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Discs, Large Homolog 1 (Drosophila) (DLG1) (AA 115-133) antibody

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Synonyms DLG1, Dlgh1, SAP97, B130052P05Rik, E-dlg/SAP97, SAP-97, mKIAA4187, DLGH1, dJ1061C18.1.1, hdlg
AA 115-133
(19), (12), (6), (4), (4), (3), (2), (1), (1), (1), (1), (1)
Rat (Rattus)
(55), (41), (34), (2), (1), (1), (1), (1)
(37), (16), (14), (1)
(2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro))
(64), (29), (25), (20), (5), (4), (3), (3), (2), (2), (2), (1), (1)
Pubmed 4 references available
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Quantity 0.1 mL
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Immunogen Synthetic peptide: V(115)LPSERISPQVPNEVLGPE(133).Immunizing peptide corresponds to amino acids 115-133 from rat SAP 97. This sequence is 84% and 74% conserved in mouse and human SAP 97, respectively.
Isotype IgG
Specificity Detects SAP 97 from rat tissues. No cross-reactivity to other proteins, including synapse-associated protein family members, has been observed.
Cross-Reactivity Rat (Rattus)
Cross-Reactivity (Details) Expected to cross-react with Human,Mouse due to sequence identity. Not yet tested in other species.
Purification IgG purified
Alternative Name DLG1 (DLG1 Antibody Abstract)
Background Synapse-associated protein (SAP) 97 is a 97 kDa membraneassociated synapseprotein with extensive sequence homology to the PSD 95 family of proteins that facilitateion channel clustering at synaptic terminal. SAP97 consists of three 90 amino acid PDZdomains at its amino terminus, followed by a Src homology (SH) 3 domain, and a regionwith homology to guanylate kinases (GK). In addition to the CNS, SAP97 is alsodetected at the basal lateral membrane between a variety of epithelial cells. SAP97 isrecruited to the submembranous cortical cytoskeleton at cell-cell contact sites as part ofthe E-cadherin complex. Co-expression of SAP97 and K+ channel results in thecolocalization of these proteins in large round intracellular aggregates. SAP 97 has beenshown to have functional homology to Drosophila tumor suppressor protein (DlgA), aPSD 95 related protein involved in maintaining integrity of epithelial and neuromuscularjunctions. Alternate Names: anti-Discs large homolog 1 antibody, anti-Disks large homolog 1 antibody,anti-dJ1061C18.1.1 antibody, anti-DKFZp761P0818 antibody, anti-DLG 1 antibody,anti-DLG1 antibody, anti-DLGH 1 antibody, anti-DLGH1 antibody, anti-HDLG antibody,anti-Presynaptic protein SAP97 antibody, anti-SAP 97 antibody, anti-sap97 antibody,anti-Synapse associated protein 97 antibody.
Gene Symbol: DLG1
Gene ID 1739, 25252
Application Notes Immunohistochemistry: use at a dilution of 1/1000. Western Blot: use at a dilution of 1/1000.Not tested in other species. Optimal dilution / concentration should be determined by the end user.By Western blot, this antibody detects an ~140 kDa protein representing SAP 97 from rat brain extract. Immunohistochemical staining of SAP 97 in rat hippocampus results in intense neuronal cell body and dendrite staining.
Recommended dilutions: Immunohistochemistry-Frozen 1:10-1:2000, Western Blot 1:100-1:2000, Immunohistochemistry 1:10-1:2000
Restrictions For Research Use only
Format Liquid
Buffer PBS and 1 mg/ml BSA, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Aliquot and store at -20 °C or -80 °C.
Product cited in: Gardner, Naren, Bahouth: "Assembly of an SAP97-AKAP79-cAMP-dependent protein kinase scaffold at the type 1 PSD-95/DLG/ZO1 motif of the human beta(1)-adrenergic receptor generates a receptosome involved in receptor recycling and networking." in: The Journal of biological chemistry, Vol. 282, Issue 7, pp. 5085-99, 2007 (PubMed).

Background publications Gomperts: "Clustering membrane proteins: It's all coming together with the PSD-95/SAP90 protein family." in: Cell, Vol. 84, Issue 5, pp. 659-62, 1996 (PubMed).

Mueller, Kistner, Veh et al.: "Molecular characterization and spatial distribution of SAP97, a novel presynaptic protein homologous to SAP90 and the Drosophila discs-large tumor suppressor protein." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 15, Issue 3 Pt 2, pp. 2354-66, 1995 (PubMed).

Cho, Hunt, Kennedy: "The rat brain postsynaptic density fraction contains a homolog of the Drosophila discs-large tumor suppressor protein." in: Neuron, Vol. 9, Issue 5, pp. 929-42, 1992 (PubMed).

Catalog No. ABIN269417
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