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CD25 antibody (Interleukin 2 Receptor, alpha)

Details for Product anti-IL2RA Antibody No. ABIN269014, Supplier: Log in to see
Antigen
  • CD25
  • HEM45
  • IDDM10
  • IL2R
  • TCGFR
  • IL2RAC
  • IL-2RA
  • Il2r
  • Ly-43
Alternatives
anti-Human CD25 antibody for Flow Cytometry
Reactivity
Human
434
327
109
19
10
10
8
5
4
3
3
2
1
Host
Mouse
473
298
89
5
2
Clonality (Clone)
Monoclonal ()
Conjugate
This CD25 antibody is un-conjugated
80
78
60
48
22
15
12
12
10
10
7
6
6
6
4
4
4
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
662
123
117
95
92
65
59
47
40
21
17
15
14
11
10
9
6
6
5
3
2
2
2
1
1
1
Supplier
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Immunogen Tissue / cell preparation (Human). (PHA-activated peripheral blood leucocytes).
Clone MEM-181
Isotype IgG1
Specificity IL2RA/CD25 antigen (human).
Purification Affinity purified
Alternative Name CD25 / IL2RA (IL2RA Antibody Abstract)
Background The antibody MEM-181 reacts with CD25 (Interleukin-2 receptor alpha chain), a 55 kDa type I transmembrane glycoprotein expressed on activated B and T lymphocytes, activated monocytes/macrophages and on CD4+ T lymphocytes (T regulatory cells); it is lost on resting B and T lymphocytes. HLDA VI; WS Code NL N-L024
Gene ID 3559
Research Area CD Antigens, Surface Receptors of Immune Cells, Cytokines, Receptors, Inflammation
Pathways JAK-STAT Signaling
Application Notes Flow Cytometry Use at an assay dependant concentration. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Recommended dilutions: Flow Cytometry 1:10-1:1000
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer PBS [pH 7.4], Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C
Storage Comment 4 °C short term. Aliquot and store at -20 °C long term.
Product cited in: Michaëlsson, Mold, McCune et al.: "Regulation of T cell responses in the developing human fetus." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 176, Issue 10, pp. 5741-8, 2006 (PubMed).