Western Blot: p27/Kip1 Antibody [ABIN269609] - Analysis of p27 protein in MCF7 whole cell lysate (lanes 1-3) (arrowhead). Separation was achieved using a 4-20% gradient gel. Blocking occurred using 5% BLOTTO. Primary antibody was diluted 1:500 in 1% BLOTTO. The membrane was washed and reacted with a 1:10,000 dilution of Dylight 800 conjugated Gt-a-Rabbit IgG.
Western Blot: p27/Kip1 Antibody [ABIN269609] - Lane 1: HEK293 pcDNA3. Lane 2: HEK293 pcDNA3 Rb2wt. Lane 3: HEK293 pcDNA3 Rb2S941A. Lane 4: HEK293 pcDNA3 Rb2 PM19. Load: 30 ug per lane. Primary antibody: p27 antibody diluted in 1%BSA at 1:2000 for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 27 kDa for p27.