Klra7 antibody

Catalog No. ABIN2689817

Product Details anti-Klra7 Antibody

Target: Klra7 (Killer Cell Lectin-Like Receptor, Subfamily A, Member 7 (Klra7))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Blocking Reagent (BR), Cytotoxicity Test (CyTox)

Brand: BD Pharmingen™

Characteristics: The 4D11 antibody reacts with Ly-49G2 (also known as LGL-1), an inhibitory receptor which is expressed on subsets of natural killer (NK) cells and DX5-positive T lymphocytes (NK-T cells) in all strains tested (e.g., AKR/N, BALB/c, C3H/HeJ, C57BL/6, CBA/J, DBA/2, SJL, 129) and on a population of memory CD8+ T lymphocytes in C57BL/6 mice. Cross-reaction of 4D11 antibody to Ly-49A[B6], Ly-49A[BALB], and Ly-49T[129/J] inhibitory receptors and Ly-49L[CBA/J] activating receptor has been reported. The proportion of NK-T cells expressing Ly-49A and Ly-49G2 is higher (2-5 fold) in thymus than in liver (immature and mature NK-T cells, respectively), and there is evidence that down-regulation of Ly-49 receptor expression is necessary for normal NK-T-cell development to occur. Most NK cells express a single allele of Ly-49A and/or Ly-49G2, although occasionally they may express more than one allele. The Ly-49 family of NK-cell receptors, members of the C-type lectin superfamily, are disulfide-linked type-II transmembrane protein homodimers with extracellular carbohydrate-recognition domains, which bind to MHC class I alloantigens. The Ly-49 family members are expressed independently, such that an individual NK or T cell may display more than one class of Ly-49 receptor homodimers. Binding of Ly-49G[B6]-expressing transfectants to H-2Dd+/H-2Ld+ ConA blasts has been demonstrated, and H-2D[d]-expressing target cells inhibit the lytic activity of Ly-49G2-expressing NK cells. The levels of the Ly-49 inhibitory receptors are down-regulated by their ligands in vivo, and the various levels of expression of a Ly-49 inhibitory receptor may affect the specificity of NK cells. Ly-49G2[+] NK cells are able to lyse target tumor cells expressing H-2[a] and H-2[b] MHC class I antigens in vitro, and they mediate allogeneic and hybrid resistance to H-2[b] bone marrow transplantation. The Ly-49A[BALB] and Ly-49A[B6] alloantigens bind to MHC class I antigens of the d and k haplotypes, and Ly-49A[+] IL-2-activated NK cells are unable to lyse target cells expressing H-2[d] and H-2[k]. In vitro studies suggest that the Ly-49G2 and Ly-49A receptors mediate negative regulation of NK-cell cytolytic activity via tyrosine phosphorylation of their ITIMs (Immunoreceptor Tyrosine-based Inhibitory Motifs). Ly-49T[129/J] has a unique ITIM sequence, and Ly-49T-transfected 293T (human kidney epithelial) cells do not bind soluble tetramers of any tested H-2 alloantigen (D[b], D[d], D[k], K[b], K[d], K[k], L[d]). Two-color analysis of Ly-49G2 expression on splenic NK cells. C57BL/6 splenocytes were simultaneously stained with PE-conjugated anti-mouse NK-1.1 mAb PK136 (Cat. No. 557391/553165, both panels) and purified 4D11 monoclonal antibody (right panel), followed by FITC conjugated goat anti-rat Ig (Cat. No. 554001, both panels) and purified 4D11 monoclonal antibody (right panel), followed by FITC-conjugated goat anti-rat Ig (Cat. No. 554016, both panels). Flow cytometry was performed on a BD FACScan™

BD Pharmingen™ Purified Rat Anti-Mouse Ly-49G2 - Purified - Clone 4D11 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Large granular lymphocytes (LGL) enriched from C57BL/6N mouse liver

Clone: 4D11

Isotype: IgG2a kappa

Application Details

Application Notes: Flow Cytometry System. 555314 Rev. 8 Page 1 of 2

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-Klra7 antibody (ABIN2689817)

Makrigiannis, Pau, Saleh, Winkler-Pickett, Ortaldo, Anderson: "Class I MHC-binding characteristics of the 129/J Ly49 repertoire." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 8, pp. 5034-43, (2001) (PubMed).

Sköld, Cardell: "Differential regulation of Ly49 expression on CD4+ and CD4-CD8- (double negative) NK1.1+ T cells." in: European journal of immunology, Vol. 30, Issue 9, pp. 2488-96, (2000) (PubMed).

Hanke, Takizawa, McMahon, Busch, Pamer, Miller, Altman, Liu, Cado, Lemonnier, Bjorkman, Raulet: "Direct assessment of MHC class I binding by seven Ly49 inhibitory NK cell receptors." in: Immunity, Vol. 11, Issue 1, pp. 67-77, (1999) (PubMed).

Ortaldo, Mason, Winkler-Pickett, Raziuddin, Murphy, Mason: "Ly-49 receptor expression and functional analysis in multiple mouse strains." in: Journal of leukocyte biology, Vol. 66, Issue 3, pp. 512-20, (1999) (PubMed).

Raulet, Held, Correa, Dorfman, Wu, Corral: "Specificity, tolerance and developmental regulation of natural killer cells defined by expression of class I-specific Ly49 receptors." in: Immunological reviews, Vol. 155, pp. 41-52, (1997) (PubMed).

Mason, Yagita, Ortaldo: "LGL-1: a potential triggering molecule on murine NK cells." in: Journal of leukocyte biology, Vol. 55, Issue 3, pp. 362-70, (1994) (PubMed).

Target Details for Klra7

Target: Klra7 (Killer Cell Lectin-Like Receptor, Subfamily A, Member 7 (Klra7))

Alternative Name: Ly-49G2 (Klra7 Products)

Synonyms: LGL-1 antibody, Ly-49G.1 antibody, Ly-49G.2 antibody, Ly-49G.3 antibody, Ly49G.4 antibody, Ly49g antibody, Ly49g1 antibody, Ly49g2 antibody, RGD1563110 antibody, killer cell lectin-like receptor, subfamily A, member 7 antibody, Klra7 antibody

Background: Synonyms: LGL-1