody along with recombinant human IL-7 (Cat. No. 554608) as the standard. Investigators are advised to titrate to determine optimal usage concentrations (ELISA), with a suggested range of 2 μg/mL to 0.5 μg/mL for Biotin Rat Anti-Human IL-7 antibody (clone BVD10-11C10). To obtain linear standard curves, doubling dilutions of human IL-7 ranging from ~2,000 to 15 pg/mL are recommended for inclusion in each ELISA plate. This ELISA pairing has been reported to demonstrate limited to no cross-reactivity with the following cytokines: Mouse IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-12 p70, IL-15, GM-CSF, IFN-γ, MCP-1, TCA-3, TNF or Human IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-8, IL- 9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, G-CSF, GM-CSF, IFN-γ, lymphotactin, MCP-1, MCP-2, MIP-1α, MIP-1β, NT-3, PDGF-AA, sCD23 , SCF, TNF, LT-α, VEGF, rat IL-2, IL-4, IL-6, IL-10, GM-CSF, IFN-γ, TNF.

BD Pharmingen™ Biotin Rat Anti-Human IL-7 - Biotin - Clone BVD10-11C10 - Isotype Rat IgG2a - Reactivity Hu - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Recombinant Human IL-7

Clone: BVD10-11C10

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing protein stabilizer and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-IL-7 antibody (Biotin) (ABIN2689783)

Abrams: "Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies." in: Current protocols in immunology / edited by John E. Coligan ... [et al.], Vol. Chapter 6, pp. Unit 6.20, (2008) (PubMed).

Abrams, Roncarolo, Yssel, Andersson, Gleich, Silver: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, (1992) (PubMed).

Target Details for IL-7

Target: IL-7 (IL7) (Interleukin 7 (IL7))

Alternative Name: IL-7 (IL7 Products)

Synonyms: IL7 antibody, IL-7 antibody, A630026I06Rik antibody, Il-7 antibody, hlb368 antibody, interleukin 7 antibody, IL7 antibody, Il7 antibody

Pathways: JAK-STAT Signaling