NEUROD1 antibody

Catalog No. ABIN2689856

Product Details anti-NEUROD1 Antibody

Target: NEUROD1 (Neuronal Differentiation 1 (NEUROD1))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This NEUROD1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), BioImaging (BI), Intracellular Staining (ICS)

Brand: BD Pharmingen™

Characteristics: The R8-294 monoclonal antibody specifically binds to Neurogenic differentiation factor 1 (NeuroD1). NeuroD1 is a basic helix-loop-helix (bHLH) transcription factor that is also known as BHLHA3, Beta-cell E-box transactivator 2, BETA2 and NeuroD. NeuroD1 interacts with other bHLH proteins and binds the insulin E-box sequence to regulate pancreatic islet cell development. It also plays a key role in central nervous system (CNS) and sensory nervous system neuron development. Specifically, it is required for the terminal differentiation of neurons during late stages of neurogenesis in the CNS (cerebral cortex, hippocampus, and cerebellum) and the sensory nervous system (eye, inner ear, and olfactory system). During antibody development, the purified R8-294 monoclonal antibody was found to detect NeuroD1 by western blot and indirect immunofluorescent staining followed by flow cytometric analysis or imaging analysis. Western Blot analysis of NeuroD1 in human Retinoblastoma cell lysate. Lysate from Y79 cells (ATCC, HTB-18™) was probed with Purified Mouse Anti-NeuroD1 monoclonal antibody at titrations of 0.125 (lane 1), 0.06 (lane 2), and 0.03 μg/mL (lane 3). NeuroD1 is identified as a band of approximately 45 kDa. Immunoflourescent staining of NeuroD1 in mouse pancreatic tumor (insulinoma) cells. Beta-T-C6 cells (ATCC, CRL-11506™) were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655), permeabilized with 0.1 % Triton™ X-100 Buffer, and stained with Purified Mouse Anti-NeuroD1 monoclonal antibody (pseudo-colored green) at 1.2 μg/mL. The second-step reagent was Alexa Fluor® 488 goat anti-mouse Ig (Life Technologies), and counter-staining was with DAPI (pseudo-colored blue). The images were captured on a BD Pathway™ 435 Cell Analyzer and merged using BD AttoVision™ Software.

BD Pharmingen™ Purified Mouse Anti-NeuroD1 - Purified - Clone R8-294 - Isotype Mouse IgG1, κ - Reactivity Hu, Ms - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human NeuroD1 Recombinant Protein

Clone: R8

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-NEUROD1 antibody (ABIN2689856)

Cherry, Wang, Bormuth, Schwab, Olson, Cepko: "NeuroD factors regulate cell fate and neurite stratification in the developing retina." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 31, Issue 20, pp. 7365-79, (2011) (PubMed).

Pang, Yang, Vierbuchen, Ostermeier, Fuentes, Yang, Citri, Sebastiano, Marro, Südhof, Wernig: "Induction of human neuronal cells by defined transcription factors." in: Nature, Vol. 476, Issue 7359, pp. 220-3, (2011) (PubMed).

Gao, Ure, Ables, Lagace, Nave, Goebbels, Eisch, Hsieh: "Neurod1 is essential for the survival and maturation of adult-born neurons." in: Nature neuroscience, Vol. 12, Issue 9, pp. 1090-2, (2009) (PubMed).

Kuwabara, Hsieh, Muotri, Yeo, Warashina, Lie, Moore, Nakashima, Asashima, Gage: "Wnt-mediated activation of NeuroD1 and retro-elements during adult neurogenesis." in: Nature neuroscience, Vol. 12, Issue 9, pp. 1097-105, (2009) (PubMed).

Habener, Kemp, Thomas: "Minireview: transcriptional regulation in pancreatic development." in: Endocrinology, Vol. 146, Issue 3, pp. 1025-34, (2005) (PubMed).

Cho, Tsai: "The role of BETA2/NeuroD1 in the development of the nervous system." in: Molecular neurobiology, Vol. 30, Issue 1, pp. 35-47, (2004) (PubMed).

Tamimi, Steingrimsson, Copeland, Dyer-Montgomery, Lee, Hernandez, Jenkins, Tapscott: "The NEUROD gene maps to human chromosome 2q32 and mouse chromosome 2." in: Genomics, Vol. 34, Issue 3, pp. 418-21, (1996) (PubMed).

Naya, Stellrecht, Tsai: "Tissue-specific regulation of the insulin gene by a novel basic helix-loop-helix transcription factor." in: Genes & development, Vol. 9, Issue 8, pp. 1009-19, (1995) (PubMed).

Target Details for NEUROD1

Target: NEUROD1 (Neuronal Differentiation 1 (NEUROD1))

Alternative Name: NeuroD1 (NEUROD1 Products)

Synonyms: BETA2 antibody, BHF-1 antibody, MODY6 antibody, NEUROD antibody, bHLHa3 antibody, Neurod antibody, neurod antibody, XNeuroD antibody, MGC82572 antibody, NEUROD1 antibody, ND1 antibody, beta2 antibody, bhf-1 antibody, neuro-D antibody, nrd antibody, neuroD antibody, neurod1 antibody, Beta2 antibody, neuronal differentiation 1 antibody, neurogenic differentiation 1 antibody, neuronal differentiation 1 S homeolog antibody, neuronal differentiation 1 L homeolog antibody, NEUROD1 antibody, Neurod1 antibody, neurod1.S antibody, neurod1 antibody, neurod1.L antibody

Background: Synonyms: Class A basic helix-loop-helix protein 3, bHLHa3, BETA2, NeuroD

Molecular Weight: ~45 kDa

Pathways: Dopaminergic Neurogenesis, Hormone Transport, Carbohydrate Homeostasis