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Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (N-Term) antibody

Details for Product No. ABIN269559, Supplier: Log in to see
Antigen
  • 1010001C15Rik
  • Atg8
  • ATG8F
  • LC3b
  • LC3B
  • MAP1A/1BLC3
  • Map1lc3
  • map1lc3b
  • MAP1LC3B
  • MAP1LC3B-a
  • MGC76283
  • Mpl3
  • wu:fb60g11
  • zbs559
  • zgc:56434
Epitope
N-Term
68
28
22
20
16
12
11
11
8
8
7
6
6
6
6
4
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
224
87
58
24
24
19
18
17
8
8
5
3
1
Host
Rabbit
209
18
1
Clonality
Polyclonal
Conjugate
Un-conjugated
15
14
12
12
11
10
4
4
4
4
3
3
3
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunoelectron Microscopy (IEM), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
116
112
78
78
66
48
46
23
15
11
10
6
4
3
1
Supplier
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Immunogen A synthetic peptide made to the N-terminal region of the human LC3B protein.
Cross-Reactivity (Details) Zebrafish reactivity reported in scientific literature (PMID: 23724125). Canine,primate reactivity reported in scientific literature (PMID: 24027311) Other species have not been tested.
Purification affinity purified
Alternative Name LC3B/MAP1LC3B (MAP1LC3B Antibody Abstract)
Background Autophagy is a process of intracellular bulk degradation in which cytoplasmiccomponents, including organelles, are sequestered within double-membrane vesiclesthat deliver the contents to the lysosome/vacuole for degradation. Duringmacroautophagy, the sequestering vesicles, termed autophagosomes, fuse with thelysosome or vacuole resulting in the delivery of an inner vesicle (autophagic body) intothe lumen of the degradative compartment. There are 16 proteins participating in theautophagy pathway in humans. The autophagy protein LC3, a mammalian homologueof Atg8, was originally identified as microtubule-associated protein 1 light chain 3. It is acomponent of both the MAP1A and MAP1B microtubule-binding domains and theheavy-chain independent regulation of LC3 expression may modify MAP1microtubule-binding activity during development. LC3 is the only known mammalianprotein identified that stably associates with the autophagosome membranes. LC3-I is cytosolic and LC3-II ismembrane bound and enriched in the autophagic vacuole fraction. The detection ofLC3-I to LC3-II conversion is a useful and sensitive marker for distinguishing autophagyin mammalian cells. Alternate Names: anti-Microtubule-associated protein 1 light chain 3 beta antibody, anti-MAP1A/MAP1BLC3 B antibody, anti-MAP1A/1B light chain 3 B antibody, anti-MAP1 light chain 3-likeprotein 2 antibody, anti-Autophagy-related protein LC3 B antibody,anti-Autophagy-related ubiquitin-like modifier LC3 B antibody, anti-LC3-II antibody.
Gene Symbol: MAP1LC3B
Gene ID 81631, 67443
UniProt Q9GZQ8
Research Area Cancer, Autophagy, Cytoskeleton
Application Notes This LC3 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry-frozen sections, Flow Cytometry and Western Blot where bands are seen ~17 and 19 kDa corresponding to LC3-II and LC3-I. Electron Microscopy was reported in scientific literature.
Recommended dilutions: Electron Microscopy, Flow Cytometry 1:200, Immunocytochemistry/Immunofluorescence 0.1-2 µg/mL, Immunohistochemistry, Immunohistochemistry-Frozen 1:400, Immunohistochemistry-Paraffin 1:200-1:400, Western Blot 1:1000
Protocol Protocol specific for LC3B Antibody Fluorescent Staining
1. After washing sample(s) in PBS, fix for 20 minutes at room temperature.
. Wash sample(s) in PBS 3x 3 minutes, each.
. Block sample(s) with 5 % NGS (keep Triton X-100 at 0.1 %) for 60 minutes at room temperature
. Prepare primary anti-LC3B solution during this time at a 1:5,000 dilution with 1 %NGS (keep Triton X-100 at 0.1 %).
. Incubate sample(s) in the primary Ab overnight at 4C in a humidified containerNote: If thick tissue samples, Ab must be prepared in 0.5 % Triton X-100/PBSDay
. Wash sample(s) in PBS 3x 5 minutes with wells on. Prepare Alexa-fluor antibodies (Goat anti-Rabbit IgG H+L) as secondary Ab with 1 % NGS (keep Triton X-100 at 0.1 %).
. Incubate sample(s) in the appropriate secondary Ab for 1 hour in a dark container.
. Rinse the sample(s) in PBS 4x 10 minutes, each.
. Place one drop of Vectashield onto the slide and cover with a coverslip
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer Tris-glycine, 150 mM NaCl, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Aliquot and store at -20 °C or -80 °C.
Supplier Images
Western Blotting (WB) image for anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (N-Term) antibody (ABIN269559) Detection of LC3B in treated U87-MG (human glioblastoma astrocytoma) lysates using AB...
Immunohistochemistry (IHC) image for anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (N-Term) antibody (ABIN269559) Staining of treated U373-MG (human glioblastoma) cells using ABIN153145.
Immunofluorescence (IF) image for anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (N-Term) antibody (ABIN269559) Immunofluorescent staining of treated U373-MG cells using ABIN153145. The nuclei were...
Product cited in: Myeku, Clelland, Emrani, Kukushkin, Yu, Goldberg, Duff: "Tau-driven 26S proteasome impairment and cognitive dysfunction can be prevented early in disease by activating cAMP-PKA signaling." in: Nature medicine, Vol. 22, Issue 1, pp. 46-53, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Santanam, Banach-Petrosky, Abate-Shen, Shen, White, DiPaola: "Atg7 cooperates with Pten loss to drive prostate cancer tumor growth." in: Genes & development, Vol. 30, Issue 4, pp. 399-407, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting,Immunohistochemistry

