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ATG7 Autophagy Related 7 Homolog (S. Cerevisiae) (ATG7) (AA 558-569) antibody

Details for Product No. ABIN269462, Supplier: Log in to see
Antigen
  • 1810013K23Rik
  • Agp7
  • AO090001000744
  • APG7-LIKE
  • Apg7l
  • APG7L
  • ATAPG7
  • ATATG7
  • atg7
  • ATG7
  • Atg7l
  • AUTOPHAGY-RELATED 7
  • AUTOPHAGY 7
  • GSA7
  • K15I22.10
  • K15I22_10
Epitope
AA 558-569
30
19
15
14
13
8
7
5
4
3
3
3
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human
154
49
29
Host
Rabbit
145
12
4
Clonality
Polyclonal
Conjugate
Un-conjugated
4
4
4
4
4
4
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA, Western Blotting (WB)
124
65
59
37
23
17
13
6
6
3
3
2
1
1
Supplier
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Immunogen This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to aa 558-569 of Human APG7 protein.
Isotype IgG
Specificity Reactivity occurs against human Apg7 protein. The core amino acids of the immunogen, G-D-S-T-R-D-R-T are 100 % identical (8/8) in D. melanogaster. Reactivity with Apg7 proteins from other sources is not known.
Cross-Reactivity (Details) This product is believed to react with chimpanzee, mouse, rat, dog,chicken.
Purification Immunogen affinity purified
Alternative Name ATG7 (ATG7 Antibody Abstract)
Background Gene Symbol: ATG7
Application Notes Western Blot 1:500-1:2000, ELISA 1:300000 - 1:400000, Immunocytochemistry/Immunofluorescence 1:10-1:2000
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2
Buffer contains: 0.01 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C,-80 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Product cited in: Rashid, Runci, Polletta, Carnevale, Morgante, Foglio, Arcangeli, Sansone, Russo, Tafani: "Muscle LIM protein/CSRP3: a mechanosensor with a role in autophagy." in: Cell death discovery, Vol. 1, pp. 15014, 2016 (PubMed). Method employed by authors: Western Blotting (WB)

Razi, Chan, Tooze: "Early endosomes and endosomal coatomer are required for autophagy." in: The Journal of cell biology, Vol. 185, Issue 2, pp. 305-21, 2009 (PubMed).

Lee, Gao: "Inhibition of autophagy induction delays neuronal cell loss caused by dysfunctional ESCRT-III in frontotemporal dementia." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 29, Issue 26, pp. 8506-11, 2009 (PubMed).

Park, Lee, Kim, Lee, Lee, Kim, Jang, Choi, Kwon, Kim: "A human scFv antibody against TRAIL receptor 2 induces autophagic cell death in both TRAIL-sensitive and TRAIL-resistant cancer cells." in: Cancer research, Vol. 67, Issue 15, pp. 7327-34, 2007 (PubMed).