ATG7 Autophagy Related 7 Homolog (S. Cerevisiae) (ATG7) (AA 558-569) antibody

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Antigen
  • AO090001000744
  • atg7
  • APG7-LIKE
  • APG7L
  • GSA7
  • 1810013K23Rik
  • Agp7
  • Apg7l
  • Atg7l
  • ATAPG7
  • ATATG7
  • ATG7
  • AUTOPHAGY 7
  • AUTOPHAGY-RELATED 7
  • K15I22.10
  • K15I22_10
  • autophagy-related protein 7
  • Autophagy-related protein 7
  • autophagy related 7
  • similar to S. cerevisiae ATG7 (YHR171W) protein-conjugating enzyme of the Apg12p-Apg5p conjugation pathway of autophagy
  • ubiquitin-like modifier-activating enzyme atg7
  • ANI_1_3368024
  • AOR_1_1338164
  • PTRG_02236
  • PAAG_00287
  • PITG_12537
  • atg7
  • ATG7
  • Atg7
  • APG7
Alternatives
anti-Mouse (Murine) ATG7 Autophagy Related 7 Homolog (S. Cerevisiae) antibody for ELISA
Epitope
AA 558-569
44
37
14
14
11
8
8
7
7
6
5
5
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
197
109
72
2
1
1
1
1
1
1
1
Host
Rabbit
180
18
4
Clonality
Polyclonal
Conjugate
Un-conjugated
6
6
6
6
6
6
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA, Western Blotting (WB)
159
69
59
39
36
31
24
7
6
4
3
1
1
Options
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Immunogen This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to aa 558-569 of Human APG7 protein.
Specificity Reactivity occurs against human Apg7 protein. The core amino acids of the immunogen, G-D-S-T-R-D-R-T are 100 % identical (8/8) in D. melanogaster. Reactivity with Apg7 proteins from other sources is not known.
Purification Immunogen affinity purified
Alternative Name ATG7 (ATG7 Antibody Abstract)
Background Gene Symbol: ATG7
Gene ID 10533
Pathways Response to Water Deprivation, Autophagy
Application Notes Western Blot 1:500-1:2000, ELISA 1:300000 - 1:400000, Immunocytochemistry/Immunofluorescence 1:10-1:2000This affinity purified antibody has been tested for use in ELISA and by western blot. Expect a band approximately 78 kDa in size corresponding - human Apg7 by western blotting in the appropriate cell lysate or extract. Human Apg7 has been reported - form a homodimer at approximately 160 kDa.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Buffer contains: 0.01 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -80 °C,-20 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Product cited in: Rashid, Runci, Polletta, Carnevale, Morgante, Foglio, Arcangeli, Sansone, Russo, Tafani: "Muscle LIM protein/CSRP3: a mechanosensor with a role in autophagy." in: Cell death discovery, Vol. 1, pp. 15014, 2016 (PubMed). Method employed by authors: Western Blotting (WB)

Razi, Chan, Tooze: "Early endosomes and endosomal coatomer are required for autophagy." in: The Journal of cell biology, Vol. 185, Issue 2, pp. 305-21, 2009 (PubMed).

Lee, Gao: "Inhibition of autophagy induction delays neuronal cell loss caused by dysfunctional ESCRT-III in frontotemporal dementia." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 29, Issue 26, pp. 8506-11, 2009 (PubMed).

Park, Lee, Kim, Lee, Lee, Kim, Jang, Choi, Kwon, Kim: "A human scFv antibody against TRAIL receptor 2 induces autophagic cell death in both TRAIL-sensitive and TRAIL-resistant cancer cells." in: Cancer research, Vol. 67, Issue 15, pp. 7327-34, 2007 (PubMed).