You are viewing an incomplete version of our website. Please click to reload the website as full version.

KI-67 antibody (Antigen Identified By Monoclonal antibody Ki-67) (C-Term)

Details for Product anti-MKI67 Antibody No. ABIN269440, Supplier: Log in to see
Antigen
  • D630048A14Rik
  • Ki-67
  • Ki67
  • kia
  • KIA
  • MGC132156
  • MIB-1
  • MKI67
  • mki67
Epitope
C-Term
55
48
46
18
16
15
13
12
8
5
4
4
4
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
387
101
64
15
7
3
2
1
Host
Rabbit
215
133
32
11
3
Clonality (Clone)
Monoclonal ()
Conjugate
This KI-67 antibody is un-conjugated
14
9
8
8
8
6
5
4
4
4
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)
176
144
140
93
82
70
65
65
10
10
7
6
3
3
2
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Immunogen A synthetic peptide from C-terminus of human Ki-67.
Clone SP6
Isotype IgG
Specificity Ki67 (SP6)
Cross-Reactivity (Details) Mouse reactivity reported in scientific literature (PMID: 22020958).
Purification Tissue culture supernatant
Alternative Name Ki-67/MKI67 (MKI67 Antibody Abstract)
Background Gene Symbol: MKI67
Gene ID 4288, 291234
Application Notes Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Paraffin 1:100-1:200, Immunohistochemistry-Frozen 1:10-1:500
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Immunohistochemistry-Paraffin Protocol Specific for NB600-1252: Ki67 Antibody (SP6)Materials1) 1 Phosphate buffered saline (pH 7.6): NaCl 137mmol/L, KCl 2.7mmol/L, Na2HPO4 4.3mmol/L, KH2PO4 1.4 mmol/L2) Citrate buffer, 0.01 M, pH6.0, Sodium Citrate 3g, Citric acid 0.4g3) 3 % Hydrogen peroxide4) Primary antibody5) Blocking serum (normal serum)6) Biotinylated secondary antibody7) DAB staining kitMethods
1. Dewax and hydration of slides using xylene and EtOH:Dry slides for 20 min in a 60 C ovenAdd Xylene, 2 x 10 min100 %, 95 %, 80 %, and 70 % EtOH, 5 min each EtOH concentration Rinse in PBS, 5'2 Antigen retrieval method (only for paraffin slides)1a. High-pressure antigen retrieval procedure (recommended method)Place slides in a glass slide holder (ensure that the slide holder is completely filled with slides, slides without sections if necessary, to ensure even heating. The entire slide holder is immersed in 1000 mL of Citrate buffer (0.01M, pH6.
0) within a pressure cookerOnce steam is produced, and ONLY when steam is visible, from the pressure cooker (usually 15-20 min), the required high-pressure will have been reached, and slides will be incubated for 2 min.Turn off heat, and allow buffer and slides to cool to room temperatureSlides are then rinsed in PBS for 5 minutes
. Add 3 % hydrogen peroxide solution, 10'at RT, then PBS, 3X5'
. Normal blocking serum, 20'at RT
. Incubate with Primary Ab, 4C overnight or 1.5 hours at 37C
. Rinse with PBS, 3 X 5' each rinse
. Add Biotin-conjugated second antibody, 10'at RT
. Rinse with PBS, 3 X 5' each rinse
. Add Streptavidin-Peroxidase, 10'at RT
. Rinse with PBS, 3 X 5' each rinse
. Staining with DAB solution, 2-5'under microscope
. Stop the reaction by washing in tap water
. Counterstain in Haematoxylin for 3-5 minutes
. 75 %, 80 %, 95 % and 100 % ethanol, 5x2', xylene 2 x 10'
Restrictions For Research Use only
Format Liquid
Buffer PBS, BSA
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-KI-67 antibody (Antigen Identified By Monoclonal antibody Ki-67) (C-Term) (ABIN269440) Immunohistochemistry-Paraffin: Ki-67/MKI67 Antibody (SP6) [ABIN269440] - Formalin fix...
Product cited in: Falkenberg, Höfig, Rosemann, Szumielewski, Richter, Schorpp, Hadian, Aubele, Atkinson, Anastasov: "Three-dimensional microtissues essentially contribute to preclinical validations of therapeutic targets in breast cancer." in: Cancer medicine, Vol. 5, Issue 4, pp. 703-10, 2016 (PubMed). (Sample species: Human). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Rath, Deb, Sengupta, Kari, Kar, Parbin, Pradhan, Patra: "Silencing of ZRF1 impedes survival of estrogen receptor positive MCF-7 cells and potentiates the effect of curcumin." in: Tumour biology, Vol. 37, Issue 9, pp. 12535-12546, 2016 (PubMed). (Sample species: Human). Further details: Immunohistochemistry

Kalliokoski, Piqueras, Frías, Sulic, Määttä, Kähkönen, Viiri, Huhtala, Pasternack, Laurila, Sblattero, Korponay-Szabó, Mäki, Caja, Kaukinen, Lindfors: "Transglutaminase 2-specific coeliac disease autoantibodies induce morphological changes and signs of inflammation in the small-bowel mucosa of mice." in: Amino acids, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunohistochemistry

Sumis, Cook, Andrade, Hu, Kidney, Zhang, Kim, Carney, Nguyen, Yu, Bouker, Cruz, Clarke, Hilakivi-Clarke: "Social isolation induces autophagy in the mouse mammary gland: link to increased mammary cancer risk." in: Endocrine-related cancer, Vol. 23, Issue 10, pp. 839-56, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunohistochemistry

