KLRG1 antibody (Biotin)

Catalog No. ABIN2689801

Product Details anti-KLRG1 Antibody (Biotin)

Target: KLRG1 (Killer Cell Lectin-Like Receptor Subfamily G, Member 1 (KLRG1))

Reactivity: Mouse, Rat

Host: Golden Syrian Hamster

Clonality: Monoclonal

Conjugate: This KLRG1 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Brand: BD Pharmingen™

Characteristics: The 2F1 monoclonal antibody specifically binds to KLRG1 (Killer cell Lectin-like Receptor G1), which is the mouse homologue of the rat mast cell function-associated antigen (MAFA), on all mouse strains tested (eg, AKR/J, BALB/c, C3H/HeN, C3H.SW, C57BL/6, DBA/1, SJL, 129/J). Unlike rat MAFA, which is expressed on mast cells, mouse KLRG1 is expressed on a large subset of NK cells, lymphokine-activated killer (LAK) cells, adherent LAK (A-LAK) cells, subsets of activated CD8+ T lymphocytes, and small fractions of CD4+ and CD8+ T cells, but not mast cells. The expression of KLRG1 is correlated with reduced proliferative capacity of activated T lymphocytes or reduced effector functions of activated NK cells. This molecule is believed to play a common role in the regulation of leukocytes of both the innate and adaptive immune system. It has been observed that the 2F1 mAb stains the rat basophilic leukemia cell line, RBL-2H3, which is known to express MAFA. The KLRG1 protein is an inhibitory lectin-like type II transmembrane receptor containing a cytoplasmic motif similar to ITIM (Immunoreceptor Tyrosine-based Inhibitory Motif), its ligand has not been identified KLRG1 is expressed mainly as a homodimeric molecule consisting of two N-glycosylated subunits of approximately 30-38 kDa. The level of KLRG1 expression is reduced in MHC class I-deficient mice, although direct binding of KLRG1 to MHC class I antigens could not be detected. Cross-linking of KLRG1 by 2F1 mAb reduces TCR-mediated Ca++ mobilization and cytotoxic responses (but not IFN-γ production) by CD8+ T cells and inhibits IFN-γ and TNF-α production and redirected lysis by NK cells. Flow cytometric analysis of KLRG1 expression on mouse splenocytes. C57BL/6 splenocytes were preincubated with Mouse BD Fc Block™ purified anti-mouse CD16/CD32 mAb 2.4G2 (Cat. No. 553141/553142), then stained with a FITC mouse anti-mouse NK-1.1 antibody (Cat.No. 553164/561082) in conjunction with either a Biotin Hamster IgG2, κ Isotype Control (Cat. No. 550084, Left Panel) or the Botin Hamster anti-Mouse KLRG1 antibody (Cat.No. 550863, Right Panel) followed by Streptavidin-PE (Cat. No. 554061). Dot plots were derived from gated events based on forward and side light-scatter characteristics of viable splenocytes. Flow cytometry was performed using a BD™ LSR II flow cytometry system.

BD Pharmingen™ Biotin Hamster Anti-Mouse KLRG1 - Biotin - Clone 2F1 - Isotype Syrian Hamster IgG2, κ - Reactivity Ms, Rat - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: A-LAK from C57BL/6 mice

Clone: 2F1

Isotype: IgG2 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-KLRG1 antibody (Biotin) (ABIN2689801)

Robbins, Terrizzi, Sydora, Mikayama, Brossay: "Differential regulation of killer cell lectin-like receptor G1 expression on T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 170, Issue 12, pp. 5876-85, (2003) (PubMed).

McMahon, Zajac, Jamieson, Corral, Hammer, Ahmed, Raulet: "Viral and bacterial infections induce expression of multiple NK cell receptors in responding CD8(+) T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 169, Issue 3, pp. 1444-52, (2002) (PubMed).

Robbins, Nguyen, Takahashi, Mikayama, Biron, Brossay: "Cutting edge: inhibitory functions of the killer cell lectin-like receptor G1 molecule during the activation of mouse NK cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 168, Issue 6, pp. 2585-9, (2002) (PubMed).

Beyersdorf, Ding, Karp, Hanke: "Expression of inhibitory "killer cell lectin-like receptor G1" identifies unique subpopulations of effector and memory CD8 T cells." in: European journal of immunology, Vol. 31, Issue 12, pp. 3443-52, (2001) (PubMed).

Voehringer, Blaser, Brawand, Raulet, Hanke, Pircher: "Viral infections induce abundant numbers of senescent CD8 T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 9, pp. 4838-43, (2001) (PubMed).

Corral, Hanke, Vance, Cado, Raulet: "NK cell expression of the killer cell lectin-like receptor G1 (KLRG1), the mouse homolog of MAFA, is modulated by MHC class I molecules." in: European journal of immunology, Vol. 30, Issue 3, pp. 920-30, (2000) (PubMed).

Blaser, Kaufmann, Pircher: "Virus-activated CD8 T cells and lymphokine-activated NK cells express the mast cell function-associated antigen, an inhibitory C-type lectin." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 12, pp. 6451-4, (1999) (PubMed).

Target Details for KLRG1

Target: KLRG1 (Killer Cell Lectin-Like Receptor Subfamily G, Member 1 (KLRG1))

Alternative Name: KLRG1 (KLRG1 Products)

Synonyms: KLRG1 antibody, 2F1-Ag antibody, MAFA antibody, MAFA-L antibody, 2F1 antibody, CLEC15A antibody, MAFA-2F1 antibody, MAFA-LIKE antibody, killer cell lectin like receptor G1 antibody, killer cell lectin-like receptor subfamily G, member 1 antibody, KLRG1 antibody, Klrg1 antibody

Background: Synonyms: Klrg1, Killer cell lectin-like receptor subfamily G member 1, MAFA