Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) antibody

Details for Product No. ABIN269557
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms ATG8F, LC3B, MAP1A/1BLC3, MAP1LC3B-a, Map1lc3, Mpl3, zbs559, 1010001C15Rik, Atg8, LC3b, zgc:56434, wu:fb60g11, map1lc3b, MGC76283, MAP1LC3B
Reactivity
Human
(85), (49), (39), (25), (25), (14), (14), (4), (1), (1), (1), (1)
Host
Rabbit
(73), (14)
Clonality
Polyclonal
Conjugate
Un-conjugated
(4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB)
(53), (27), (22), (20), (19), (17), (15), (8), (7), (4), (2), (1), (1)
Pubmed 2 references available
Catalog no. ABIN269557
Quantity 0.1 mL
Price
Contact our Customer Service for availability and price in your country.
Shipping to United States (Change)
Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen A synthetic peptide made to the N-terminal region of the human LC3, isoform B protein.
Specificity This antibody is specific for the B isoform of LC3. Species Reactivity: This antibody reacts with the human and mouse proteins. Other species have not beentested. Localization: LC3 I: cytoplasmic, LC3 II: Intracytoplasmic membrane, lipid anchor. Cytoplasmicvesicle, autophagosome, autophagosomal membrane, lipid anchor.
Cross-Reactivity Human, Mouse (Murine)
Purification Serum
Alternative Name LC3B
Background Autophagy is a process of intracellular bulk degradation in which cytoplasmiccomponents, including organelles, are sequestered within double-membrane vesiclesthat deliver the contents to the lysosome/vacuole for degradation. Duringmacroautophagy, the sequestering vesicles, termed autophagosomes, fuse with thelysosome or vacuole resulting in the delivery of an inner vesicle (autophagic body) intothe lumen of the degradative compartment. There are 16 proteins participating in theautophagy pathway in humans. The autophagy protein LC3, a mammalian homologueof Atg8, was originally identified as microtubule-associated protein 1 light chain 3. It is acomponent of both the MAP1A and MAP1B microtubule-binding domains and theheavy-chain independent regulation of LC3 expression may modify MAP1microtubule-binding activity during development. LC3 is the only known mammalianprotein identified that stably associates with the autophagosome membranes. LC3-I is cytosolic and LC3-II ismembrane bound and enriched in the autophagic vacuole fraction. The detection ofLC3-I to LC3-II conversion is a useful and sensitive marker for distinguishing autophagyin mammalian cells. Alternate Names: anti-Microtubule-associated protein 1 light chain 3 beta antibody, anti-MAP1A/MAP1BLC3 B antibody, anti-MAP1A/1B light chain 3 B antibody, anti-MAP1 light chain 3-likeprotein 2 antibody, anti-Autophagy-related protein LC3 B antibody,anti-Autophagy-related ubiquitin-like modifier LC3 B antibody, anti-LC3-II antibody.
Gene ID 81631, 67443
Research Area Cancer, Autophagy, Cytoskeleton
Application Notes Suggested working dilutions: immunohistochemistry 1:2000,immunofluorescence 1:2000,Western Blot 1:3000,Immunocytochemistry 1:2000
Restrictions For Research Use only
Format Liquid
Buffer Tris-glycine, 150mM NaCl
Storage 4 °C
General Aoki, Takada, Kondo et al.: "Evidence that curcumin suppresses the growth of malignant gliomas in vitro and in vivo through induction of autophagy: role of Akt and extracellular signal-regulated kinase signaling pathways." in: Molecular pharmacology, Vol. 72, Issue 1, pp. 29-39, 2007 (PubMed).

Pyo, Nah, Kim et al.: "Compensatory activation of ERK1/2 in Atg5-deficient mouse embryo fibroblasts suppresses oxidative stress-induced cell death." in: Autophagy, Vol. 4, Issue 3, pp. 315-21, 2008 (PubMed).

Hosts (73), (14)
Reactivities (85), (49), (39), (25), (25), (14), (14), (4), (1), (1), (1), (1)
Applications (53), (27), (22), (20), (19), (17), (15), (8), (7), (4), (2), (1), (1)
Conjugates (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Epitopes (17), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
back to top