Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (N-Term) antibody

Details for Product No. ABIN269559
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Antigen
Synonyms Map1lc3, zgc:56434, wu:fb60g11, map1lc3b, MGC76283, MAP1LC3B, ATG8F, LC3B, MAP1A/1BLC3, MAP1LC3B-a, 1010001C15Rik, Atg8, LC3b, Mpl3, zbs559
Epitope
N-Term
(26), (11), (9), (9), (6), (6), (6), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(117), (44), (35), (24), (24), (14), (13), (4), (2), (1)
Host
Rabbit
(107), (14), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(9), (7), (7), (7), (6), (5), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application
Western Blotting (WB), Immunoelectron Microscopy (IEM), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(64), (55), (32), (26), (23), (20), (20), (18), (12), (5), (2), (1), (1)
Pubmed 4 references available
Quantity 0.025 mL
Shipping to United States (Change)
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Catalog No. ABIN269559
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Immunogen A synthetic peptide made to the N-terminal region of the human LC3B protein.
Cross-Reactivity (Details) Zebrafish reactivity reported in scientific literature (PMID: 23724125). Canine,primate reactivity reported in scientific literature (PMID: 24027311) Other species have not been tested.
Purification affinity purified
Alternative Name LC3B
Background Autophagy is a process of intracellular bulk degradation in which cytoplasmiccomponents, including organelles, are sequestered within double-membrane vesiclesthat deliver the contents to the lysosome/vacuole for degradation. Duringmacroautophagy, the sequestering vesicles, termed autophagosomes, fuse with thelysosome or vacuole resulting in the delivery of an inner vesicle (autophagic body) intothe lumen of the degradative compartment. There are 16 proteins participating in theautophagy pathway in humans. The autophagy protein LC3, a mammalian homologueof Atg8, was originally identified as microtubule-associated protein 1 light chain 3. It is acomponent of both the MAP1A and MAP1B microtubule-binding domains and theheavy-chain independent regulation of LC3 expression may modify MAP1microtubule-binding activity during development. LC3 is the only known mammalianprotein identified that stably associates with the autophagosome membranes. LC3-I is cytosolic and LC3-II ismembrane bound and enriched in the autophagic vacuole fraction. The detection ofLC3-I to LC3-II conversion is a useful and sensitive marker for distinguishing autophagyin mammalian cells. Alternate Names: anti-Microtubule-associated protein 1 light chain 3 beta antibody, anti-MAP1A/MAP1BLC3 B antibody, anti-MAP1A/1B light chain 3 B antibody, anti-MAP1 light chain 3-likeprotein 2 antibody, anti-Autophagy-related protein LC3 B antibody,anti-Autophagy-related ubiquitin-like modifier LC3 B antibody.
Gene Symbol: MAP1LC3B
Gene ID 81631
UniProt Q9GZQ8
Research Area Cancer, Autophagy, Cytoskeleton
Application Notes This LC3 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry-frozen sections, Flow Cytometry and Western Blot where bands are seen ~17 and 19 kDa corresponding to LC3-II and LC3-I. Electron Microscopy was reported in scientific literature.
Recommended dilutions: Electron Microscopy, Flow Cytometry 1:200, Immunocytochemistry/Immunofluorescence 0.1-2 µg/mL, Immunohistochemistry, Immunohistochemistry-Frozen 1:400, Immunohistochemistry-Paraffin 1:200-1:400, Western Blot 1:1000
Protocol Protocol specific for LC3B Antibody Fluorescent Staining
1. After washing sample(s) in PBS, fix for 20 minutes at room temperature.
. Wash sample(s) in PBS 3x 3 minutes, each.
. Block sample(s) with 5 % NGS (keep Triton X-100 at 0.1 %) for 60 minutes at room temperature
. Prepare primary anti-LC3B solution during this time at a 1:5,000 dilution with 1 %NGS (keep Triton X-100 at 0.1 %).
. Incubate sample(s) in the primary Ab overnight at 4C in a humidified containerNote: If thick tissue samples, Ab must be prepared in 0.5 % Triton X-100/PBSDay
. Wash sample(s) in PBS 3x 5 minutes with wells on. Prepare Alexa-fluor antibodies (Goat anti-Rabbit IgG H+L) as secondary Ab with 1 % NGS (keep Triton X-100 at 0.1 %).
. Incubate sample(s) in the appropriate secondary Ab for 1 hour in a dark container.
. Rinse the sample(s) in PBS 4x 10 minutes, each.
. Place one drop of Vectashield onto the slide and cover with a coverslip
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer Tris-glycine, 150 mM NaCl, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Aliquot and store at -20 °C or -80 °C.
General Aoki, Takada, Kondo et al.: "Evidence that curcumin suppresses the growth of malignant gliomas in vitro and in vivo through induction of autophagy: role of Akt and extracellular signal-regulated kinase signaling pathways." in: Molecular pharmacology, Vol. 72, Issue 1, pp. 29-39, 2007 (PubMed).

Pyo, Nah, Kim et al.: "Compensatory activation of ERK1/2 in Atg5-deficient mouse embryo fibroblasts suppresses oxidative stress-induced cell death." in: Autophagy, Vol. 4, Issue 3, pp. 315-21, 2008 (PubMed).

Tafani, Schito, Anwar et al.: "Induction of autophagic cell death by a novel molecule is increased by hypoxia." in: Autophagy, Vol. 4, Issue 8, pp. 1042-53, 2008 (PubMed).

Gao, Yeh, Lu et al.: "OSU-03012, a novel celecoxib derivative, induces reactive oxygen species-related autophagy in hepatocellular carcinoma." in: Cancer research, Vol. 68, Issue 22, pp. 9348-57, 2008 (PubMed).

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