Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (N-Term) antibody (Biotin)

Details for Product No. ABIN269556
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Antigen
Synonyms Map1lc3, zgc:56434, wu:fb60g11, map1lc3b, MGC76283, MAP1LC3B, ATG8F, LC3B, MAP1A/1BLC3, MAP1LC3B-a, 1010001C15Rik, Atg8, LC3b, Mpl3, zbs559
Epitope
N-Term
(26), (11), (9), (9), (6), (6), (6), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(117), (44), (35), (24), (24), (14), (13), (4), (2), (1)
Host
Rabbit
(107), (14), (1)
Clonality
Polyclonal
Conjugate
Biotin
(9), (7), (7), (7), (6), (5), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application
Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(64), (55), (32), (26), (23), (20), (20), (18), (12), (5), (2), (1), (1)
Pubmed 2 references available
Quantity 0.1 mL
Shipping to United States (Change)
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Catalog No. ABIN269556
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Immunogen A synthetic peptide made to the N-terminal region of the human LC3B protein.
Cross-Reactivity (Details) Zebrafish reactivity reported in scientific literature (PMID: 23724125). Canine,primate reactivity reported in scientific literature (PMID: 24027311) Other species have not been tested.
Purification affinity purified
Alternative Name LC3B
Background Autophagy is a process of intracellular bulk degradation in which cytoplasmiccomponents, including organelles, are sequestered within double-membrane vesiclesthat deliver the contents to the lysosome/vacuole for degradation. Duringmacroautophagy, the sequestering vesicles, termed autophagosomes, fuse with thelysosome or vacuole resulting in the delivery of an inner vesicle (autophagic body) intothe lumen of the degradative compartment. There are 16 proteins participating in theautophagy pathway in humans. The autophagy protein LC3, a mammalian homologueof Atg8, was originally identified as microtubule-associated protein 1 light chain 3. It is acomponent of both the MAP1A and MAP1B microtubule-binding domains and theheavy-chain independent regulation of LC3 expression may modify MAP1microtubule-binding activity during development. LC3 is the only known mammalianprotein identified that stably associates with the autophagosome membranes. LC3-I is cytosolic and LC3-II ismembrane bound and enriched in the autophagic vacuole fraction. The detection ofLC3-I to LC3-II conversion is a useful and sensitive marker for distinguishing autophagyin mammalian cells. Alternate Names: anti-Microtubule-associated protein 1 light chain 3 beta antibody, anti-MAP1A/MAP1BLC3 B antibody, anti-MAP1A/1B light chain 3 B antibody, anti-MAP1 light chain 3-likeprotein 2 antibody, anti-Autophagy-related protein LC3 B antibody,anti-Autophagy-related ubiquitin-like modifier LC3 B antibody, anti-LC3-II antibody.
Gene Symbol: MAP1LC3B
Gene ID 81631, 67443
UniProt Q9GZQ8
Research Area Cancer, Autophagy, Cytoskeleton
Application Notes This LC3 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry-frozen sections, Flow Cytometry and Western Blot where bands are seen ~17 and 19 kDa corresponding to LC3-II and LC3-I. Electron Microscopy was reported in scientific literature.
Recommended dilutions: Immunocytochemistry/Immunofluorescence 1:2000, Immunohistochemistry 1:2000, Immunohistochemistry-Paraffin 1:2000, Western Blot 1:3000
Restrictions For Research Use only
Format Liquid
Concentration 1.00 mg/mL
Buffer PBS
Preservative Without preservative
Storage 4 °C
Storage Comment 4 °C in the dark
General Aoki, Takada, Kondo et al.: "Evidence that curcumin suppresses the growth of malignant gliomas in vitro and in vivo through induction of autophagy: role of Akt and extracellular signal-regulated kinase signaling pathways." in: Molecular pharmacology, Vol. 72, Issue 1, pp. 29-39, 2007 (PubMed).

Pyo, Nah, Kim et al.: "Compensatory activation of ERK1/2 in Atg5-deficient mouse embryo fibroblasts suppresses oxidative stress-induced cell death." in: Autophagy, Vol. 4, Issue 3, pp. 315-21, 2008 (PubMed).

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