NKX6-1 antibody

Catalog No. ABIN2689864

Product Details anti-NKX6-1 Antibody

Target: NKX6-1 (NK6 Homeobox 1 (NKX6-1))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This NKX6-1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), BioImaging (BI), Intracellular Staining (ICS)

Brand: BD Pharmingen™

Characteristics: The R11-560 monoclonal antibody specifically binds to human and mouse homeobox protein Nkx6.1. The transcription factor Nkx6.1 plays a role in pancreatic islet beta cell development through the regulation of HNF1a and insulin genes. Nkx6.1 is considered to be a highly specific transcription factor marking pancreatic beta islet cells, where it is expressed in both developing and mature cells. In addition, Nkx6.1 functions as a transcriptional repressor induced by the Sonic Hedgehog (Shh) signaling pathway in fate determination of several neuronal cell types. During antibody development, the purified R11-560 monoclonal antibody was found to detect Nkx6.1 by western blot and indirect immunofluorescent staining followed by flow cytometric analysis or imaging analysis. Western Blot analysis of Nkx6.1 in mouse pancreatic tumor (insulinoma) cell lysate. Lysate from Beta-T-C6 cells (ATCC, CRL-11506™) was probed with Purified Mouse Anti-Nkx6.1 monoclonal antibody at titrations of 0.5 (lane 1), 0.25 (lane 2), and 0.125 μg/mL (lane 3). Nkx6.1 is identified as a doublet of approximately 44 kDa. Immunoflourescent staining of Nkx6.1 in mouse pancreatic tumor (insulinoma) cells. Beta-T-C6 cells (ATCC, CRL-11506™) were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655), permeabilized with BD Phosflow™ Perm III Buffer (Cat. No. 558050, and stained with Purified Mouse Anti-Nkx6.1 monoclonal antibody (pseudo-colored green) at 1.2 μg/mL. The second-step reagent was Alexa Fluor® 488 goat anti-mouse Ig (Life Technologies), and counter-staining was with DAPI (pseudo-colored blue). The images were captured on a BD Pathway™ 435 Cell Analyzer and merged using BD AttoVision™ Software.

BD Pharmingen™ Purified Mouse Anti-Nkx6.1 - Purified - Clone R11-560 - Isotype Mouse IgG1, κ - Reactivity Ms, Hu - 100 μg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Nkx6.1 Recombinant Protein

Clone: R11

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-NKX6-1 antibody (ABIN2689864)

Donelan, Koya, Li, Yang: "Distinct regulation of hepatic nuclear factor 1alpha by NKX6.1 in pancreatic beta cells." in: The Journal of biological chemistry, Vol. 285, Issue 16, pp. 12181-9, (2010) (PubMed).

Prakash, Puelles, Freude, Trümbach, Omodei, Di Salvio, Sussel, Ericson, Sander, Simeone, Wurst: "Nkx6-1 controls the identity and fate of red nucleus and oculomotor neurons in the mouse midbrain." in: Development (Cambridge, England), Vol. 136, Issue 15, pp. 2545-55, (2009) (PubMed).

Nelson, Schaffer, Sander: "The transcription factors Nkx6.1 and Nkx6.2 possess equivalent activities in promoting beta-cell fate specification in Pdx1+ pancreatic progenitor cells." in: Development (Cambridge, England), Vol. 134, Issue 13, pp. 2491-500, (2007) (PubMed).

Pedersen, Klinck, Hecksher-Sorensen, Zahn, Madsen, Serup, Jorgensen: "Generation and characterization of monoclonal antibodies against the transcription factor Nkx6.1." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 54, Issue 5, pp. 567-74, (2006) (PubMed).

DAmour, Bang, Eliazer, Kelly, Agulnick, Smart, Moorman, Kroon, Carpenter, Baetge: "Production of pancreatic hormone-expressing endocrine cells from human embryonic stem cells." in: Nature biotechnology, Vol. 24, Issue 11, pp. 1392-401, (2006) (PubMed).

Inoue, Rudnick, German, Veile, Donis-Keller, Permutt: "Isolation, characterization, and chromosomal mapping of the human Nkx6.1 gene (NKX6A), a new pancreatic islet homeobox gene." in: Genomics, Vol. 40, Issue 2, pp. 367-70, (1997) (PubMed).

Target Details for NKX6-1

Target: NKX6-1 (NK6 Homeobox 1 (NKX6-1))

Alternative Name: Nkx6.1 (NKX6-1 Products)

Synonyms: nkx6a antibody, nkx6.1 antibody, NKX6.1 antibody, NKX6A antibody, zgc:92879 antibody, Nkx6.1 antibody, Nkx61 antibody, Nkx6a antibody, NK6 homeobox 1 antibody, NK6 homeobox 1 L homeolog antibody, nkx6-1 antibody, NKX6-1 antibody, nkx6.1 antibody, nkx6-1.L antibody, Nkx6-1 antibody

Background: Synonyms: Nkx6.1, NKX6-1, NKX6A, Nkx6-1, Nkx6.1, Nkx6a

Molecular Weight: ~44 kDa

Pathways: Dopaminergic Neurogenesis, Positive Regulation of Peptide Hormone Secretion