Du, Bondarenko, Wang, Rouabhia, Zhang: "Ex Vivo Assay of Electrical Stimulation to Rat Sciatic Nerves: Cell Behaviors and Growth Factor Expression." in: Journal of cellular physiology, Vol. 231, Issue 6, pp. 1301-12, 2016 (PubMed). (Sample species: Rat (Rattus)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Migita, Yoshitake, Tange, Choijookhuu, Hishikawa: "Hyperbaric Oxygen Therapy Suppresses Apoptosis and Promotes Renal Tubular Regeneration After Renal Ischemia/Reperfusion Injury in Rats." in: Nephro-urology monthly, Vol. 8, Issue 1, pp. e34421, 2016 (PubMed). Method employed by authors: Western Blotting (WB)

Ueha, Ueha, Sakamoto, Kanaya, Suzukawa, Nishijima, Kikuta, Kondo, Matsushima, Yamasoba: "Cigarette Smoke Delays Regeneration of the Olfactory Epithelium in Mice." in: Neurotoxicity research, Vol. 30, Issue 2, pp. 213-24, 2016 (PubMed). Method employed by authors: Western Blotting (WB)

Ueha, Ueha, Kondo, Sakamoto, Kikuta, Kanaya, Nishijima, Matsushima, Yamasoba: "Damage to Olfactory Progenitor Cells Is Involved in Cigarette Smoke-Induced Olfactory Dysfunction in Mice." in: The American journal of pathology, Vol. 186, Issue 3, pp. 579-86, 2016 (PubMed).

Orticello, Fiore, Totta, Desideri, Barisic, Passeri, Lenzi, Rosa, Orlandi, Maiato, Del Bufalo, Degrassi: "N-terminus-modified Hec1 suppresses tumour growth by interfering with kinetochore-microtubule dynamics." in: Oncogene, Vol. 34, Issue 25, pp. 3325-35, 2015 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Papazoglou, Jean, Gertler, Taouis, Vacher: "Hippocampal GSK3β as a Molecular Link Between Obesity and Depression." in: Molecular neurobiology, Vol. 52, Issue 1, pp. 363-74, 2015 (PubMed).

Kalliokoski, Caja, Frias, Laurila, Koskinen, Niemelä, Mäki, Kaukinen, Korponay-Szabó, Lindfors: "Injection of celiac disease patient sera or immunoglobulins to mice reproduces a condition mimicking early developing celiac disease." in: Journal of molecular medicine (Berlin, Germany), Vol. 93, Issue 1, pp. 51-62, 2015 (PubMed).

Schevzov, Kee, Wang, Sequeira, Hook, Coombes, Lucas, Stehn, Musgrove, Cretu, Assoian, Fath, Hanoch, Seger, Pleines, Kile, Hardeman, Gunning: "Regulation of cell proliferation by ERK and signal-dependent nuclear translocation of ERK is dependent on Tm5NM1-containing actin filaments." in: Molecular biology of the cell, Vol. 26, Issue 13, pp. 2475-90, 2015 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Cage, Chanthery, Chesler, Grimmer, Knight, Shokat, Weiss, Gustafson: "Downregulation of MYCN through PI3K Inhibition in Mouse Models of Pediatric Neural Cancer." in: Frontiers in oncology, Vol. 5, pp. 111, 2015 (PubMed). (Sample species: Human). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Kim, Wong, Amarnani, Bigger-Allen, Hu, Marko, Eliott, Shah, McGuone, Stemmer-Rachamimov, Gai, DAmore, Arboleda-Velasquez: "Characterization of cells from patient-derived fibrovascular membranes in proliferative diabetic retinopathy." in: Molecular vision, Vol. 21, pp. 673-87, 2015 (PubMed). (Sample species: Human). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Vesci, Carollo, Roscilli, Aurisicchio, Ferrara, Spagnoli, De Santis: "Trastuzumab and docetaxel in a preclinical organotypic breast cancer model using tissue slices from mammary fat pad: Translational relevance." in: Oncology reports, Vol. 34, Issue 3, pp. 1146-52, 2015 (PubMed). (Sample species: Human). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Vesci, Carollo, Roscilli, Aurisicchio, Ferrara, Spagnoli, De Santis: "[Corrigendum] Trastuzumab and docetaxel in a preclinical organotypic breast cancer model using tissue slices from mammary fat pad: Translational relevance." in: Oncology reports, Vol. 35, Issue 1, pp. 602, 2015 (PubMed).

Tukler Henriksson, Coursey, Corry, De Paiva, Pflugfelder: "IL-13 Stimulates Proliferation and Expression of Mucin and Immunomodulatory Genes in Cultured Conjunctival Goblet Cells." in: Investigative ophthalmology & visual science, Vol. 56, Issue 8, pp. 4186-97, 2015 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence

Hua, Schindler, McQuail, Forbes, Riddle: "Regionally distinct responses of microglia and glial progenitor cells to whole brain irradiation in adult and aging rats." in: PLoS ONE, Vol. 7, Issue 12, pp. e52728, 2013 (PubMed). (Sample species: Rat (Rattus)). Further details: Immunohistochemistry (Frozen Sections)

Vulpio, Bossola, Di Stasio, Tazza, Silvestri, Fadda: "Histology and immunohistochemistry of the parathyroid glands in renal secondary hyperparathyroidism refractory to vitamin D or cinacalcet therapy." in: European journal of endocrinology, Vol. 168, Issue 6, pp. 811-9, 2013 (PubMed). (Sample species: Human). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Pusic, Kraig: "Youth and environmental enrichment generate serum exosomes containing miR-219 that promote CNS myelination." in: Glia, Vol. 62, Issue 2, pp. 284-99, 2013 (PubMed). (Sample species: Rat (Rattus)). Further details: Immunohistochemistry (Frozen Sections)