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Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (N-Term) antibody

Details for Product No. ABIN269559, Supplier: Log in to see
Antigen
  • Map1lc3
  • zgc:56434
  • wu:fb60g11
  • map1lc3b
  • MGC76283
  • MAP1LC3B
  • ATG8F
  • LC3B
  • MAP1A/1BLC3
  • MAP1LC3B-a
  • 1010001C15Rik
  • Atg8
  • LC3b
  • Mpl3
  • zbs559
  • microtubule-associated protein 1 light chain 3 beta
  • map1lc3b
  • MAP1LC3B
  • LOC100361973
  • Map1lc3b
Epitope
N-Term
76
31
22
21
18
12
11
11
9
8
7
6
6
6
6
4
4
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Primate, Rat (Rattus), Zebrafish (Danio rerio)
243
99
68
32
32
27
26
25
12
12
5
4
1
Host
Rabbit
228
20
1
Clonality
Polyclonal
Conjugate
Un-conjugated
15
14
13
12
12
12
4
4
4
4
4
3
3
3
1
1
1
1
1
1
1
1
1
1
1
Application
Electron Microscopy (EM), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Simple Western (SimWes), Western Blotting (WB)
126
125
80
76
65
59
55
31
26
10
9
6
3
2
1
1
1
Options
Supplier
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Immunogen A synthetic peptide made to the N-terminal region of the human LC3B protein. [UniProt# Q9GZQ8]
Cross-Reactivity (Details) Zebrafish reactivity reported in scientific literature (PMID: 23724125). Canine,primate reactivity reported in scientific literature (PMID: 24027311) Other species have not been tested.
Purification Immunogen affinity purified
Alternative Name LC3B/MAP1LC3B (MAP1LC3B Antibody Abstract)
Background Gene Symbol: MAP1LC3B
Gene ID 81631
UniProt Q9GZQ8
Application Notes Western Blot 1:1000, Simple Western 1:100, Flow Cytometry 1:200, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 0.1-2 μg/mL, Immunohistochemistry-Paraffin 1:200-1:400, Immunohistochemistry-Frozen 1:400, Electron MicroscopyThis LC3 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry-frozen sections, Flow Cytometry and Western Blot where bands are seen ~17 and 19 kDa corresponding to LC3-II and LC3-I. Electron Microscopy was reported in scientific literature. In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for LC3B Antibody Fluorescent Staining
1. After washing sample(s) in PBS, fix for 20 minutes at room temperature.
. Wash sample(s) in PBS 3x 3 minutes, each.
. Block sample(s) with 5 % NGS (keep Triton X-100 at 0.1 %) for 60 minutes at room temperature
. Prepare primary anti-LC3B solution during this time at a 1:5,000 dilution with 1 %NGS (keep Triton X-100 at 0.1 %).
. Incubate sample(s) in the primary Ab overnight at 4C in a humidified containerNote: If thick tissue samples, Ab must be prepared in 0.5 % Triton X-100/PBSDay
. Wash sample(s) in PBS 3x 5 minutes with wells on. Prepare Alexa-fluor antibodies (Goat anti-Rabbit IgG H+L) as secondary Ab with 1 % NGS (keep Triton X-100 at 0.1 %).
. Incubate sample(s) in the appropriate secondary Ab for 1 hour in a dark container.
. Rinse the sample(s) in PBS 4x 10 minutes, each.
. Place one drop of Vectashield onto the slide and cover with a coverslip
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -80 °C,-20 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (N-Term) antibody (ABIN269559) Detection of LC3B in treated U87-MG (human glioblastoma astrocytoma) lysates using AB...
Immunohistochemistry (IHC) image for anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (N-Term) antibody (ABIN269559) Staining of treated U373-MG (human glioblastoma) cells using ABIN153145.
Immunofluorescence (IF) image for anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (N-Term) antibody (ABIN269559) Immunofluorescent staining of treated U373-MG cells using ABIN153145. The nuclei were...
Simple Western (SimWes) image for anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (N-Term) antibody (ABIN269559) Simple Western: LC3B/MAP1LC3B Antibody [ABIN153145] - Simple Western lane view shows ...
Immunohistochemistry (IHC) image for anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (N-Term) antibody (ABIN269559) Immunohistochemistry: LC3B/MAP1LC3B Antibody [ABIN153145] - LC3B staining in gliobast...
Flow Cytometry (FACS) image for anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (N-Term) antibody (ABIN269559) Flow Cytometry: LC3B/MAP1LC3B Antibody [ABIN153145] - Staining of NTERA-2 cells using...
Immunohistochemistry (IHC) image for anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (N-Term) antibody (ABIN269559) Immunohistochemistry: LC3B/MAP1LC3B Antibody [ABIN153145] - LC3B staining in treated ...
Western Blotting (WB) image for anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (N-Term) antibody (ABIN269559) Western Blot: LC3B/MAP1LC3B Antibody [ABIN153145] - analysis of LC3B in IB3-1 whole c...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (N-Term) antibody (ABIN269559) Immunohistochemistry-Paraffin: LC3B/MAP1LC3B Antibody [ABIN153145] - analysis of U87M...
Immunofluorescence (IF) image for anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (N-Term) antibody (ABIN269559) Immunocytochemistry/Immunofluorescence: LC3B/MAP1LC3B Antibody [ABIN153145] - LC3 ant...
Immunofluorescence (IF) image for anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (N-Term) antibody (ABIN269559) Immunocytochemistry/Immunofluorescence: LC3B/MAP1LC3B Antibody [ABIN153145] - IF Conf...
Immunofluorescence (IF) image for anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (N-Term) antibody (ABIN269559) Immunocytochemistry/Immunofluorescence: LC3B/MAP1LC3B Antibody [ABIN153145] - analysi...
Product cited in: Myeku, Clelland, Emrani, Kukushkin, Yu, Goldberg, Duff: "Tau-driven 26S proteasome impairment and cognitive dysfunction can be prevented early in disease by activating cAMP-PKA signaling." in: Nature medicine, Vol. 22, Issue 1, pp. 46-53, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Santanam, Banach-Petrosky, Abate-Shen, Shen, White, DiPaola: "Atg7 cooperates with Pten loss to drive prostate cancer tumor growth." in: Genes & development, Vol. 30, Issue 4, pp. 399-407, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting,Immunohistochemistry

