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LY6D antibody

LY6D Reactivity: Mouse FACS, CyTox, IHC (fro), IP Host: Rat Monoclonal 49 unconjugated
Catalog No. ABIN2689823
  • Target See all LY6D Antibodies
    LY6D (Lymphocyte Antigen 6 Complex, Locus D (LY6D))
    Reactivity
    • 9
    • 1
    Mouse
    Host
    • 7
    • 2
    Rat
    Clonality
    • 9
    Monoclonal
    Conjugate
    • 5
    • 2
    • 1
    • 1
    This LY6D antibody is un-conjugated
    Application
    Flow Cytometry (FACS), Cytotoxicity Test (CyTox), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
    Brand
    BD Pharmingen™
    Characteristics
    The 49-H4 antibody reacts with the ThB antigen (Ly-6D), which is a 15- kDa GPI-anchored protein of the Ly-6 multigene family. ThB has been detected on cortical thymocytes, small sIg- Thy-1- and sIg+ lymphoid cells in the bone marrow, thymic medullary epithelial cells, all epidermal layers (except the stratum corneum), and on peripheral B lymphocytes, but not on peripheral T lymphocytes. It has been noted that there is strain-to-strain variation in the expression of the ThB antigen on splenic B cells, those of strains with the Ly-6.2 haplotype (e.g., AKR, C57BL, DBA/2, SJL, SWR) stain more intensely with anti- ThB reagents than those of Ly-6.1 strains (e.g., A, BALB/c, CBA, C3H/He, NZB), and B cells of hybrids of Ly-6.1 and Ly-6.2 strains stain with intermediate intensity. The proportions of the ThB+ B cells and thymocytes do not differ significantly among strains.

    BD Pharmingen™ Purified Rat Anti-Mouse Ly-6D - Purified - Clone 49-H4 - Isotype Rat IgG2c, κ - Reactivity Ms - 0.1 mg
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogen
    BALB/c mouse plasmacytoma MOPC-104E
    Clone
    49
    Isotype
    IgG2c kappa
    Top Product
    Discover our top product LY6D Primary Antibody
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Restrictions
    For Research Use only
  • Concentration
    0.5 mg/mL
    Buffer
    Aqueous buffered solution containing ≤0.09 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Store undiluted at 4°C.
  • Gumley, McKenzie, Sandrin: "Polymorphism at the mouse Thb locus." in: Immunogenetics, Vol. 39, Issue 6, pp. 390-4, (1994) (PubMed).

    Boyd, Tucek, Godfrey, Izon, Wilson, Davidson, Bean, Ladyman, Ritter, Hugo: "The thymic microenvironment." in: Immunology today, Vol. 14, Issue 9, pp. 445-59, (1993) (PubMed).

    Gumley, McKenzie, Kozak, Sandrin: "Isolation and characterization of cDNA clones for the mouse thymocyte B cell antigen (ThB)." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 149, Issue 8, pp. 2615-8, (1992) (PubMed).

    Godfrey, Izon, Tucek, Wilson, Boyd: "The phenotypic heterogeneity of mouse thymic stromal cells." in: Immunology, Vol. 70, Issue 1, pp. 66-74, (1990) (PubMed).

    Matossian-Rogers, Rogers, Ledbetter, Herzenberg: "Molecular weight determination of two genetically linked cell surface murine antigens: ThB and Ly-6." in: Immunogenetics, Vol. 15, Issue 6, pp. 591-9, (1982) (PubMed).

    Eckhardt, Herzenberg: "Monoclonal antibodies to ThB detect close linkage of Ly-6 and a gene regulating ThB expression." in: Immunogenetics, Vol. 11, Issue 3, pp. 275-91, (1981) (PubMed).

  • Target
    LY6D (Lymphocyte Antigen 6 Complex, Locus D (LY6D))
    Alternative Name
    Ly-6D (LY6D Products)
    Synonyms
    E48 antibody, Ly-6D antibody, Ly-61 antibody, Ly61 antibody, Thb antibody, lymphocyte antigen 6 family member D antibody, lymphocyte antigen 6 complex, locus D antibody, LY6D antibody, Ly6d antibody
    Background
    Synonyms: ThB
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