CD147 antibody

Catalog No. ABIN2689942

Product Details anti-CD147 Antibody

Target: CD147 (BSG) (Basigin (Ok Blood Group) (BSG))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD147 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), BioImaging (BI)

Brand: BD Pharmingen™

Characteristics: The TRA-1-85 monoclonal antibody (mAb) specifically binds to the high frequency blood group antigen OKa. The OKa blood group antigen is a 35-69 kDa glycoprotein also known as CD147, EMMPRIN, M6, or Basigin. The OKa group is widely expressed on most human tissues including, but not limited to kidney, peripheral blood leukocytes, liver, pancreas, colon, skin, and brain. TRA-1-85 mAb is often used to discern human cells from non-human cells in multiple applications including animal transplantation studies. During development, the purified TRA-1-85 mAb was found to detect OKa blood group antigen by western blot analysis of cellular lysates, by immunofluorescent staining of fixed cells, and by flow cytometric analysis of live cells. Flow cytometric analysis of TRA-1-85 expression on human cervical carcinoma (HeLa cell line). HeLa cells (ATCC® CCL 2™) were stained with Purified Mouse anti-Human TRA-1-85 Antigen monoclonal antibody (solid line histogram) or with Purified Mouse IgG1, κ Isotype Control (Cat. No. 554121, dashed line histogram). The second-step reagent was FITC goat anti-Mouse Ig (Cat. No. 554001). The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of the HeLa cell line. Flow cytometry was performed using a BD LSRFortessa™ Flow Cytometer System. Immunoflourescent staining of Tra-1-85 on human embryonic stem (ES) cells. H9 human ES cells (WiCell, Madison, WI) passage 47 grown in mTESR™1 media (StemCell Technologies) on BD Matrigel™ hESC-qualified Matrix (Cat. No. 354277) were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655). The cells were stained with Purified Mouse anti-Human TRA-1-85 Antigen monoclonal antibody (pseudo-colored green) at 1.2 μg/mL. The second-step reagent was Alexa Fluor® 488 goat anti-mouse Ig (Life Technologies), and cell nuclei were stained with Hoechst (pseudo-colored blue). The images were captured on a BD Pathway™ 435 Cell Analyzer and merged using BD Attovision™ software.

BD Pharmingen™ Purified Mouse anti-Human TRA-1-85 Antigen - Purified - Clone TRA-1-85 - Isotype Mouse IgG1, κ - Reactivity Hu, Ms - 100 μg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Embryonal Carcinoma Cell Line

Clone: TRA-1-85

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-CD147 antibody (ABIN2689942)

Choi, Yu, Smuga-Otto, Salvagiotto, Rehrauer, Vodyanik, Thomson, Slukvin: "Hematopoietic and endothelial differentiation of human induced pluripotent stem cells." in: Stem cells (Dayton, Ohio), Vol. 27, Issue 3, pp. 559-67, (2009) (PubMed).

Olivier, Rybicki, Bouhassira: "Differentiation of human embryonic stem cells into bipotent mesenchymal stem cells." in: Stem cells (Dayton, Ohio), Vol. 24, Issue 8, pp. 1914-22, (2006) (PubMed).

Draper, Pigott, Thomson, Andrews: "Surface antigens of human embryonic stem cells: changes upon differentiation in culture." in: Journal of anatomy, Vol. 200, Issue Pt 3, pp. 249-58, (2002) (PubMed).

Williams, Daniels, Pym, Sheer, Povey, Okubo, Andrews, Goodfellow: "Biochemical and genetic analysis of the OKa blood group antigen." in: Immunogenetics, Vol. 27, Issue 5, pp. 322-9, (1988) (PubMed).

Target Details for CD147

Target: CD147 (BSG) (Basigin (Ok Blood Group) (BSG))

Alternative Name: CD147 (BSG Products)

Synonyms: EMMPRIN antibody, RPE7 antibody, 5F7 antibody, CD147 antibody, M6 antibody, OK antibody, TCSF antibody, AI115436 antibody, AI325119 antibody, HT-7 antibody, 5A11 antibody, CE9 antibody, HT7 antibody, Ox47R antibody, basignin antibody, wu:fb26a05 antibody, wu:fo98e12 antibody, bsg antibody, prso03 antibody, basigin (Ok blood group) antibody, basigin antibody, basigin (Ok blood group) S homeolog antibody, BSG antibody, Bsg antibody, bsg antibody, bsg.S antibody

Background: Synonyms: OKa, CD147, EMMPRIN, Basigin

Pathways: S100 Proteins