Bauckman, Mysorekar: "Ferritinophagy drives uropathogenic Escherichia coli persistence in bladder epithelial cells." in: Autophagy, Vol. 12, Issue 5, pp. 850-63, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting

Alán, Špaček, Pajuelo Reguera, Jabůrek, Ježek: "Mitochondrial nucleoid clusters protect newly synthesized mtDNA during Doxorubicin- and Ethidium Bromide-induced mitochondrial stress." in: Toxicology and applied pharmacology, Vol. 302, pp. 31-40, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting

Lee, Du, Stitham, Atteya, Lee, Xiang, Wang, Jin, Leslie, Spollett, Srivastava, Mannam, Ostriker, Martin, Tang, Hwa: "Inducing mitophagy in diabetic platelets protects against severe oxidative stress." in: EMBO molecular medicine, Vol. 8, Issue 7, pp. 779-95, 2016 (PubMed). (Sample species: Human). Further details: Flow Cytometry

Cemma, Grinstein, Brumell: "Autophagy proteins are not universally required for phagosome maturation." in: Autophagy, Vol. 12, Issue 9, pp. 1440-6, 2016 (PubMed). Further details: Western Blotting

Shui, Liu, Zhu, Wang: "Exogenous hydrogen sulfide attenuates cerebral ischemia-reperfusion injury by inhibiting autophagy in mice." in: Canadian journal of physiology and pharmacology, pp. 1-6, 2016 (PubMed).

