CCL5 antibody

Catalog No. ABIN2689891

Product Details anti-CCL5 Antibody

Target: CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CCL5 antibody is un-conjugated

Application: Immunocytochemistry (ICC)

Brand: BD Pharmingen™

Characteristics: The 2D5 antibody reacts with human Regulated upon Activation, Normal T cells Expressed and Secreted (RANTES). The immunogen used to generated this hybridoma was recombinant human RANTES protein. Immunocytochemical staining for RANTES. PBMC were isolated from human peripheral blood by density gradient centrifugation and were cultured overnight at 37 °C with LPS (Sigma Cat. No. L-8274, 1 μg / ml) in the presence of BD GolgiStop™ (Cat. No. 554724). The activated cells were harvested and stained by immunocytochemistry using a three-step staining procedure employing a Biotin Goat Anti-Mouse Ig secondary antibody and a Horseradish Peroxidase (HRP) based detection system (Nomarski optics, original magnification 400X).

BD Pharmingen™ Purified Mouse Anti-Human RANTES - Purified - Clone 2D5 - Isotype Mouse IgG1, κ - Reactivity Hu - 0.25 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Recombinant human RANTES protein

Clone: 2D5

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-CCL5 antibody (ABIN2689891)

Sticherling, Küpper, Koltrowitz, Bornscheuer, Kulke, Klinger, Wilhelm, Kameyoshi, Christophers, Schröder: "Detection of the chemokine RANTES in cytokine-stimulated human dermal fibroblasts." in: The Journal of investigative dermatology, Vol. 105, Issue 4, pp. 585-91, (1995) (PubMed).

Hsu, Raine, Fanger: "Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 29, Issue 4, pp. 577-80, (1981) (PubMed).

Target Details for CCL5

Target: CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))

Alternative Name: RANTES (CCL5 Products)

Synonyms: Rantes antibody, Scya5 antibody, D17S136E antibody, RANTES antibody, SCYA5 antibody, SIS-delta antibody, SISd antibody, TCP228 antibody, eoCP antibody, MuRantes antibody, C-C motif chemokine 5-like antibody, C-C motif chemokine ligand 5 antibody, chemokine (C-C motif) ligand 5 antibody, LOC100230935 antibody, CCL5 antibody, Ccl5 antibody

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, Smooth Muscle Cell Migration