Bauckman, Mysorekar: "Ferritinophagy drives uropathogenic Escherichia coli persistence in bladder epithelial cells." in: Autophagy, Vol. 12, Issue 5, pp. 850-63, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting

Alán, Špaček, Pajuelo Reguera, Jabůrek, Ježek: "Mitochondrial nucleoid clusters protect newly synthesized mtDNA during Doxorubicin- and Ethidium Bromide-induced mitochondrial stress." in: Toxicology and applied pharmacology, Vol. 302, pp. 31-40, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting

Lee, Du, Stitham, Atteya, Lee, Xiang, Wang, Jin, Leslie, Spollett, Srivastava, Mannam, Ostriker, Martin, Tang, Hwa: "Inducing mitophagy in diabetic platelets protects against severe oxidative stress." in: EMBO molecular medicine, Vol. 8, Issue 7, pp. 779-95, 2016 (PubMed). (Sample species: Human). Further details: Flow Cytometry

Cemma, Grinstein, Brumell: "Autophagy proteins are not universally required for phagosome maturation." in: Autophagy, Vol. 12, Issue 9, pp. 1440-6, 2016 (PubMed). Further details: Western Blotting

Shui, Liu, Zhu, Wang: "Exogenous hydrogen sulfide attenuates cerebral ischemia-reperfusion injury by inhibiting autophagy in mice." in: Canadian journal of physiology and pharmacology, pp. 1-6, 2016 (PubMed).

Sousa, Biancur, Wang, Halbrook, Sherman, Zhang, Kremer, Hwang, Witkiewicz, Ying, Asara, Evans, Cantley, Lyssiotis, Kimmelman: "Pancreatic stellate cells support tumour metabolism through autophagic alanine secretion." in: Nature, Vol. 536, Issue 7617, pp. 479-83, 2016 (PubMed). Further details: Western Blotting

Qi, Man, Malireddi, Karki, Lupfer, Gurung, Neale, Guy, Lamkanfi, Kanneganti: "Cathepsin B modulates lysosomal biogenesis and host defense against Francisella novicida infection." in: The Journal of experimental medicine, Vol. 213, Issue 10, pp. 2081-97, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Western Blotting,Immunofluorescence

