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ATG7 Autophagy Related 7 Homolog (S. Cerevisiae) (ATG7) (AA 558-569) antibody

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Synonyms AO090001000744, atg7, APG7-LIKE, APG7L, GSA7, 1810013K23Rik, Agp7, Apg7l, Atg7l, ATAPG7, ATATG7, ATG7, AUTOPHAGY 7, AUTOPHAGY-RELATED 7, K15I22.10, K15I22_10
AA 558-569
(30), (15), (15), (14), (13), (8), (7), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
(145), (40), (24)
(139), (10), (3)
(4), (4), (4), (4), (4), (4), (1)
Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)
(116), (61), (55), (33), (22), (17), (10), (7), (6), (3), (3), (2), (1), (1)
Pubmed 1 reference available
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Quantity 50 μg
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Immunogen This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to aa 558-569 of Human APG7 protein.
Isotype IgG
Specificity Reactivity occurs against human Apg7 protein. However, BLAST analysis indicates 100% homology (12/12) for this protein from human, chimpanzee, mouse, rat, dog, and chicken sources. The core amino acids of the immunogen, G-D-S-T-R-D-R-T are 100% identical (8/8) in D. melanogaster. Reactivity with Apg7 proteins from other sources is not known.
Cross-Reactivity (Details) This product is believed to react with chimpanzee, mouse, rat, dog,chicken.
Purification Affinity purified
Alternative Name APG7L / ATG7 (ATG7 Antibody Abstract)
Background Apg7 is the human gene product similar in homology to Pichia pastoris GSA7 andSaccharomyces cerevisiae Apg7. In the yeast, the protein appears to be required forfusion of peroxisomal andvaculuolar membranes. The protein shows homlogy to the ATP-binding and catalyticsites of the E1 ubiquitin activatingenzymes. Alternate Names: anti-APG7 antibody, anti-ATG7 antibody, anti-Autophagy protein 7 antibody,anti-Autophagy 7-like antibody, anti-gsa7 antibody.
Gene Symbol: ATG7
Gene ID 10533
Application Notes This affinity purified antibody has been tested for use in ELISA and by western blot. Expect a band approximately 78 kDa in size corresponding - human Apg7 by western blotting in the appropriate cell lysate or extract. Human Apg7 has been reported - form a homodimer at approximately 160 kDa.
Recommended dilutions: ELISA 1:15000-1:75000, Immunocytochemistry/Immunofluorescence 1:10-1:2000, Western Blot 1:500-1:2000
Restrictions For Research Use only
Buffer 0.02 M Potassium Phosphate, 0.02 M Sodium Chloride, pH 7.2, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Aliquot and store at -20 °C or -80 °C.
Product cited in: Park, Lee, Kim et al.: "A human scFv antibody against TRAIL receptor 2 induces autophagic cell death in both TRAIL-sensitive and TRAIL-resistant cancer cells." in: Cancer research, Vol. 67, Issue 15, pp. 7327-34, 2007 (PubMed).

Catalog No. ABIN269462
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