Sousa, Biancur, Wang, Halbrook, Sherman, Zhang, Kremer, Hwang, Witkiewicz, Ying, Asara, Evans, Cantley, Lyssiotis, Kimmelman: "Pancreatic stellate cells support tumour metabolism through autophagic alanine secretion." in: Nature, Vol. 536, Issue 7617, pp. 479-83, 2016 (PubMed). Further details: Western Blotting

Qi, Man, Malireddi, Karki, Lupfer, Gurung, Neale, Guy, Lamkanfi, Kanneganti: "Cathepsin B modulates lysosomal biogenesis and host defense against Francisella novicida infection." in: The Journal of experimental medicine, Vol. 213, Issue 10, pp. 2081-97, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Western Blotting,Immunofluorescence

Zhou, Wen, Cheng, Ma, Li, Ren, Sheng, Dong, Lu, Hu, Wang: "Tumor-released autophagosomes induce IL-10-producing B cells with suppressive activity on T lymphocytes via TLR2-MyD88-NF-κB signal pathway." in: Oncoimmunology, Vol. 5, Issue 7, pp. e1180485, 2016 (PubMed). Further details: Flow Cytometry

Wang, Han, Sun, Feng et al.: "Cerebral ischemic post‑conditioning induces autophagy inhibition and a HMGB1 secretion attenuation feedback loop to protect against ischemia reperfusion injury in an oxygen glucose deprivation ..." in: Molecular medicine reports, Vol. 14, Issue 5, pp. 4162-4172, 2016 (PubMed). (Sample species: Rat (Rattus)). Further details: Immunocytochemistry,Immunofluorescence

Gschweitl, Ulbricht, Barnes, Enchev, Stoffel-Studer, Meyer-Schaller, Huotari, Yamauchi, Greber, Helenius, Peter: "A SPOPL/Cullin-3 ubiquitin ligase complex regulates endocytic trafficking by targeting EPS15 at endosomes." in: eLife, Vol. 5, pp. e13841, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting

Yu, Xie, Cao, Yang, Yang, Lotze, Zeh, Kang, Tang: "The ferroptosis inducer erastin enhances sensitivity of acute myeloid leukemia cells to chemotherapeutic agents." in: Molecular & cellular oncology, Vol. 2, Issue 4, pp. e1054549, 2016 (PubMed). Method employed by authors: Western Blotting (WB)

Dickinson, Alevy, Malvin, Patel, Gunsten, Holtzman, Stappenbeck, Brody: "IL13 activates autophagy to regulate secretion in airway epithelial cells." in: Autophagy, Vol. 12, Issue 2, pp. 397-409, 2016 (PubMed). Method employed by authors: Western Blotting (WB)

Boman, Svensson, Boxer, Rojas, Seeley, Karydas, Miller, Kågedal, Svenningsson: "Distinct Lysosomal Network Protein Profiles in Parkinsonian Syndrome Cerebrospinal Fluid." in: Journal of Parkinson's disease, Vol. 6, Issue 2, pp. 307-15, 2016 (PubMed). Method employed by authors: Western Blotting (WB)

Vodicka, Chase, Iuliano, Tousley, Valentine, Sapp, Kegel-Gleason, Sena-Esteves, Aronin, DiFiglia: "Autophagy Activation by Transcription Factor EB (TFEB) in Striatum of HDQ175/Q7 Mice." in: Journal of Huntington's disease, Vol. 5, Issue 3, pp. 249-260, 2016 (PubMed).

Yang, Stevenson, Scott, Ismail: "Type I interferon contributes to noncanonical inflammasome activation, mediates immunopathology, and impairs protective immunity during fatal infection with lipopolysaccharide-negative ehrlichiae." in: The American journal of pathology, Vol. 185, Issue 2, pp. 446-61, 2015 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Bernard, Dieudé, Yang, Hamelin, Underwood, Hébert: "Autophagy fosters myofibroblast differentiation through MTORC2 activation and downstream upregulation of CTGF." in: Autophagy, Vol. 10, Issue 12, pp. 2193-207, 2015 (PubMed). Further details: Western Blotting

Xie, Koh, Price, White, Mehnert: "Atg7 Overcomes Senescence and Promotes Growth of BrafV600E-Driven Melanoma." in: Cancer discovery, Vol. 5, Issue 4, pp. 410-23, 2015 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Polletta, Vernucci, Carnevale, Arcangeli, Rotili, Palmerio, Steegborn, Nowak, Schutkowski, Pellegrini, Sansone, Villanova, Runci, Pucci, Morgante, Fini, Mai, Russo, Tafani: "SIRT5 regulation of ammonia-induced autophagy and mitophagy." in: Autophagy, Vol. 11, Issue 2, pp. 253-70, 2015 (PubMed).