Zhou, Wen, Cheng, Ma, Li, Ren, Sheng, Dong, Lu, Hu, Wang: "Tumor-released autophagosomes induce IL-10-producing B cells with suppressive activity on T lymphocytes via TLR2-MyD88-NF-κB signal pathway." in: Oncoimmunology, Vol. 5, Issue 7, pp. e1180485, 2016 (PubMed). Further details: Flow Cytometry

Wang, Han, Sun, Feng et al.: "Cerebral ischemic post‑conditioning induces autophagy inhibition and a HMGB1 secretion attenuation feedback loop to protect against ischemia reperfusion injury in an oxygen glucose deprivation ..." in: Molecular medicine reports, Vol. 14, Issue 5, pp. 4162-4172, 2016 (PubMed). (Sample species: Rat (Rattus)). Further details: Immunocytochemistry,Immunofluorescence

Gschweitl, Ulbricht, Barnes, Enchev, Stoffel-Studer, Meyer-Schaller, Huotari, Yamauchi, Greber, Helenius, Peter: "A SPOPL/Cullin-3 ubiquitin ligase complex regulates endocytic trafficking by targeting EPS15 at endosomes." in: eLife, Vol. 5, pp. e13841, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting

Yang, Stevenson, Scott, Ismail: "Type I interferon contributes to noncanonical inflammasome activation, mediates immunopathology, and impairs protective immunity during fatal infection with lipopolysaccharide-negative ehrlichiae." in: The American journal of pathology, Vol. 185, Issue 2, pp. 446-61, 2015 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Bernard, Dieudé, Yang, Hamelin, Underwood, Hébert: "Autophagy fosters myofibroblast differentiation through MTORC2 activation and downstream upregulation of CTGF." in: Autophagy, Vol. 10, Issue 12, pp. 2193-207, 2015 (PubMed). Further details: Western Blotting

Xie, Koh, Price, White, Mehnert: "Atg7 Overcomes Senescence and Promotes Growth of BrafV600E-Driven Melanoma." in: Cancer discovery, Vol. 5, Issue 4, pp. 410-23, 2015 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Polletta, Vernucci, Carnevale, Arcangeli, Rotili, Palmerio, Steegborn, Nowak, Schutkowski, Pellegrini, Sansone, Villanova, Runci, Pucci, Morgante, Fini, Mai, Russo, Tafani: "SIRT5 regulation of ammonia-induced autophagy and mitophagy." in: Autophagy, Vol. 11, Issue 2, pp. 253-70, 2015 (PubMed).

Wengrod, Wang, Weiss, Zhong, Osman, Gardner: "Phosphorylation of eIF2α triggered by mTORC1 inhibition and PP6C activation is required for autophagy and is aberrant in PP6C-mutated melanoma." in: Science signaling, Vol. 8, Issue 367, pp. ra27, 2015 (PubMed). (Sample species: Human). Further details: Immunohistochemistry

Strohecker, Joshi, Possemato, Abraham, Sabatini, White: "Identification of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase as a novel autophagy regulator by high content shRNA screening." in: Oncogene, Vol. 34, Issue 45, pp. 5662-76, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting

Fjeld, Weiss, Lasher, Rosendahl, Chen, Johansson, Kirsten, Ruffert, Masson, Steine, Bugert, Cnop, Grützmann, Mayerle, Mössner, Ringdal, Schulz, Sendler, Simon, Sztromwasser, Torsvik, Scholz, Tjora et al.: "A recombined allele of the lipase gene CEL and its pseudogene CELP confers susceptibility to chronic pancreatitis. ..." in: Nature genetics, Vol. 47, Issue 5, pp. 518-22, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting

Sharifi, Mowers, Drake, Macleod: "Measuring autophagy in stressed cells." in: Methods in molecular biology (Clifton, N.J.), Vol. 1292, pp. 129-50, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting